We explore the assumption, widely used in many astrophysical calculations, that the stellar initial mass function (IMF) is universal across all galaxies. By considering both a canonical Salpeter-like IMF and a non-universal IMF, we are able to compare the effect of different IMFs on multiple observables and derived quantities in astrophysics. Specifically, we consider a non-universal IMF which varies as a function of the local star formation rate, and explore the effects on the star formation rate density (SFRD), the extragalactic background light, the supernova (both core-collapse and thermonuclear) rates, and the diffuse supernova neutrino background. Our most interesting result is that our adopted varying IMF leads to much greater uncertainty on the SFRD at 𝑧 ≈ 2 − 4 than is usually assumed. Indeed, we find a SFRD (inferred using observed galaxy luminosity distributions) that is a factor of 3 lower than canonical results obtained using a universal Salpeter-like IMF. Secondly, the non-universal IMF we explore implies a reduction in the supernova core-collapse rate of a factor of ∼ 2, compared against a universal IMF. The other potential tracers are only slightly affected by changes to the properties of the IMF. We find that currently available data do not provide a clear preference for universal or non-universal IMF. However, improvements to measurements of the star formation rate and core-collapse supernova rate at redshifts 𝑧 2 may offer the best prospects for discernment.