Prospects for increasing the utilization of cattle embryo transfer by small‐scale milk and meat producers in tropical regions

Contreras, David A.; Galina, C.S.; Chenoweth, Peter J.

doi:10.1111/rda.14015

Cited by 7 publications

(6 citation statements)

References 42 publications

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“…The application of the ex situ program for the conservation of oocytes, spermatozoa, and embryos has a significant advantage, allowing their development to be resumed at the desired time [ 23 , 24 ]. Contreras, Galina, and Chenoweth [ 25 ] reported that cryoconservation has a low-efficiency due to the high risk of damaging reproductive materials. For this reason, our initial research mainly focused on the production of embryos and birth rates.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Fertilization in Kazakh Whiteheaded Cattle: A Comparative Study

Seisenov,

Duimbayev,

Kazhgaliyev

et al. 2023

Life

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In vitro fertilization (IVF) technologies have great potential in the preservation of endangered species. In the current study, an IVF experiment was carried out to evaluate whether reproductive technologies are suitable for Kazakh Whiteheaded cattle, aimed at preserving this breed whose population has reduced drastically over the last thirty years. The reproduction characteristics of Kazakh Whiteheaded cows were compared to Aberdeen Angus cows. Transvaginal ultrasound-guided ovum pick up sessions were carried out followed by in vitro embryo production and embryo transfer and pregnancy diagnosis. The total and viable oocytes per OPU procedure were 12.8 ± 1.18 and 8.7 ± 0.85 for the Aberdeen Angus breed, and 8.8 ± 1.04 and 6.2 ± 0.83 for the Kazakh Whiteheaded breed. Similarly, the mean number of cleaved oocytes and morula/blastocyst stage embryos produced by OPU/IVF were 4.8 ± 0.49 and 1.4 ± 0.15 for the Aberdeen Angus breed, and 2.4 ± 0.46 and 0.18 ± 0.05 for the Kazakh Whiteheaded breed (p ≤ 0.02). From fifty Kazakh Whiteheaded donor animals, 2585 oocytes were aspirated following six ovum pick up sessions. One thousand eight hundred and seventy-six (72.5%) oocytes were chosen for maturation and were further fertilized. The number of embryos cleaved was 720 (38.3% out of oocytes fertilized) on day four post-fertilization. Of these cleaved embryos, 56 (7.5%) developed into the late morula/blastocyst stage on day seven post-fertilization, averaging 1.12 embryos per donor animal. Pregnancy was detected in 12 recipients; 4 healthy calves have been born to date. The outcomes of our study have demonstrated that reproductive technologies can be applicable in preserving the endangered Kazakh Whiteheaded cattle. The findings in this report will enhance knowledge of the reproductive characteristics of endangered domestic animals and help develop sophisticated reproductive protocols for animals with unique reproductive mechanisms.

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Section: Discussionmentioning

confidence: 99%

In Vitro Fertilization in Kazakh Whiteheaded Cattle: A Comparative Study

Seisenov,

Duimbayev,

Kazhgaliyev

et al. 2023

Life

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“…More than one and a half million bovine embryos were produced in vitro worldwide in 2021 2 , but 60% had to be transferred fresh due to the lack of a reliable cryopreservation method that results in favorable pregnancy rates 2,7,8 . Consequently, a large number of embryo recipient animals must be available, resulting in increased costs 9,10 . Predicting blastocyst yield and optimization of a vitrification protocol would allow to decrease the number of recipients per calf born and to optimize the logistics of the IVEP process.…”

Section: Embryo Morphokinetics Derived From Fresh and Vitrified Bovin...mentioning

confidence: 99%

Embryo morphokinetics derived from fresh and vitrified bovine oocytes predict blastocyst development and nuclear abnormalities

Angel‐Velez

Coster

Azari‐Dolatabad

et al. 2023

Sci Rep

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Embryo development is a dynamic process and critical stages may go unnoticed with the use of traditional morphologic assessments, especially the timing of embryonic divisions and aberrant zygotic cleavage patterns. Bovine embryo development is impaired after oocyte vitrification, but little is known about the underlying morphokinetic behavior. Here, bovine zygotes from fresh (n = 708) and vitrified oocytes (n = 182) were monitored by time-lapse imaging and the timing and nature of early blastomere divisions were modeled to find associations with blastocyst development at day 8. The predictive potential of morphokinetic parameters was analyzed by logistic regression and receiver operating characteristic curve analysis to determine optimal cut-off values. Lag-phase was highly correlated with embryo development. Remarkably, 100% of zygotes that reached the blastocyst stage showed a lag-phase. Fast first cleavage increased the chance of blastocyst development to 30% with a cut-off of 32 h and 22 min. Aberrant zygotic cleavage events, including multipolar division, unequal blastomere sizes, and membrane ruffling resulted in decreased blastocyst development. Multipolar division leads to uneven blastomeres, which was associated with anuclear and multinuclear blastomeres, indicating genome segregation errors. Moreover, we described for the first time morphokinetics of embryos derived from vitrified bovine oocytes. Vitrification severely affected blastocyst development, although lower cryoprotectant concentration in equilibration solutions seems to be less detrimental for embryo yield. Impaired development was linked to slow cleavages, lower lag-phase incidence, and increased early embryonic arrest. Typically, less than 15% of the embryos produced from vitrified oocytes reached more than eight cells. Interestingly, the rate of abnormal first cleavage events was not affected by oocyte vitrification. In conclusion, time to first cleavage, the presence of a lag-phase, and the absence of aberrant zygotic cleavage were the best predictors of bovine blastocyst development for both fresh and vitrified oocytes.

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“…In a recent review, Contreras et al [ 112 ] pointed out several shortcomings associated with applying embryo transfer in small community farmers. The advantage of placing F1 embryos in F1 recipients is that it would eliminate the need for crossbreeding cattle, which is difficult and time-consuming (see the section on Genetics).…”

Section: Breeding Proceduresmentioning

confidence: 99%

Dual-Purpose Cattle Raised in Tropical Conditions: What Are Their Shortcomings in Sound Productive and Reproductive Function?

Galina

Geffroy

2023

Animals

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Dual-purpose husbandry might well be the most commonly employed cattle management system in tropical regions worldwide. The advantages of producing both meat and milk, although in reduced quantities, gives an edge to the farmer in coping with the volatile economic conditions that prevail in the region. Herein, we discuss the different methods of cattle management under tropical conditions based on the financial and social structure of this system. An account of the sanitary and nutritional conditions available to the farmers and how these factors affect the profitability of the enterprise will also be given. Finally, we will discuss how these systems can take advantage of several biotechnological procedures, and how these tools (such as controlled natural mating, artificial insemination, and embryo transfer) affect reproductive outcomes. The present review will mainly concentrate on production systems located less than 1000 m above sea level, as the problems and shortcomings of cattle raised above this arbitrary landmark are quite different.

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Prospects for increasing the utilization of cattle embryo transfer by small‐scale milk and meat producers in tropical regions

Cited by 7 publications

References 42 publications

In Vitro Fertilization in Kazakh Whiteheaded Cattle: A Comparative Study

In Vitro Fertilization in Kazakh Whiteheaded Cattle: A Comparative Study

Embryo morphokinetics derived from fresh and vitrified bovine oocytes predict blastocyst development and nuclear abnormalities

Dual-Purpose Cattle Raised in Tropical Conditions: What Are Their Shortcomings in Sound Productive and Reproductive Function?

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