2019
DOI: 10.1002/ps.5588
|View full text |Cite
|
Sign up to set email alerts
|

Prospects, challenges and current status of RNAi through insect feeding

Abstract: RNA interference is a phenomenon in which the introduction of double‐stranded RNA (dsRNA) into cells triggers the degradation of the complementary messenger RNA in a sequence‐specific manner. Suppressing expression of vital genes could lead to insect death, therefore this technology has been considered as a potential strategy for insect pest control. There are three main routes of dsRNA administration into insects: (i) injections to the hemolymph, (ii) topical, and (iii) feeding. In this review, we focus on ds… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

1
134
0
3

Year Published

2019
2019
2022
2022

Publication Types

Select...
7

Relationship

0
7

Authors

Journals

citations
Cited by 129 publications
(149 citation statements)
references
References 132 publications
1
134
0
3
Order By: Relevance
“…Several nanoparticles such as liposomes, guanylated polymers, quantum dots, branched amphiphilic peptide capsules (BAPCs), core shell nanoparticles have been used in various insects to enhance the RNAi effect (Avila et al, 2018;Zhang et al, 2015;Christiaens et al, 2018). The association of between nanoparticles and target dsRNA is mediated by electrostatic interaction which involves binding of amino group of nanoparticle with the phosphate group of dsRNA (Kunte et al, 2020). The conjugation between dsRNA and CQD particles may be due to polyethyleneimine (PEI) which is a cationic polymer and possess binding affinity to the nucleic acid to form a particulate complex which results in a decrease in the size of target dsRNA resulting in faster transfection efficiency and improved stability (Tros de Ilarduya et al, 2010).…”
Section: Results and Discussion Dsrna Degradation Studies And Enhancimentioning
confidence: 99%
“…Several nanoparticles such as liposomes, guanylated polymers, quantum dots, branched amphiphilic peptide capsules (BAPCs), core shell nanoparticles have been used in various insects to enhance the RNAi effect (Avila et al, 2018;Zhang et al, 2015;Christiaens et al, 2018). The association of between nanoparticles and target dsRNA is mediated by electrostatic interaction which involves binding of amino group of nanoparticle with the phosphate group of dsRNA (Kunte et al, 2020). The conjugation between dsRNA and CQD particles may be due to polyethyleneimine (PEI) which is a cationic polymer and possess binding affinity to the nucleic acid to form a particulate complex which results in a decrease in the size of target dsRNA resulting in faster transfection efficiency and improved stability (Tros de Ilarduya et al, 2010).…”
Section: Results and Discussion Dsrna Degradation Studies And Enhancimentioning
confidence: 99%
“…Efficient delivery methods have been described in several reports on the down‐regulation of plant endogenes and transgenes by the external application of naked dsRNAs or dsRNAs protected by liposome, artificial extracellular vesicles (EV) or complexed with nanoparticles or a protein carrier . Nanocarriers are increasingly being used as effective translational tools in achieving commercial viability of sprayable RNA‐based biocontrol compounds . As such, the combination of RNAi technology and nanotechnology is perceived as a game changer in the crop protection industry .…”
Section: Rna‐based Biocontrol Compounds For External Applicationmentioning
confidence: 99%
“…[15][16][17][18][19] Nanocarriers are increasingly being used as effective translational tools in achieving commercial viability of sprayable RNA-based biocontrol compounds. 20 As such, the combination of RNAi technology and nanotechnology is perceived as a game changer in the crop protection industry. 21 An example is the use of layered double hydroxide clay nanoparticles (BioClay) to deliver dsRNA as a stable application with increased longevity, highlighting the significance of nanoparticles as delivery vehicles, and the progress towards translation from the lab to the field.…”
Section: Rna-based Biocontrol Compounds For External Applicationmentioning
confidence: 99%
“…After ingestion of dsRNA molecules, a successful RNAi response depends on a variety of factors that will be discussed below, including: stability of dsRNA in the gut (affected by gut pH and nucleases), dsRNA length and concentration, target gene, arthropod species and the life-stage exposed (Katoch et al, 2013;Scott et al, 2013;Davis-Vogel et al, 2018;Cooper et al, 2019;Kunte et al, 2020).…”
Section: Hazard Posed By Dsrnamentioning
confidence: 99%
“…The RNAi trait is combined with the Cry3Bb1 protein for improved target pest control and resistance management (Levine et al, 2015;Head et al, 2017). Second, the dsRNA molecules can be applied externally, for example in irrigation water or through trunk injections (Hunter et al, 2012;Li et al, 2015a;Niu et al, 2018;Kunte et al, 2020), in food-baits (Zhou et al, 2008;Zhang et al, 2010), by using delivery systems such as micro-organisms, viruses, nanocarriers (Kunte et al, 2020;Vogel et al, 2019), or topically as spray applications (San Miguel and Scott, 2016).…”
Section: Introductionmentioning
confidence: 99%