Prospective Study on the Treatment of Lower-Extremity Chronic Venous and Mixed Ulcers Using Tissue-Engineered Skin Substitute Made by the Self-assembly Approach

Boa, Olivier; Cloutier, Chanel Beaudoin; Genest, Hervé; Labbé, Raymond; Rodrigue, Bertrand; Soucy, Jacques; Roy, Michel; Arsenault, Frédéric; Ospina, Carlos E.; Dubé, Nathalie; Rochon, Marie-Hélène; Larouche, Danielle; Moulin, Véronique J.; Germain, Lucie; Auger, François A.

doi:10.1097/01.asw.0000433102.48268.2a

Cited by 42 publications

(34 citation statements)

References 26 publications

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“…7,12 Briefly, fibroblasts were first expanded for 7 days in DMEM 10% FCS. After expansion (7 days), fibroblasts were seeded in flasks (Falcon) at 4 X 10 3 cells/cm 2 and cultured for 25 days in DMEM 10% FCS 50 µg/ml ascorbic acid.…”

Section: Production Of Self-assembled Skin Substitutes (Sass)mentioning

confidence: 99%

“…Our group previously presented a bilayered self-assembled skin substitute (SASS) made of a dermal substitute underlying a fully differentiated epidermis, which generated an engineered tissue similar in structure and function to native human skin [4][5][6] for clinical use in wound healing 7 and burn treatment. 8,9 SASS is currently used in a clinical trial for the permanent coverage of full-thickness wounds (ClinicalTrials.gov NCT02350205).…”

Section: Introductionmentioning

confidence: 99%

“…12 Three dermal sheets were superimposed to form a tissue-engineered dermal template comprised of fibroblasts and their self-secreted extracellular matrix, and cultured for a week to allow an appropriate cohesion between the sheets. 7 Following this culture period, the dermal templates were decellularized using two cycles of osmotic shock, followed by a rinsing and a dehydration process. 21 The dermal templates were first immersed in hypo-osmolar sterile apyrogen water (Milli-Q, EMD Millipore, Bellerica, MA, USA) for five hours at 4°C.…”

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confidence: 99%

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Production of a Bilayered Self-Assembled Skin Substitute Using a Tissue-Engineered Acellular Dermal Matrix

Cloutier

Guignard

Bernard

et al. 2015

Tissue Engineering Part C: Methods

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Our bilayered self-assembled skin substitutes (SASS) are skin substitutes showing a structure and functionality very similar to native human skin. These constructs are used, in life threatening burn wounds, as permanent autologous grafts for the treatment of such affected patients even though their production is exacting. We thus intended to shorten their current production time to improve their clinical applicability. A self-assembled decellularized dermal matrix was used. It allowed the production of an autologous skin substitute from patient's cells. The characterization of SASS reconstructed using a decellularized dermal matrix (SASS-DM) was performed by histology, immunofluorescence, transmission electron microscopy and uniaxial tensile analysis.Using the SASS-DM, it was possible to reduce the standard production time from about eight to four weeks and a half. The structure, cell differentiation and mechanical properties of the new skin substitutes were shown to be similar to the SASS. The decellularization process had no influence on the final microstructure and mechanical properties of the dermal matrix. This model, by enabling the production of a skin substitute in a shorter time frame without compromising its intrinsic tissue properties, represents a promising addition to the currently available burn and wound treatments.

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Section: Production Of Self-assembled Skin Substitutes (Sass)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Production of a Bilayered Self-Assembled Skin Substitute Using a Tissue-Engineered Acellular Dermal Matrix

Cloutier

Guignard

Bernard

et al. 2015

Tissue Engineering Part C: Methods

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“…The skin substitute was produced by the self-assembly method, an approach of tissue engineering based on the formation of ECM-rich cell sheets through the stimulation of fibroblasts with ascorbic acid 34 . It allows the production of human skin substitutes (epidermis and dermis) whose clinical relevance has already been well demonstrated by our group 35,36 . We incorporated human microvascular endothelial cells to recapitulate the formation of blood and lymphatic capillaries in the dermal compartment, and then melanoma spheroids were added to the tissue.…”

Section: Introductionmentioning

confidence: 91%

Tissue-engineered 3D melanoma model with blood and lymphatic capillaries for drug development

Bourland

Fradette

Auger

2018

Sci Rep

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While being the rarest skin cancer, melanoma is also the deadliest. To further drug discovery and improve clinical translation, new human cell-based in vitro models are needed. Our work strives to mimic the melanoma microenvironment in vitro as an alternative to animal testing. We used the self-assembly method to produce a 3D human melanoma model exempt of exogenous biomaterial. This model is based on primary human skin cells and melanoma cell lines while including a key feature for tumor progression: blood and lymphatic capillaries. Major components of the tumor microenvironment such as capillaries, human extracellular matrix, a stratified epidermis (involucrin, filaggrin) and basement membrane (laminin 332) are recapitulated in vitro. We demonstrate the persistence of CD31+ blood and podoplanin+/LYVE-1+ lymphatic capillaries in the engineered tissue. Chronic treatment with vemurafenib was applied to the model and elicited a dose-dependent response on proliferation and apoptosis, making it a promising tool to test new compounds in a human-like environment.

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“…We have developed a new way to obtain a very dense tissue using only cells that is based on the capability of cells to produce and organize their own extracellular matrix. This method is called the self-assembly method and has been used to reconstruct skin [5,6], adipose tissue [7] and blood vessels [8]. However, production of these tissues is a lengthy process, which is a major drawback to using this method to reconstitute tissues or organs and quickly treat patients at low cost.…”

Section: Introductionmentioning

confidence: 99%

Role of seaweed laminaran from Saccharina longicruris on matrix deposition during dermal tissue-engineered production

Ayoub

Pereira

Rioux

et al. 2015

International Journal of Biological Macromolecules

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Prospective Study on the Treatment of Lower-Extremity Chronic Venous and Mixed Ulcers Using Tissue-Engineered Skin Substitute Made by the Self-assembly Approach

Abstract: The authors' study suggests that the SASS used as a biological dressing is a promising treatment for hard-to-heal chronic venous and mixed ulcers that are unresponsive to compression therapy.

Cited by 42 publications

References 26 publications

Production of a Bilayered Self-Assembled Skin Substitute Using a Tissue-Engineered Acellular Dermal Matrix

Production of a Bilayered Self-Assembled Skin Substitute Using a Tissue-Engineered Acellular Dermal Matrix

Tissue-engineered 3D melanoma model with blood and lymphatic capillaries for drug development

Role of seaweed laminaran from Saccharina longicruris on matrix deposition during dermal tissue-engineered production

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