Prospective study for the development of emulsion systems containing natural oil products

Xavier, Francisco Humberto; Silva, K. G. H.; Farias, I. E. G.; Morais, Andreza R V; Alencar, Éverton do Nascimento; Araújo, Ivonete Batista de; Oliveira, Anselmo Gomes de; Egito, Eryvaldo Sócrates Tabosa do

doi:10.1016/s1773-2247(12)50061-4

Cited by 9 publications

(7 citation statements)

References 27 publications

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“…Creaming, phase separation or other signals of unstable behavior were not observed even after 30 days, also observing that this formulation presented a bluish reflection, characteristic for nanoemulsions (Forgiarini et al, 2000). A prospective study with copaiba oleoresin performed by Xavier-Júnior et al (2012) using different blends of surfactants indicated a required HLB value of 14.8 for the oil phase, which is in accordance with our results. Required HLB value of the oil phase is one of most important parameters that should be considered during the development of nanoemulsions (Schmidts et al, 2010;Fernandes et al 2013).…”

Section: Resultssupporting

confidence: 91%

Development of a larvicidal nanoemulsion with Copaiba (Copaifera duckei) oleoresin

Rodrigues

Ferreira

Vilhena

et al. 2014

Revista Brasileira de Farmacognosia

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CopaibaLarvicidal Nanoemulsion Oleoresin A B S T R A C T Copaiba (Copaifera duckei Dwyer, Fabaceae) oleoresin is an important Amazonian raw material. Despite its insecticidal potential, poor water solubility remains a challenge for the development of effective and viable products. Nanotechnology has emerged as a promising area to solve this problem, especially oil-in-water nanoemulsions. On this context, the aim of the present study was to develop oil-in-water nanoemulsions using copaiba oleoresin dispersed through a high internal phase; and evaluate its potential insecticidal action against Aedes aegypti larvae. Overall, 31 formulations were prepared, ranging from 11.5 ± 0.2 to 257.3 ± 4.1 nm after one day of manipulation. Some of them reached small mean droplet sizes (< 200 nm) and allowed achievement of a nanoemulsion region. The formulation consisted of 5% (w/w) of copaiba oil, 5% (w/w) of surfactant and 90% (w/w) of water, which presented mean droplet size of 145.2 ±0.9 nm and polidispersity of 0.378 ± 0.009 after one day of manipulation, and these were evaluated for larvicidal potential. According to mortality level (250 ppm -93.3 after 48 h), this nanoemulsion was classified as a promising insecticidal agent against Aedes aegypti larvae. The present study allowed the development of low-cost ecofriendly green natural-based nanoformulations with potential larvicidal activity, using a nanobiotechnology approach.

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Section: Resultssupporting

confidence: 91%

Development of a larvicidal nanoemulsion with Copaiba (Copaifera duckei) oleoresin

Rodrigues

Ferreira

Vilhena

et al. 2014

Revista Brasileira de Farmacognosia

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“…These results suggest a microbial contamination or degradation of the fatty acids with a long carbon chain presented in the bullfrog oil, which can be oxidized to form hydroperoxides or hydrolysis of triglycerides [11,39,40,41]. Regarding the electrical conductivity, the high value found allowed us to speculate that the oil was dispersed in the water phase, producing an oil-in-water (O/W) nanoemulsion [42,43].…”

Section: Resultsmentioning

confidence: 99%

“…Neither sample showed changes in either the color or the odor. The centrifuge resistance immediately after system preparations did not demonstrate changes in either sample, predicting suitable physical stability of the different emulsions [42]. The freeze/thaw cycle study revealed that the basic nanoemulsion presented phase separation after the second cycle, while the topical nanoemulsion remained stable until the sixth cycle, indicating that the addition of excipients in the topical formulation enhanced its stability under large temperature variations [59].…”

Section: Resultsmentioning

confidence: 99%

“…Thereby, 90 different formulations were produced using Ultra Turrax ® -T-18 stirring at 11,000 r.p.m for 10 min each. The systems were characterized by visual inspection in order to identify nanoemulsions, which is optically translucent and scatter light due to the Tyndall effect [42,53], microemulsion in which the system presented transparent appearance [45,52] and emulsion systems that showed a milky aspect [9,17]. Gels were defined as systems that showed clear visual appearance and high viscosity.…”

Section: Methodsmentioning

confidence: 99%

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New Trends on Antineoplastic Therapy Research: Bullfrog (Rana catesbeiana Shaw) Oil Nanostructured Systems

et al. 2016

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Bullfrog oil is a natural product extracted from the Rana catesbeiana Shaw adipose tissue and used in folk medicine for the treatment of several diseases. The aim of this study was to evaluate the extraction process of bullfrog oil, to develop a suitable topical nanoemulsion and to evaluate its efficacy against melanoma cells. The oil samples were obtained by hot and organic solvent extraction processes and were characterized by titration techniques and gas chromatography mass spectrometry (GC-MS). The required hydrophile-lipophile balance and the pseudo-ternary phase diagram (PTPD) were assessed to determine the emulsification ability of the bullfrog oil. The anti-tumoral activity of the samples was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay for normal fibroblast (3T3) and melanoma (B16F10) cell lines. Both extraction methods produced yielded around 60% and the oil was mainly composed of unsaturated compounds (around 60%). The bullfrog oil nanoemulsion obtained from PTPD presented a droplet size of about 390 nm and polydispersity = 0.05 and a zeta potential of about´25 mV. Both the bullfrog oil itself and its topical nanoemulsion did not show cytotoxicity in 3T3 linage. However, these systems showed growth inhibition in B16F10 cells. Finally, the bullfrog oil presented itself as a candidate for the development of pharmaceutical products free from cytotoxicity and effective for antineoplastic therapy.

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“…-Nanoemulsions were subjected to alternate six freeze and thaw cycles for a period of 12 days. 10 g of nanoemulsions were stored in sealed tubes for 24 h in a freezer at -5 °C, followed by another period of 24 h at 45 °C according to Xavier-Júnior et al (16). After each cycle, the macroscopic aspect was analyzed as described above (2.5.1.…”

Section: Stability Studiesmentioning

confidence: 99%

Therapeutic bullfrog oil-based nanoemulsion for oral application: Development, characterization and stability

et al. 2018

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The aim of this study was to develop, optimize, and characterize a stable therapeutic bullfrog oil based nanoemulsion for oral application using a rational experimental design approach. The optimized oral nanoemulsion contained 0.2 % sodium benzoate and 0.02 % propyl-paraben as preservatives; 0.1 % sucralose and 0.4 % acesulfam K as sweeteners and 0.1 % tutti-frutti as flavoring to mask the unpleasant organoleptic characteristics of bullfrog oil. The oral O/W-nanoemulsion showed the droplet size, PDI, zeta potential, and pH of 410 ± 8 nm, 0.20 ± 0.02, –38 ± 2.5 mV, and 6.43 ± 0.05, respectively. The optimized oral nanoemulsion showed a milky single-phase and optimal physical stability at 25 °C for 90 days. Indeed, higher oxidation induction time and lower formation of peroxides in the oral nanoemulsion were responsible for improving its stability. A therapeutic delivery system containing bullfrog oil for oral application was successfully developed and optimized with ideal thermo-oxidative stability.

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Prospective study for the development of emulsion systems containing natural oil products

Cited by 9 publications

References 27 publications

Development of a larvicidal nanoemulsion with Copaiba (Copaifera duckei) oleoresin

Development of a larvicidal nanoemulsion with Copaiba (Copaifera duckei) oleoresin

New Trends on Antineoplastic Therapy Research: Bullfrog (Rana catesbeiana Shaw) Oil Nanostructured Systems

Therapeutic bullfrog oil-based nanoemulsion for oral application: Development, characterization and stability

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