Prospective studies on nasopharyngeal carcinoma in epstein‐barr virus IgA/VCA antibody‐positne persons in Wuzhou city, china

Zeng, Yi; Zhang, L. G.; Wu, Yixuan; Huang, Yimin; Huang, Nuan-Ya; Li, J. Y.; Wang, Y. B.; Jiang, Matthew; Fang, Zhao-Yin; Meng, Ningqin

doi:10.1002/ijc.2910360505

Cited by 171 publications

(129 citation statements)

References 3 publications

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“…Another 6.5% has a moderate level of risk with odds ratios of 4 to 10. This is similar to the risk level sustained by those who have elevated levels of VCA IgA antibody determined by the traditional test, 5,7,[15][16][17] and the knowledge of the risk serves to alert these individuals and their physicians to early symptoms of NPC. In addition, the combined test results serve to identify a third group comprising an estimated 0.4% of HS, who are at high risk of NPC with an odds ratio of 138.…”

Section: Discussionmentioning

confidence: 67%

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Assessing the risk of nasopharyngeal carcinoma on the basis of EBV antibody spectrum

Cheng

Chan

Chen

et al. 2001

Intl Journal of Cancer

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We have evaluated the performance of 3 new EBV ELISA for the diagnosis of nasopharyngeal carcinoma (NPC). The tests were specific for EBNA 1 IgA, EBNA 1 IgG and zta IgG, respectively. Their distinct antigenic specificity permits these assays to be used in concert in an approach that differentiates patients and apparently healthy subjects on the basis of their antibody spectrum. By so exploiting a distinguishing feature of NPC first described by the late Werner Henle that the patients sustain high levels of a broad spectrum of serum EBV antibodies, this approach achieved a sensitivity of 92% and a specificity of 93%, surpassing the performance of each of these assays individually. The enhanced performance is especially useful in population screening. It was shown that relative risk of NPC sustained by apparently healthy subjects residing in a high incidence area for NPC in the Pearl River estuary in Southern China may vary according to EBV antibody spectrum. The risk of the cancer was markedly reduced with odds ratios of 0.009 for 59% of those who had low level of all 3 antibodies. The risk was increased as antibody spectrum broadens and the risk was the highest with an odds ratio of 138 for 0.4% of those who had high levels of all 3 antibodies. Thus, EBV antibody spectrum may serve to guide follow-up measures for early detection of the cancer and/or risk counseling according to level of the risk of the cancer sustained by the screened individuals.

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Section: Discussionmentioning

confidence: 67%

“…Previous population studies carried out in high-incidence areas for NPC estimated the occurrence of the cancer among apparently healthy subjects having an elevated serum level of VCA IgA to be about 1% per year. 4,5,7 In practice, this serves to alert the individuals and clinicians to early symptoms of the cancer.…”

Section: Discussionmentioning

confidence: 99%

Assessing the risk of nasopharyngeal carcinoma on the basis of EBV antibody spectrum

Cheng

Chan

Chen

et al. 2001

Intl Journal of Cancer

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“…1 Although several previous studies [2][3][4][5] have reported on the use of EBV serology for screening and early detection of NPC, the role of EBV DNA detection as a screening test for NPC remains to be defined. Circulating cell-free EBV DNA is detectable in the plasma of 96% of NPC patients 6 by quantitative real-time polymerase chain reaction (PCR) and has been shown to be a useful marker in prognostication 7 as well as residual disease detection.…”

Section: Dear Sirmentioning

confidence: 99%

Plasma Epstein‐Barr virus (EBV) DNA: Role as a screening test for nasopharyngeal carcinoma (NPC)?

Wong

Lai

Tsui

et al. 2005

Intl Journal of Cancer

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Dear Sir,We read with interest the recent article in this journal on screening for family members of nasopharyngeal carcinoma (NPC) patients by serologic tests against Epstein-Barr virus (EBV). 1 Although several previous studies [2][3][4][5] have reported on the use of EBV serology for screening and early detection of NPC, the role of EBV DNA detection as a screening test for NPC remains to be defined. Circulating cell-free EBV DNA is detectable in the plasma of 96% of NPC patients 6 by quantitative real-time polymerase chain reaction (PCR) and has been shown to be a useful marker in prognostication 7 as well as residual disease detection. 8 Studies on EBV DNA detection, however, are performed on known NPC patients but not in the general population. We report our preliminary results on plasma EBV DNA detection, in association with EBV serology, as screening tests for NPC in the setting of routine health assessment.In the 6-month period from July to December 2004, a total of 1,475 blood samples were referred from the Health Assessment Centre of our hospital for detection of EBV status as part of body checkup. They comprised 875 male patients and 600 female patients with a median age of 49 years (range 16-92 years). None of them had prior history of EBV-related malignancies. All patients were tested for cell-free plasma EBV DNA level, and when the result was positive, serum samples were retrieved for EBV serologic testing.The RealArt EBV LC PCR Kit (Artus, Hamburg, Germany), working on the LightCycler instrument (Roche Diagnostics, Mannheim, Germany), was employed for plasma EBV DNA detection. The EBV LC PCR Master reagent contained reagents and enzymes for the specific amplification of a 97 bp region of the EBNA1 gene of the EBV genome. Quantification standards were provided with the kit and were included in each run so as to generate the standard curve for EBV load determination. An external quantified EBV viral DNA control (Advanced Biotechnologies, Columbia, MD) was included in each run to check the performance of the assay. Serum EBV VCA IgA and EA IgA antibody levels were determined by indirect immunofluorescence assay on Raji cells and B95-8 cells induced to express EA and VCA, respectively (purchased from the Department of Microbiology, The University of Hong Kong). Antibody titer was defined as the highest dilution that yielded a positive fluorescent signal. Cutoff values for positivity were set at 1:40 for VCA IgA and 1:10 for EA IgA.The presence of EBV DNA was detected in 33 patients (2.24%). Among them, 4 were positive for VCA IgA in which 1 patient was also positive for EA IgA. The clinical records of these patients were reviewed. The only patient with detectable EBV DNA (118 copies/mL) and positive VCA (titer 1:80) as well as EA (titer 1:40) IgA was a 48-year-old man who was referred for otorhinolaryngology (ENT) consultation. Endoscopic examination of the nasopharynx and biopsy showed undifferentiated carcinoma consistent with NPC. Another patient, a 20-year-old man who showed detectable EBV DNA (4...

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“…Although several prior studies (Zeng et al, 1982;Zeng et al, 1983;Zeng et al, 1985;Tsang et al, 2004) have demonstrated on the use of EBV serology for screening and early detection of NPC, the role of EBV DNA detection as a screening test for NPC remains inconclusive. Previous research has reported that cell-free EBV DNA can be quantitatively measured in the blood of NPC patients using polymerase chain reaction (PCR) technique Shotelersuk et al, 2000;Lin et al, 2001;Chan et al, 2003;Fan et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Systematic Review on Epstein-Barr Virus (EBV) DNA in Diagnosis of Nasopharyngeal Carcinoma in Asian Populations

Han¹,

Xu²,

Zhang³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Prospective studies on nasopharyngeal carcinoma in epstein‐barr virus IgA/VCA antibody‐positne persons in Wuzhou city, china

Cited by 171 publications

References 3 publications

Assessing the risk of nasopharyngeal carcinoma on the basis of EBV antibody spectrum

Assessing the risk of nasopharyngeal carcinoma on the basis of EBV antibody spectrum

Plasma Epstein‐Barr virus (EBV) DNA: Role as a screening test for nasopharyngeal carcinoma (NPC)?

Systematic Review on Epstein-Barr Virus (EBV) DNA in Diagnosis of Nasopharyngeal Carcinoma in Asian Populations

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