Prospective follow-up study of 877 children born after intracytoplasmic sperm injection (ICSI), with ejaculated epididymal and testicular spermatozoa and after replacement of cryopreserved embryos obtained after ICSI

Bonduelle, M; Wilikens, Ann; Buysse, Andrea; Assche, Elvire Van; Wisanto, A.; Devroey, Paul; Steirteghem, A.C. Van; Liebaers, Inge

doi:10.1093/humrep/11.suppl_4.131

Cited by 174 publications

(57 citation statements)

References 15 publications

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“…32 They found none among 29 children conceived using TESE, 1 out of 29 (3.4%) MESA and 21 of 797 (2.6%) standard ICSI. Such small numbers for the non-ejaculated sperm groups are almost meaningless in the context of congenital malformations and it has taken many years for numbers to build up at IVF centres and within registries so that comparisons can be made.…”

Section: Overall Defectsmentioning

confidence: 92%

Outcomes for offspring of men having ICSI for male factor infertility

Halliday¹

2011

Asian J Androl

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Since the introduction of intracytoplasmic sperm injection (ICSI) using single sperm isolated from testicular tissue in men with obstructive and non-obstructive azoospermia, or using ejaculated sperm in those with poor semen quality, there have been concerns that this might have adverse effects on the offspring compared to conventional in vitro fertilisation (IVF) and natural conceptions. ICSI is done for reasons other than male factor infertility, and on the whole has not been shown to have any more negative effects than those seen with IVF. There have however, been very few studies of ICSI with a focus on, or large enough numbers to examine, the specific outcomes associated with male factor infertility. From the limited information available in relation to the source of the sperm and aetiology of infertility in the presence of ICSI, there appears to be no increased risk of congenital malformations. There is, however, a small increase in both de novo and inherited chromosome abnormalities. In terms of growth and neurodevelopment, there are very few studies, and so far, no adverse outcomes have been found in young children whose fathers have a sperm defect. The origin of the sperm used in ICSI does not have a major influence on the early life outcomes for the offspring, but transgenerational and epigenetic effects remain unknown. When the male factor infertility is known or thought to be due to a Y-chromosome deletion, this information should be given to the young male offspring at a time that will ensure his own reproductive health and plans are optimized.

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Section: Overall Defectsmentioning

confidence: 92%

Outcomes for offspring of men having ICSI for male factor infertility

Halliday¹

2011

Asian J Androl

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“…If this is done then we might be in a position to finally address the question of whether increased sperm aneuploidy leads to a corresponding increase in aneuploid conceptuses. Results thus far have been encouraging (Bonduelle et al, 1996(Bonduelle et al, , 1999(Bonduelle et al, , 2002Aran et al, 2003); however, a small increase for sex chromosome abnormalities has been reported. Only through accurate assessment of sperm disomy before ICSI, followed up by rigorous clinical and cytogenetic follow-up, will the risks of sperm disomy through infertility treatment be established.…”

Section: Technical Issuesmentioning

confidence: 99%

The relationship between male infertility and increased levels of sperm disomy

Tempest

Griffin²

2004

Cytogenet Genome Res

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Sperm chromosome abnormalities cut across a number of areas relevant to ICC XV. The association between increased levels of sperm aneuploidy (usually disomy) and male infertility has implications for the sessions on reproduction, sex chromosomes, aneuploidy and meiosis and was, to the best of our knowledge, first reported in 1995. Since then most studies have reported similar increases of varying degrees but, despite this, a small number of laboratories have presented results that demonstrate no significant association. The purpose of this article is to review the state of the art in this area and to speculate as to reasons for the differences in reports from different laboratories. The findings are broken down by chromosome with studies of the sex chromosomes being broken down further to indicate meiotic stages of origin. We conclude that comparisons are difficult to make since many studies do not clearly define patient and control groups. Nevertheless, despite these and other differences (such as scoring criteria, technical differences, demographics, etc.), the consensus in the literature is that a strong correlation exists between sperm aneuploidy and male infertility. The nature of that relationship will be further defined when andrological criteria are more closely taken into account and protocols for preparation and scoring are standardised.

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“…Many aspects are still not understood. This raises concern in terms of so far undetected risks, even though follow-up data after ICSI report only a slight (but statistically relevant) increase in sex-chromosomal and de novo structural chromosome abnormalities [7,8]. There is a special fear of genetic consequences or long-term effects.…”

Section: Introductionmentioning

confidence: 99%

DNA Methylation Analysis in Immature Testicular Sperm Cells at Different Developmental Stages

Manning

Lissens²,

Weidner³

et al. 2001

Urol Int

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Testicular sperm extraction and intracytoplasmic sperm injection (TESE-ICSI) is a frequently used therapeutic option in azoospermic males. Genetic imprinting is a mechanism of gene regulation – mediated by the methylation of deoxyribonucleic acids (DNA) – by which only one of the parental copies of a gene is expressed. Whether the establishment of the genetic paternal imprint in this immature haploid sperm cell is complete and stable at this stage of maturation has never been analysed. In the present study, a highly sensitive heminested methylation-specific polymerase chain reaction for the target region 15q11–13 was developed for imprinting analysis at the single-cell level. Imprinting analysis was then carried out on DNA extracted from single diploid leucocytes (n = 25), ejaculated spermatozoa (n = 88), elongated testicular spermatids (n = 30), and round spermatids (n = 25). Amplification was obtained in 57% of the ejaculated spermatozoa, in all elongated spermatids and in 20% of the round spermatids. In the amplified samples, only the paternal imprint was detected. The maternal imprint was not found at all in any of the sperm cells; in 56% of the diploid leucocytes, the maternal and paternal imprints were detected at the same time (complete failure in the other 44%). The data reveal the completed establishment of the correct paternal imprint in ejaculated spermatozoa, elongated spermatids and amplified round spermatids. However, the high rate of amplification failure in round spermatids remains a factor of uncertainty.

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Prospective follow-up study of 877 children born after intracytoplasmic sperm injection (ICSI), with ejaculated epididymal and testicular spermatozoa and after replacement of cryopreserved embryos obtained after ICSI

Cited by 174 publications

References 15 publications

Outcomes for offspring of men having ICSI for male factor infertility

Outcomes for offspring of men having ICSI for male factor infertility

The relationship between male infertility and increased levels of sperm disomy

DNA Methylation Analysis in Immature Testicular Sperm Cells at Different Developmental Stages

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