Prospective controlled trial of an electrophoretic method of sperm preparation for assisted reproduction: comparison with density gradient centrifugation

Fleming, Steven D.; Ilad, R. S.; Griffin, AM; Wu, Yu; Ong, Kee; Smith, Heather; Aitken, R. John

doi:10.1093/humrep/den330

Cited by 79 publications

(28 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, the effectiveness of this approach has been demonstrated in case studies where conventional methods have been ineffective in isolating spermatozoa that are capable of fertilization and the initiation of normal embryonic development (Ainsworth et al, 2005(Ainsworth et al, , 2007. Recently, more extensive clinical trials have supported the practical, clinical utility of this system in the preparation of spermatozoa for ART (Fleming et al, 2008).…”

Section: Selective Isolationmentioning

confidence: 99%

Biological and clinical significance of DNA damage in the male germ line

Aitken¹,

Iuliis²,

2009

View full text Add to dashboard Cite

DNA damage is a common feature of human spermatozoa with purported links to poor rates of conception, impaired embryonic development, an increased incidence of miscarriage and the appearance of various kinds of morbidity in the offspring including childhood cancer. However, difficulties in interpretation arise, because these associations are not consistently observed across all data sets. Such inconsistency reflects the inherent complexity of the reproductive process, large variations in sample size, differences in patient selection, inadequate study design as well as inter-individual differences in the type of DNA damage being detected and the effectiveness of repair mechanisms in the oocyte. This review considers the type, source and measurement of DNA damage in human spermatozoa. It also addresses the clinical utility of the information generated in such studies, and highlights areas where further research is needed to bridge the gap between an intriguing biological phenomenon and the evidence-based clinical management of male patients characterized by high levels of DNA damage in their spermatozoa.

show abstract

Section: Selective Isolationmentioning

confidence: 99%

Biological and clinical significance of DNA damage in the male germ line

Aitken¹,

Iuliis²,

2009

View full text Add to dashboard Cite

show abstract

“…128 Comparison of density-gradient centrifugation of spermatozoa using the ISolateTM medium with the electrophoretic method revealed comparable results of sperm recovery, motility and DNA damage. 129 Recent investigations by Aitken et al 130 and Ainsworth et al 131 demonstrated that electrophoretically separated human spermatozoa are free of oxidative stress, capacitate and bind to zona pellucida normally. The technique does not result in a skewing of the ratio of X-and Y-bearing germ cells as suspected by others.…”

Section: Electrical Chargementioning

confidence: 99%

Sperm preparation: state-of-the-art—physiological aspects and application of advanced sperm preparation methods

Henkel

2011

Asian J Androl

110

View full text Add to dashboard Cite

For assisted reproduction technologies (ART), numerous techniques were developed to isolate spermatozoa capable of fertilizing oocytes. While early methodologies only focused on isolating viable, motile spermatozoa, with progress of ART, particularly intracytoplasmic sperm injection (ICSI), it became clear that these parameters are insufficient for the identification of the most suitable spermatozoon for fertilization. Conventional sperm preparation techniques, namely, swim-up, density gradient centrifugation and glass wool filtration, are not efficient enough to produce sperm populations free of DNA damage, because these techniques are not physiological and not modeled on the stringent sperm selection processes taking place in the female genital tract. These processes only allow one male germ cell out of tens of millions to fuse with the oocyte. Sites of sperm selection in the female genital tract are the cervix, uterus, uterotubal junction, oviduct, cumulus oophorus and the zona pellucida. Newer strategies of sperm preparation are founded on: (i) morphological assessment by means of 'motile sperm organelle morphological examination (MSOME)'; (ii) electrical charge; and (iii) molecular binding characteristics of the sperm cell. Whereas separation methods based on electrical charge take advantage of the sperm's adherence to a test tube surface or separate in an electrophoresis, molecular binding techniques use Annexin V or hyaluronic acid (HA) as substrates. Techniques in this category are magnet-activated cell sorting, Annexin V-activated glass wool filtration, flow cytometry and picked spermatozoa for ICSI (PICSI) from HA-coated dishes and HA-containing media. Future developments may include Raman microspectrometry, confocal light absorption and scattering spectroscopic microscopy and polarization microscopy.

show abstract

“…The study involved a couple with long-term infertility associated with extensive sperm DNA damage. Later, a prospective controlled trial was performed to demonstrate that the membrane-based electrophoresis system is as effective as and considerably faster than the DGC to prepare spermatozoa for both IVF and ICSI (Fleming et al, 2008). …”

Section: Electrophoretic Systemmentioning

confidence: 99%