Prospective, blinded comparison of cytology and DNA-image cytometry of brush biopsies for early detection of oral malignancy

Kämmerer, Peer W.; Koch, Felix P.; Santoro, Massimo; Babaryka, Gregor; Biesterfeld, Stefan; Brieger, Jürgen; Kunkel, Martin

doi:10.1016/j.oraloncology.2012.12.006

Cited by 41 publications

(42 citation statements)

References 43 publications

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“…LBC and its application on oral smears collected using a cytobrush have shown significant improvement allowing the detection of abnormal epithelial cells . Several studies have demonstrated the feasibility of using immunocytochemical staining and molecular testings using oral LBC samples . For example, Kujan et al displayed high‐quality immunoreactivity for fragile histidine triad gene (FHIT) of oral epithelial cells from healthy subjects based on LBC slides.…”

Section: Discussionmentioning

confidence: 99%

The utility of oral brush cytology in the early detection of oral cancer and oral potentially malignant disorders: A systematic review

Alsarraf

Kujan

Farah

2017

J Oral Pathology Medicine

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Findings from this study indicate that meaningful evidence-based recommendations for the implementation of a minimally invasive technique to be utilized as an adjunctive tool for screening and early detection of oral cancer and OPMDs are complicated from the reported studies in the literature. There is need for well-designed clinical studies to assess the accuracy of oral brush cytology utilizing validated cytological assessment criteria for the diagnosis and prediction of OPMDs.

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Section: Discussionmentioning

confidence: 99%

The utility of oral brush cytology in the early detection of oral cancer and oral potentially malignant disorders: A systematic review

Alsarraf

Kujan

Farah

2017

J Oral Pathology Medicine

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“…Exclusion of lesions with typical signs of oral cancer from this study is important to avoid bias of accuracy for identification of OED as conducted in similar studies . In 41% of study participants, lichen planus was diagnosed, 23% showed leukoplakia, and 3% erythroplakia.…”

Section: Discussionmentioning

confidence: 99%

“…The risk to miss oral cancer by incisional biopsy is 7‐10% in clinical studies . But risk of sampling error exists also in minimal invasive brush biopsy in combination with DNA polyploidy status …”

Section: Introductionmentioning

confidence: 99%

Advances toward fully automated in vivo assessment of oral epithelial dysplasia by nuclear endomicroscopy—A pilot study

Liese

Winter

Glass

et al. 2017

J Oral Pathology Medicine

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“…It is a quick, simple, relatively inexpensive, and non-invasive method (20). A cytobrush is employed for sample collection, as it permits easy sampling and ensures the quality of the sample (21).…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of <i>Candida albicans</i> in oral exfoliative cytology samples by loop-mediated isothermal amplification

Noguchi

Igarashi

Omagari

et al. 2017

Journal of Oral Science

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Loop-mediated isothermal amplification(LAMP) rapidly amplifies DNA under isothermal conditions. The aim of this study was to detect Candida albicans and compare the positivity rate in the LAMP reaction with that of conventional methods for oral exfoliative cytology (EC) samples. Sixty-eight EC samples from 53 patients were subjected to LAMP analysis. These patients had been clinically diagnosed with leukoplakia, squamous cell carcinoma, oral lichen planus (OLP), stomatitis, oral candidiasis, and other malignancies. LAMP reactions were defined as positive when the sample turbidity exceeded 0.1 (arbitrary unit). Periodic acid-Schiff (PAS) staining and microbial culture were also performed to detect Candida species in EC samples. The LAMP reaction detected C. albicans in 42.6% of EC samples. Candida species were detected in 32.4% of the same samples by culturing and in 29.4% of samples by PAS staining. C. albicans DNA was detected most frequently in samples from OLP patients. We conclude that, in comparison to conventional methods for detection of C. albicans, the LAMP method is highly sensitive and timesaving, and does not require expensive equipment or diagnostic technology. It may therefore be useful for on-site screening of C. albicans at dental clinics.

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Prospective, blinded comparison of cytology and DNA-image cytometry of brush biopsies for early detection of oral malignancy

Cited by 41 publications

References 43 publications

The utility of oral brush cytology in the early detection of oral cancer and oral potentially malignant disorders: A systematic review

The utility of oral brush cytology in the early detection of oral cancer and oral potentially malignant disorders: A systematic review

Advances toward fully automated in vivo assessment of oral epithelial dysplasia by nuclear endomicroscopy—A pilot study

Rapid detection of <i>Candida albicans</i> in oral exfoliative cytology samples by loop-mediated isothermal amplification

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