Prospective Advances in Circular RNA Investigation

Sulaiman, Siti Aishah; Murad, Nor Azian Abdul; Hanif, Ezanee Azlina Mohamad; Abu, Nadiah; Jamal, A. Rahman A.

doi:10.1007/978-981-13-1426-1_28

Cited by 17 publications

(13 citation statements)

References 88 publications

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“…circRNAs are critical regulators that serve an imperative role in gene expression (14,27). circRNA biomarkers and circRNA-targeted therapy have attracted increasing attention in recent years (28,29).…”

Section: Discussionmentioning

confidence: 99%

Circular RNA UBAP2 promotes the proliferation of prostate cancer cells via the miR‑1244/MAP3K2 axis

Azhati

Wang

et al. 2021

Oncol Lett

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Prostate cancer (PCa) is a common male malignant disease with a high incidence, which can seriously affect the quality of life of patients. The survival rate of patients with PCa has improved to 98.6%; however, new insights for the molecular mechanism are still urgently required. Circular RNA (circ)UBAP2 is a tumor-associated circRNA that has been demonstrated to promote the progression of various types of cancer. CircUBAP2 has been demonstrated to be significantly upregulated in PCa, but its role in the progression of PCa remains unclear. The present study aimed to provide an improved understanding of the regulatory mechanism of circUBAP2 in PCa. circUBAP2 expression was identified to be upregulated in four PCa cell lines and clinical tissues by using reverse transcription-quantitative PCR analysis. Binding sites analysis and luciferase reporter gene assay indicated that the microRNA(miR)-1244/MAP3K2 axis was the target of circUBAP2. Gain-of-function assays revealed that circUBAP2 promoted the proliferation of PCa cells by sponging miR-1244 and promoting the MAP3K2 axis. The present findings may be essential for providing new strategies in the diagnosis and targeted therapy of PCa.

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Section: Discussionmentioning

confidence: 99%

Circular RNA UBAP2 promotes the proliferation of prostate cancer cells via the miR‑1244/MAP3K2 axis

Azhati

Wang

et al. 2021

Oncol Lett

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“…A back-splicing event during the transcription process that ligates a splice donor site at the upstream to an acceptor sites at downstream of the RNA transcript, produces a covalently closed loop RNA product that does not contain either 5' to 3' polarity nor a poly-adenylated tail, and this is known as the circular RNA (circRNA) (Cao et al, 2018;Fang et al, 2018;Sulaiman et al, 2018;Hu et al, 2019). Due to its circular structure, circRNA is very stable and resistant to degradation by exoribonucleases (Liu et al, 2017;Cao et al, 2018).…”

Section: Circular Rnas (Circrnas)mentioning

confidence: 99%

Interactions Among Non-Coding RNAs in Diabetic Nephropathy

et al. 2020

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Diabetic Nephropathy (DN) is the most common cause of End-stage renal disease (ESRD). Although various treatments and diagnosis applications are available, DN remains a clinical and economic burden. Recent findings showed that noncoding RNAs (ncRNAs) play an important role in DN progression, potentially can be used as biomarkers and therapeutic targets. NcRNAs refers to the RNA species that do not encode for any protein, and the most known ncRNAs are the microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and circular RNAs (circRNAs). Dysregulation of these ncRNAs was reported before in DN patients and animal models of DN. Importantly, there are some interactions between these ncRNAs to regulate the crucial steps in DN progression. Here, we aimed to discuss the reported ncRNAs in DN and their interactions with critical genes in DN progression. Elucidating these ncRNAs regulatory network will allow for a better understanding of the molecular mechanisms in DN and how they can act as new biomarkers for DN and also as the potential targets for treatment.

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“…CircRNAs are initially found in RNA virus in 1976 and later in eukaryotes ( 14 , 15 ). They are formed by the covalent ligating of the 5′ and 3′ ends of exon(s) through back-splicing during pre-RNA splicing ( 16 ).…”

Section: Discussionmentioning

confidence: 99%

Circular RNA Protein Tyrosine Kinase 2 Promotes Cell Proliferation, Migration and Suppresses Apoptosis via Activating MicroRNA-638 Mediated MEK/ERK, WNT/β-Catenin Signaling Pathways in Multiple Myeloma

et al. 2021

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Our previous study observed that circular RNA protein tyrosine kinase 2 (circ-PTK2) was upregulated and correlated with worse clinical features and unfavorable prognosis in multiple myeloma (MM) patients. Thus, this study aimed to further characterize the regulatory function of circ-PTK2 on cell malignant activities and its target microRNA-638 (miR-638) as well as downstream MEK/ERK, WNT/β-catenin signaling pathways in MM. The effect of circ-PTK2 on MM cell proliferation, apoptosis, migration, invasion and its potential target miRNAs was assessed by transfecting circ-PTK2 overexpression plasmids into U226 cells and circ-PTK2 knock-down plasmids into LP-1 cells. Furthermore, the interaction between circ-PTK2 and miR-638 mediated MEK/ERK and WNT/β-catenin signaling pathways was validated by rescue experiments. Circ-PTK2 was overexpressed in most MM cell lines compared to normal plasma cells. Overexpressing circ-PTK2 promoted proliferation and migration, inhibited apoptosis in U266 cells, but did not affect cell invasion; knocking down circ-PTK2 achieved opposite effect in LP-1 cells. Besides, circ-PTK2 reversely regulated miR-638 expression but not miR-4690, miR-6724, miR-6749 or miR-6775. The following luciferase reporter assay illustrated the direct bind of circ-PTK2 towards miR-638. In rescue experiments, overexpressing miR-638 suppressed proliferation, migration, while promoted apoptosis in both wild U266 cells and circ-PTK2-overexpressed U266 cells; meanwhile, overexpressing miR-638 also suppressed MEK/ERK and WNT/β-catenin pathways in both wild U266 cells and circ-PTK2-overexpressed U266 cells. Knocking down miR-638 achieved opposite effect in both wild LP-1 cells and circ-PTK2-knocked-down LP-1 cells. In conclusion, circ-PTK2 promotes cell proliferation, migration, suppresses cell apoptosis via miR-638 mediated MEK&ERK and WNT&β-catenin signaling pathways in MM.

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Prospective Advances in Circular RNA Investigation

Cited by 17 publications

References 88 publications

Circular RNA UBAP2 promotes the proliferation of prostate cancer cells via the miR‑1244/MAP3K2 axis

Circular RNA UBAP2 promotes the proliferation of prostate cancer cells via the miR‑1244/MAP3K2 axis

Interactions Among Non-Coding RNAs in Diabetic Nephropathy

Circular RNA Protein Tyrosine Kinase 2 Promotes Cell Proliferation, Migration and Suppresses Apoptosis via Activating MicroRNA-638 Mediated MEK/ERK, WNT/β-Catenin Signaling Pathways in Multiple Myeloma

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