PROSPECTION OF FILAMENTOUS FUNGI AND THE PRODUCTION OF AMYLASE BY ASPERGILLUS sp. M1.7.2

Haloalkalitolerant fungi can grow at environments had high salt concentrations and pH values. The bioactive compounds produced under stressful conditions have potential biotechnological applications. In this study, 52 microfungi isolated from Acıgöl Lake/Turkey, offering polyextreme conditions, were screened for some biotechnological properties. The antimicrobial and antioxidant activities were determined using the agar diffusion and the DPPH radical scavenging method, respectively. Starch-iodine method, tributyrin plate assay, skim milk plate assay, carboxymethyl cellulose plate method were used for determining amylolytic, lipolytic, proteolytic and cellulolytic activity, respectively. Synthesis of silver nanoparticle was carried out using cell-free filtrate of microfungi. As a result, 40% of the isolates showed antimicrobial activity against at least one of the test microorganisms. Penicillium dipodomyicola showed the highest antibacterial activity against Gram positive and negative test bacteria, while P. brevicompactum showed the highest activity against Candida albicans. P. dipodomyicola and P. bilaiae were found to have free radical scavenging activity of a level (90% and above) that can compete with positive control. All of the isolates with amylase activity belonged to Aspergillus and Penicillium genera and the most prominent three of them were A. ochraceous, P. brevicompactum and A. flavus. While 55% of the isolates showed proteolytic activity, A. alliaceus had the highest activity. Almost all the isolates (92%) showed lipolytic activity. It was determined that especially the isolates of A. amstelodami, P. sizovae and P. solitum had a significant level of lipolytic activity. 35% of the isolates showed cellulolytic activity and in isolates of Cladosporium pseudocladosporioides, P. dipodomyicola and P. bilaiae were observed high activity. Eight of the isolates carried out AgNP synthesis within 24 h. When all the results were evaluated, it was determined that the isolates such as Aspergillus amstelodami, A. ochraceus, Penicillium dipodomyicola, and P. brevicompactum, have the potential to serve in different industrial areas.

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Screening of Microfungi Isolated From Acigöl, Türki̇ye for Hydrolytic Enzymes, Bioactive Metabolites and Silver Nanoparticle Production

İrdem

İlhan

ÖZBİÇEN

et al. 2023

Trakya University Journal of Natural Sciences

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Analysis of the Lipolytic Potential of Filamentous Fungi Isolated from Some Plants and Soil Samples in Minas Gerais, Brazil

Spencer¹,

Costa²,

Souza³

et al. 2020

ABB

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Microorganisms have the ability to produce several metabolites, which are widely used in biotechnological processes, including the biological catalysts called enzymes. Among these enzymes, lipases are favored because they perform various catalytic reactions such as hydrolysis, esterification, interesterification and transesterification. This work sought to isolate filamentous fungi from samples collected in Diamantina, Minas Gerais, Brazil, analyze their macroscopic morphological characteristics, determine the effect of temperature on their growth, and verify which organisms are potential lipase producers. From four collection sites, nine fungi were isolated from the leaves and soil of the jabuticaba (Plinia cauliflora) tree and three fungi from the Andu bean leaves (Cajanus cajan).

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Otimização das condições de cultivo do fungo filamentoso Fusarium sp. EA 1.3.7 para a produção de xilanases

et al. 2021

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As xilanases são enzimas muito utilizadas em processos industriais, como no branqueamento de papel, indústria de panificação, clarificação de sucos e cerveja, maltagem e na produção de biocombustíveis, devido aos inúmeros benefícios e vantagens que o catalisador biológico apresenta. Nesse contexto, objetivou-se bioprospectar fungos filamentosos, selecionar um fungo potencial produtor de xilanases, otimizar a produção enzimática e realizar a caracterização bioquímica da enzima. Das onze morfoespécies de fungos filamentosos isolados a partir de três amostras de materiais orgânicos coletadas no solo da cidade de Diamantina, Minas Gerais, Brasil, selecionou-se o Fusarium sp. EA 1.3.7 como fungo potencial xilanolítico. As condições de cultivo do microrganismo foram padronizadas em meio submerso CP, durante seis dias, a 30ºC, utilizando como fonte de nitrogênio a peptona, e o farelo de trigo como fonte de carbono; as xilanases produzidas apresentaram maiores atividades a 52ºC e pH 5,3.

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PROSPECTION OF FILAMENTOUS FUNGI AND THE PRODUCTION OF AMYLASE BY ASPERGILLUS sp. M1.7.2

Cited by 3 publications

References 21 publications

Screening of Microfungi Isolated From Acigöl, Türki̇ye for Hydrolytic Enzymes, Bioactive Metabolites and Silver Nanoparticle Production

Screening of Microfungi Isolated From Acigöl, Türki̇ye for Hydrolytic Enzymes, Bioactive Metabolites and Silver Nanoparticle Production

Analysis of the Lipolytic Potential of Filamentous Fungi Isolated from Some Plants and Soil Samples in Minas Gerais, Brazil

Otimização das condições de cultivo do fungo filamentoso Fusarium sp. EA 1.3.7 para a produção de xilanases

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