ProspectingRussula senecis: a delicacy among the tribes of West Bengal

Abstract

Russula senecis, a worldwide distributed mushroom, is exclusively popular among the tribal communities of West Bengal for food purposes. The present study focuses on its reliable taxonomic identification through macro- and micro-morphological features, DNA barcoding, confirmation of its systematic placement by phylogenetic analyses, myco-chemicals and functional activities. For the first time, the complete Internal Transcribed Spacer region of R. senecis has been sequenced and its taxonomic position within subsection Foetentinae under series Ingratae of the subgen. Ingratula is confirmed through phylogenetic analysis. For exploration of its medicinal properties, dried basidiocarps were subjected for preparation of a heat stable phenol rich extract (RusePre) using water and ethanol as solvent system. The antioxidant activity was evaluated through hydroxyl radical scavenging (EC50 5 µg/ml), chelating ability of ferrous ion (EC50 0.158 mg/ml), DPPH radical scavenging (EC50 1.34 mg/ml), reducing power (EC50 2.495 mg/ml) and total antioxidant activity methods (13.44 µg ascorbic acid equivalent/mg of extract). RusePre exhibited antimicrobial potentiality against Listeria monocytogenes, Bacillus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus. Furthermore, different parameters were tested to investigate its chemical composition, which revealed the presence of appreciable quantity of phenolic compounds, along with carotenoids and ascorbic acid. HPLC-UV fingerprint indicated the probable existence of at least 13 phenolics, of which 10 were identified (pyrogallol > kaempferol > quercetin > chlorogenic acid > ferulic acid, cinnamic acid > vanillic acid > salicylic acid > p-coumaric acid > gallic acid). Result from the present work suggests that the fraction, RusePre, may open novel prospect as a functional ingredient in antioxidant supplements and in drugs to treat infectious disease.

Water Soluble Antioxidative Crude Polysaccharide From Russula senecis Elicits TLR Modulated NF-κB Signaling Pathway and Pro-inflammatory Response in Murine Macrophages

“…One such less explored taxon is Russula senecis that has recently been documented as a new member to macrofungal flora of West Bengal, India. Interestingly, the specimen also emerged as a seasonal health promoting food among local indigenous people indicating safety for human consumption ( Khatua et al, 2015 ). Recently, medicinal activity of polysaccharide from the mushroom has been established in our previous study, although 10% NaOH solution was used as an extractant solvent ( Khatua and Acharya, 2017 ).…”

In vitro Antiradical Activities of Trametes elegans Collected from Osengere, Ibadan, Nigeria

“…3). Several authors have reported the hydroxyl radical scavenging effect of various mushrooms (Oyetayo, 2009;Khatua et al, 2015). Hydroxyl radical is an extremely reactive oxygen species that can propagate a chain reaction, wreaking havoc on many biological molecules such as DNA and lipids (Uttara et al, 2009;Kumar et al, 2011).…”

Biochemical basis of improvement of defense in tomato plant against Fusarium wilt by CaCl2

“…The mobile phase consisted of eluent A (acetonitrile) and eluent B (aqueous phosphoric acid solution, 0.1% v/v). A gradient program was used for elution: 0-2 min, 5% A; 2-5 min, 15% A; 5-10 min, 40% A; 10-15 min, 60% A; 15-18 min, 90% A (Khatua et al 2015). Gallic acid, p-coumaric acid, myricetin, caffeic acid, vanillic acid, ferulic acid, quercetin, cinnamic acid and pyrogallol (M.P.…”

“…Biomedicals. USA) were used as standards according to Khatua et al (2015). The DAAD detection was conducted at 278 nm for the quantification (Chandra et al 2014a).…”