Prospect, isolation, and characterization of microorganisms for potential use in cases of oil bioremediation along the coast of Trindade Island, Brazil

Abstract

In the present study, acrylic coupons with a thin layer of oil on the surface were incubated in the coastal water of Trindade Island, Brazil, for 60 days. The microorganisms adhered to the coupons were isolated using enrichment medium with hexadecane and naphthalene as the sole carbon and energy source. A total of 15 bacterial isolates were obtained, and the ability of these isolates to use different hydrocarbons as the source of carbon and energy was investigated. None of the isolates produced biosurfactants under our experimental conditions. Subsequently, identification methods such as partial sequencing of the 16S rRNA gene and analysis of fatty acids (MIDI) profile were employed. Among the 15 isolates, representatives of Actinobacteria, Firmicutes, and Alphaproteobacteria were detected. The isolates Rhodococcus rhodochrous TRN7 and Nocardia farcinica TRH1 were able to use all the hydrocarbons added to the culture medium (toluene, octane, xylene, naphthalene, phenanthrene, pyrene, hexadecane, anthracene, eicosane, tetracosane, triacontane, and pentacontane). Polymerase chain reaction amplification of the DNA isolated by employing primers for catechol 2,3-dioxygenase, alkane dehydrogenase and the alpha subunit of hydroxylating dioxygenases polycyclic aromatic hydrocarbon rings genes demonstrated that various isolates capable of utilizing hydrocarbons do not exhibit genes of known routes of catabolism, suggesting the existence of unknown catabolic pathways in these microorganisms. Our findings suggest that the microbiota associated to the coast of tropical oceanic islands has the ability to assist in environmental regeneration in cases of accidents involving oil spills in its shore. Thus, it motivates studies to map bioremediation strategies using the autochthonous microbiota from these environments.

Draft genome of Nocardia farcinica TRH1, a linear and polycyclic aromatic hydrocarbon-degrading bacterium isolated from the coast of Trindade Island, Brazil

“…The abundance of genes involved in biodegradation pathways in the genome of N. farcinica TRH1 implies in high metabolic plasticity of this strain, what is consistent with the results obtained during the screening of bacteria for hydrocarbon biodegradation 2 . The genome sequencing data from this study will support a better understanding of the metabolism and the potential applications of N. farcinica TRH1 in biotechnological processes, as hydrocarbons and xenobiotics bioremediation.…”

Potential of Soil Bacteria as Mercury Bioremediation Agent in Traditional Gold Mining

“…This condition shows that, without the presence of pollutants in the soils, length of time and adaptability to extreme (i.e., nutrient-poor) environments from which the bacteria originate are the two factors that shape their adaptability in the media. Bac- teria originating from the local environment can easily adapt to a polluted one (Rodrigues et al, 2015)acrylic coupons with a thin layer of oil on the surface were incubated in the coastal water of Trindade Island, Brazil, for 60 days. The microorganisms adhered to the coupons were isolated using enrichment medium with hexadecane and naphthalene as the sole carbon and energy source.…”

“…In this research, large pollutant loads tended to delay the bacterial growth (i.e., started on Day 7) because bacteria needed time to adapt to the highly contaminated environment and, at the same time, prepared themselves for growth. Bacteria from the local environment can live in a polluted one for a long time, representing their ability to adapt (Rodrigues et al, 2015)acrylic coupons with a thin layer of oil on the surface were incubated in the coastal water of Trindade Island, Brazil, for 60 days. The microorganisms adhered to the coupons were isolated using enrichment medium with hexadecane and naphthalene as the sole carbon and energy source.…”

Analysis of a phenol-adapted microbial community: degradation capacity, taxonomy and metabolic description

“…; Rodrigues et al . ). Species of this genus have demonstrated capability to degrade aromatic molecules, including phenol (Engelhardt et al .…”

“…Furthermore, the genome sequence of Kocuria rhizophila indicates the presence of putative metabolic pathways for the degradation of phenolic compounds (Takarada et al 2008). Nocardia genus participates in the degradation of different hydrocarbons (Baek et al 2007;Rodrigues et al 2015). Species of this genus have demonstrated capability to degrade aromatic molecules, including phenol (Engelhardt et al 1979;Hanne et al 1993;Dabhade et al 2009;Ma et al 2010;Hristov et al 2014;Shetty and Shetty 2016).…”