Prospect for kidney bioengineering: shortcomings of the status quo

Peloso, Andréa; Katari, Ravi; Murphy, Sean V.; Zambon, João Paulo; DeFrancesco, A.; Farney, Alan C.; Rogers, Jeffrey; Stratta, Robert J.; Manzia, Tommaso Maria; Orlando, Giuseppe

doi:10.1517/14712598.2015.993376

Cited by 30 publications

(18 citation statements)

References 103 publications

(49 reference statements)

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“…At present, regenerative medicine and tissue engineering in nephrology are promising areas of study . Ongoing novel studies in kidney bioengineering include cell‐based therapy and the development of BAK .…”

Section: Discussionmentioning

confidence: 99%

“…At present, regenerative medicine and tissue engineering in nephrology are promising areas of study. 20 Ongoing novel studies in kidney bioengineering include cell-based therapy and the development of BAK. 21 Humes et al have reviewed the development of a wearable or implantable BAK to diminish morbidity and mortality in patients with acute or chronic kidney disease, and they noted a trend shifting from an extracorporeal BAK comprised of a conventional synthetic hemofilter with a renal tubule assist device to a wearable BAK combining peritoneal dialysis and a bioartificial renal epithelial cell system.…”

Section: Discussionmentioning

confidence: 99%

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Development of a chitosan‐based tissue‐engineered renal proximal tubule conduit

Chiang

Huang

et al. 2016

J Biomed Mater Res

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Renal proximal tubule cells (RPTCs) are responsible for glomerular filtration and maintenance of water/electrolyte balance. To regenerate a proximal tubule, sufficient cell numbers and normal cell function are requisite. Collagen has been routinely used as a substrate for culturing human RPTCs (HRPTCs); however, the role of biomaterials has not been thoroughly explored. In this study, RPTCs retrieved from human nephrectomy/nephroureterectomy specimens were cultivated on chitosan as a substrate in serum-free condition for up to 150 days. HRPTCs could maintain a typical epithelial morphology and the specific differentiation feature of transporting epithelia after such long-term culture. As compared with HRPTCs cultivated on collagen, those cultivated on chitosan showed more dome formation, higher Na -K ATPase expression, lower vimentin expression, and lower transepithelial electrical resistance, indicating that HRPTCs cultivated on chitosan presented better differentiation status and would be more functional with better active transportation. Thus, the current study indicates greater scope for the use of chitosan as a biomaterial for preparing a HRPTC-coated chitosan conduit, which might be useful for the scaffold design of tissue-engineered proximal tubules. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 9-20, 2018.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Development of a chitosan‐based tissue‐engineered renal proximal tubule conduit

Chiang

Huang

et al. 2016

J Biomed Mater Res

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“…Three-dimensional (3D) bioprinting is an emerging technology that facilitates the layer-by-layer precise positioning of biological materials, biochemicals and living cells, with spatial control of the placement of functional components [ 98 ]. 3D printing technology offers alternative approaches to generating organotypic scaffolds for bioengineering of organs.…”

Section: D Bioprintingmentioning

confidence: 99%

Autologous Cells for Kidney Bioengineering

et al. 2016

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Worldwide, increasing numbers of patients are developing end-stage renal disease, and at present, the only treatment options are dialysis or kidney transplantation. Dialysis is associated with increased morbidity and mortality, poor life quality and high economic costs. Transplantation is by far the better option, but there are insufficient numbers of donor kidneys available. Therefore, there is an urgent need to explore alternative approaches. In this review, we discuss how this problem could potentially be addressed by using autologous cells and appropriate scaffolds to develop ‘bioengineered’ kidneys for transplantation. In particular, we will highlight recent breakthroughs in pluripotent stem cell biology that have led to the development of autologous renal progenitor cells capable of differentiating to all renal cell types and will discuss how these cells could be combined with appropriate scaffolds to develop a bioengineered kidney.

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“…Several hurdles must be overcome before cell-on-scaffold seeding technology will allow sound repopulation of acellular scaffolds, among which the inadequate understanding of the mechanisms underlying organ development and tissue regeneration and repair, as well as of how cells interact with the ECM; the lack of adequate strategies to achieve full reendothelialization and epithelialization; the need for identifying the most appropriate cells for recellularization; proof that constructs can be implanted easily, can sustain physiologic blood pressure, and do not clot; assessment of the viability and function in vitro and in vivo of the bio-engineered construct, as well as of the immunogenicity and oncogenic potential of the organoids in question; and the need to scale up to larger animal models and for more biomimetic bioreactors [99]. Furthermore, the ideal cell type for recellularization has yet to be determined.…”

Section: Recellularization Of Renal Ecm Scaffoldsmentioning

confidence: 99%

Tissue-Engineering Approaches to Restore Kidney Function

et al. 2015

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Kidney transplantation for the treatment of chronic kidney disease has established outcome and quality of life. However, its implementation is severely limited by a chronic shortage of donor organs; consequently, most candidates remain on dialysis and on the waiting list while accruing further morbidity and mortality. Furthermore, those patients that do receive kidney transplants are committed to a life-long regimen of immunosuppressive drugs that also carry significant adverse risk profiles. The disciplines of tissue engineering and regenerative medicine have the potential to produce alternative therapies which circumvent the obstacles posed by organ shortage and immunorejection. This review paper describes some of the most promising tissue-engineering solutions currently under investigation for the treatment of acute and chronic kidney diseases. The various stem cell therapies, whole embryo transplantation, and bioengineering with ECM scaffolds are outlined and summarized.

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Prospect for kidney bioengineering: shortcomings of the status quo

Cited by 30 publications

References 103 publications

Development of a chitosan‐based tissue‐engineered renal proximal tubule conduit

Development of a chitosan‐based tissue‐engineered renal proximal tubule conduit

Autologous Cells for Kidney Bioengineering

Tissue-Engineering Approaches to Restore Kidney Function

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