Prospecção de moléculas bioativas em esponjas marinhas da espécie Amphimedon viridis: estudos celulares e moleculares.

Different Species of Marine Sponges Diverge in Osteogenic Potential When Therapeutically Applied as Natural Scaffolds for Bone Regeneration in Rats

“…However, in the present study, in addition to the lower bone and osteoid formation and the lower number of osteoblasts in AV, a fibrous capsule formation was observed around the implant area only in this group, which may indicate an attempt by the organism to isolate the biomaterial, i.e., a rejection of Amphimedon viridis scaffolds [ 39 ]. Urabayashi [ 19 ] has demonstrated, through the in vitro chemical analysis of the crude sponge extract of this species, a cytotoxic effect in human cell lines from retinal pigment epithelium and breast carcinoma, as well as a hemolytic action in rat cells, which may explain the results of the present study, since severe local and systemic inflammatory and cytotoxic responses caused by the implants may result in delayed or non-healing of the bone [ 25 ]. Therefore, despite previous research demonstrating the osteogenic potential of marine sponges in terms of both bone formation and cell stimulation, the current study shows that the benefits may also depend on the species of sponge studied and that the integration of the material with the original tissue can be significantly inferior or even harmful depending on the species, as shown here.…”

“…Characterization analyses of the scaffolds were performed before and after an incubation period, the conditions of which were determined according to the Kokubo protocol [ 19 , 20 , 21 ]. For the SEM, FTIR, XRD and EDS aanlyses, the scaffolds were incubated in simulated body fluid (SBF; pH 7.4) in a ratio of 1:10 (mass of the material (g): volume of the SBF (mL)) during a period of 21 days.…”