EXCLI Journal; 19:Doc1282; ISSN 1611-2156 2020
DOI: 10.17179/excli2020-2830
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Prosopis juliflora leave extracts induce cell death of MCF-7, HepG2, and LS-174T cancer cell lines

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Cited by 7 publications
(4 citation statements)
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“…The most promising compounds ( 10 and 13 ) were explored for cell cycle distribution to determine the intracellular mode of action of these compounds. The cells and vehicle control were treated with each pre calculated IC 50 s for 48 h, were stained with propidium iodide, and observed for cell cycle distribution by flow cytometry [ 33 ]. As shown in Figure 2 , compound 10- treated A549 cells increased the G1 and G2 phase from 32.88 ± 2.04% to 34.66 ± 2.80% and from 29.13 ± 2.31% to 33.01 ± 2.28%, respectively, but decreased the cell distribution in the S phase from 38.48 ± 3.71% to 32.32 ± 1.51%, compared to the control cells.…”
Section: Resultsmentioning
confidence: 99%
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“…The most promising compounds ( 10 and 13 ) were explored for cell cycle distribution to determine the intracellular mode of action of these compounds. The cells and vehicle control were treated with each pre calculated IC 50 s for 48 h, were stained with propidium iodide, and observed for cell cycle distribution by flow cytometry [ 33 ]. As shown in Figure 2 , compound 10- treated A549 cells increased the G1 and G2 phase from 32.88 ± 2.04% to 34.66 ± 2.80% and from 29.13 ± 2.31% to 33.01 ± 2.28%, respectively, but decreased the cell distribution in the S phase from 38.48 ± 3.71% to 32.32 ± 1.51%, compared to the control cells.…”
Section: Resultsmentioning
confidence: 99%
“…The analysis was performed according to our previously published work [ 33 ]. Details regarding the procedure are provided in the Supplementary Material .…”
Section: Methodsmentioning
confidence: 99%
“…The effect of selected compounds on cell cycle phase distribution was determined by flow cytometry using the same protocol described elsewhere [ 44 ]. Briefly, MCF-7, A549, and SKOV3 followed the same protocol displayed by Elbehairi et al [ 44 ].…”
Section: Methodsmentioning
confidence: 99%
“…The cells were rinsed once using PBS and five times using distilled water. The full procedure was conducted as described by Elbehairi and his coworkers ( Elbehairi et al, 2020 ).…”
Section: Methodsmentioning
confidence: 99%