Propulsion of liposomes using bacterial motors

Zhang, Zhenhai; Li, Zhifei; Yu, Wei; Li, Kejie; Xie, Zhihong; Shi, Zhi‐Guo

doi:10.1088/0957-4484/24/18/185103

Cited by 26 publications

(23 citation statements)

References 21 publications

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“…Some of the most significant achievements concerning vesiclebased protocells include the incorporation of basic forms of replication 1 , communication 2 , transport 3 , biomolecule synthesis 4 , protein-protein interaction networks 5 and metabolism, amongst others [6][7][8][9][10][11] . There have also been significant advances in developing chemical and biological microreactors, either in vesicle or droplet form, that contain simple chemical reactions [12][13][14][15] .…”

mentioning

confidence: 99%

Vesicle-based artificial cells as chemical microreactors with spatially segregated reaction pathways

2014

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In the discipline of bottom-up synthetic biology, vesicles define the boundaries of artificial cells and are increasingly being used as biochemical microreactors operating in physiological environments. As the field matures, there is a need to compartmentalize processes in different spatial localities within vesicles, and for these processes to interact with one another. Here we address this by designing and constructing multi-compartment vesicles within which an engineered multi-step enzymatic pathway is carried out. The individual steps are isolated in distinct compartments, and their products traverse into adjacent compartments with the aid of transmembrane protein pores, initiating subsequent steps. Thus, an engineered signalling cascade is recreated in an artificial cellular system. Importantly, by allowing different steps of a chemical pathway to be separated in space, this platform bridges the gap between table-top chemistry and chemistry that is performed within vesicles.

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confidence: 99%

Vesicle-based artificial cells as chemical microreactors with spatially segregated reaction pathways

2014

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“…However due to the lack of methods to generate asymmetric GUVs, only the bending rigidities of symmetric GUVs have been studied. Instead, due to its high encapsulation efficiency and the ability to exert control over vesicle size, lamellarity, 34 and compartmentalization, 35,36 it is increasingly being used in bottom-up synthetic biology, [37][38][39][40][41][42] where it has been credited as one of the key technical developments responsible for the rapid expansion of this research area. 1 Unlocking the link between membrane asymmetry and membrane mechanical properties therefore has the potential to transform our understanding of membrane structure, protein stability, protein folding, protein binding and protein activity.…”

mentioning

confidence: 99%

Measurements of the effect of membrane asymmetry on the mechanical properties of lipid bilayers

et al. 2015

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We detail an approach for constructing asymmetric membranes and characterising their mechanical properties, leading to the first measurement of the effect of asymmetry on lipid bilayer mechanics. Our results demonstrate that asymmetry induces a significant increase in rigidity compared to symmetric membranes. Given that all biological membranes are asymmetric our findings have profound implications for the role of this phenomenon in biology.

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“…Tybrandt et al (2009). and mobility (Zhang et al, 2013). Furthermore, there have been considerable efforts to use synthetic vesicles and liposomes to create synthetic minimal cells that contain the minimal genetic makeup to form stable cell lines (Stano and Luisi, 2013).…”

Section: Measurement and Control Of Single Cellsmentioning

confidence: 99%

The Potential for Convergence between Synthetic Biology and Bioelectronics

2018

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The fields of synthetic biology, which focuses on genetic and cellular substrates, and bioelectronics, which focuses on interfacing electronics with biology, may appear to have little in common on the surface. However, we contend that there is potential for convergence between the two fields based on shared and complementary design principles from each field. We provide examples where this convergence is beginning to take place in the engineered measurement and control of cell populations, individual cells, and membrane transport. We propose that as the convergence spreads, bioelectronics will enable real-time sensing and control of synthetic biological processes through integration with conventional electronics. The increased capabilities resulting from this convergence may broaden the scope and deepen the impact of both synthetic biology and bioelectronics.

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Propulsion of liposomes using bacterial motors

Cited by 26 publications

References 21 publications

Vesicle-based artificial cells as chemical microreactors with spatially segregated reaction pathways

Vesicle-based artificial cells as chemical microreactors with spatially segregated reaction pathways

Measurements of the effect of membrane asymmetry on the mechanical properties of lipid bilayers

The Potential for Convergence between Synthetic Biology and Bioelectronics

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