Propuesta para seleccionar aceites esenciales de plantas de Colombia para investigación con base en su citotoxicidad

Velandia, Sindi Alejandra; Flechas, María Camila; Stashenko, Elena E.; Jimenez, Raquel Elvira Ocazionez

doi:10.17533/udea.vitae.v23n1a03

Cited by 3 publications

(1 citation statement)

References 25 publications

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“…28 Although there are no reports in humans, the S. aratocensis essential oil has demonstrated cytotoxicity to mycobacteria. 29 The essential oil from C. odorata , a related species to C. pellia , resulted toxic in human breast and cervical cancer and embryonic kidney cell lines, 30 whereas the flavonoids and steroids from C. hirsute and C. squalida species show antimicrobial activity. 31 The synonymous genus Eupatorium has extensively demonstrated antimicrobial properties.…”

Section: Discussionmentioning

confidence: 99%

In vitro Safety Assessment of Extracts and Compounds From Plants as Sunscreen Ingredients

Flórez González,

Stashenko,

Ocazionez

et al. 2024

Int J Toxicol

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This work investigated the safety of extracts obtained from plants growing in Colombia, which have previously shown UV-filter/antigenotoxic properties. The compounds in plant extracts obtained by the supercritical fluid (CO2) extraction method were identified using gas chromatography coupled to mass spectrometry (GC/MS) analysis. Cytotoxicity measured as cytotoxic concentration 50% (CC50) and genotoxicity of the plant extracts and some compounds were studied in human fibroblasts using the trypan blue exclusion assay and the Comet assay, respectively. The extracts from Pipper eriopodon and Salvia aratocensis species and the compound trans-β-caryophyllene were clearly cytotoxic to human fibroblasts. Conversely, Achyrocline satureioides, Chromolaena pellia, and Lippia origanoides extracts were relatively less cytotoxic with CC50 values of 173, 184, and 89 μg/mL, respectively. The C. pellia and L. origanoides extracts produced some degree of DNA breaks at cytotoxic concentrations. The cytotoxicity of the studied compounds was as follows, with lower CC50 values representing the most cytotoxic compounds: resveratrol (91 μM) > pinocembrin (144 μM) > quercetin (222 μM) > titanium dioxide (704 μM). Quercetin was unique among the compounds assayed in being genotoxic to human fibroblasts. Our work indicates that phytochemicals can be cytotoxic and genotoxic, demonstrating the need to establish safe concentrations of these extracts for their potential use in cosmetics.

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Section: Discussionmentioning

confidence: 99%

In vitro Safety Assessment of Extracts and Compounds From Plants as Sunscreen Ingredients

Flórez González,

Stashenko,

Ocazionez

et al. 2024

Int J Toxicol

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Study of Essential Oils from Leaves of Asteraceae Family Species Ageratina dendroides and Gynoxys verrucosa

Valarezo

Aguilera-Sarmiento

Meneses

et al. 2021

Journal of Essential Oil Bearing Plants

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Actividad antiproliferativa de aceites esenciales de plantas cultivadas en Colombia

et al. 2018

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RESUMENColombia posee gran diversidad de plantas medicinales, pero pocas han sido objeto de investigación. En este trabajo se evaluó la actividad antiproliferativa de aceites esenciales obtenidos por hidrodestilación asistida por microondas. Se analizaron 15 muestras de 11 especies en ensayos del MTT en células cancerosas MCF-7, HeLa y HepG-2 y se incluyeron células normales humanas (HEK293) y de animales (Vero y BF16F10) para evaluar selectividad. La composición química de muestras activas se determinó por cromatografía de gases acoplada a espectrometría de masas (GC-MS). Aceites esenciales de cuatro especies mostraron actividad antiproliferativa (CI 50 : 50 μg/mL) en células HeLa y/o MCF-7, en el siguiente rango (índice de selectividad en paréntesis): Piper cumanense H.B.K. (4,7) > Piper subflavum var. espejuelanum C.DC (3,9) > Salvia officinalis L. (3,6) > Piper eriopodom (Miq.) C. DC. (3,1). Ninguna muestra fue activa en células HepG-2. El análisis por CG-MS identificó β-cariofileno, α-copaeno, β-pineno, α-pineno y linalol como componentes mayoritarios. Los aceites esenciales activos pueden ser puntos de partida para desarrollo de medicamentos herbales para cuidado paliativo del cáncer.Palabras clave: aceite esencial, apoptosis, cáncer, fitoterapia, MTT. ABSTRACTColombia has a great diversity of medicinal plants, but few have been investigated. In this work, the antiproliferative activity of essential oils obtained by microwave-assisted hydrodistillation was evaluated. Fifteen samples from 11 species were analyzed in MTT assays in cancer cells MCF-7, HeLa and HepG-2, and normal human cells (HEK293) and animal cells (Vero and B16F10) were included to evaluate selectivity. The chemical composition of the active oils was determined by gas chromatography coupled to mass spectrometry (GC-MS). Essential oils from four species showed antiproliferative activity (IC 50 : <50 μg / mL) in HeLa and / or MCF-7 cells, in the following range (selectivity index in parenthesis): Piper cumanense H.B.K. (4.7) > Piper subflavum var. espejuelanum C. DC (3.9) > Salvia officinalis Linneo. (3.6) > Piper eriopodom (Miq.) C.DC. (31). The CG-MS analysis identified β-caryophyllene, α-copaene, β-pinene, α-pinene and linalool as major components. Active essential oils could serve as starting points to develop herbal medicines for cancer palliative care.

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Propuesta para seleccionar aceites esenciales de plantas de Colombia para investigación con base en su citotoxicidad

Cited by 3 publications

References 25 publications

In vitro Safety Assessment of Extracts and Compounds From Plants as Sunscreen Ingredients

In vitro Safety Assessment of Extracts and Compounds From Plants as Sunscreen Ingredients

Study of Essential Oils from Leaves of Asteraceae Family Species Ageratina dendroides and Gynoxys verrucosa

Actividad antiproliferativa de aceites esenciales de plantas cultivadas en Colombia

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