Propuesta de un panel MIRU-VNTR 5 para el tamizaje de aislamientos clínicos de Mycobacterium tuberculosis en México

Bolado-Martínez, Enrique; Candia-Plata, María del Carmen; Zenteno-Cuevas, Roberto; Damián, Fabiola Mendoza; Avilés-Acosta, Magali; Álvarez-Hernández, Gerardo

doi:10.1016/j.eimc.2014.10.010

Cited by 1 publication

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“…But to better understand the practical utility and added value of increased discriminatory power associated with the additional 12 loci in genotyping and TB transmission, it must be verified by epidemiologic investigations in different settings [43] . Several studies have demonstrated variability in the level of polymorphism of the MIRU-VNTR loci that can be attributed to the geographic origin of the strain and to differences between genetic lineages [22, 44, 45]. Furthermore, based on the allelic diversity, there is previous evidence suggesting that the number and combination of loci to be used to discriminate between genotypes can be adapted to the population of Mtb strains [46–48].…”

Section: Discussionmentioning

confidence: 99%

Exploring the “Latin American Mediterranean” family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela

et al. 2019

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Background The Latin American & Mediterranean (LAM) spoligotype family is one of the most successful genotype of Mycobacterium tuberculosis worldwide and particularly prevalent in South-America. Within this family, a sublineage named Region of Difference Rio (RD Rio ) was reported initially in Brazil and is characterized by a genomic deletion of about 26.3 kb. This lineage seems to show a specific adaptation to the Euro-Latin American population. In this context, we sought to evaluate the LAM family and the presence of the RD Rio genotype in samples from three Latin American countries including Paraguay, Venezuela and Argentina. To detect LAM strains reliably we applied a typing scheme using spoligotyping, 12 loci MIRU-VNTR, the Ag85C 103 SNP and the regions of difference RD Rio and RD174. IS6110-RFLP results were also used when available. Results Genotyping of 413 M. tuberculosis isolates from three Latin-American countries detected LAM (46%) and the ill-defined T clade (16%) as the most frequent families. The highest clustering rate was detected in the sample population from the city of Caracas in Venezuela. We observed considerable differences in the presence of the RD Rio lineage, with high frequency in Caracas-Venezuela (55%) and low frequency in Buenos Aires-Argentina (11%) and Paraguay (10%). The molecular markers (RD174, Ag85C 103 , MIRU02-MIRU40 signature) of the RD Rio lineage were essentially confirmed. For the LAM family, the most polymorphic loci were MIRU40, MIRU31, MIRU10, MIRU26, MIRU16 and the least polymorphic MIRU24, MIRU20, MIRU04, MIRU23. Conclusions Our results suggest a differential adaptation of LAM-sublineages in neighboring populations and that RD Rio strains spread regionally with different rates of distribution. The Ag85C SNP and RDs (RD174, RD Rio ) tested in this study can in fact facilitate molecular epidemiological studies of LAM strains in endemic settings and low-income countries. Electronic supplementary material The online version of this article (10.1186/s12866-019-1479-6) contains supplementary material, which is available to authorized users.

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