Proprietary Herbal Extract Downregulates the Gene Expression of Il-1 and #945; in HaCaT Cells: Possible Implications Against Nonscarring Alopecia

Abstract

BackgroundCurrently while, topical minoxidil and oral finasteride are the only medications approved in androgenetic alopecia (AGA), the cause oriented treatment and immunsupressive treatment are being performed in telogen effluvium (TE) and alopecia areata (AA) respectively. Considering the inflammatory factors in the pathogenesis of these three nonscarring alopecia forms, we have formulated a mixture for topical usage composed of six different herbal extracts (HE) which have already known antiinflammatory and antioxidant features.Materials and MethodsIn addition to performing the phytochemical analysis of HE, we detected the gene expression level of IL-1α, the crucial hair loss mediator, for the putative efficacy in nonscarring alopecia. Cell proliferation assay was performed by XTT reagent. After determination of non-cytotoxic concentration, HaCaT cells were treated with HE. RNA isolations were carried out from both non-treated and treated cell groups by using TRI-reagent. Gene expressions of IL-1α and as control GAPDH were determined by RT-qPCR analysis.ResultsResults were represented as “IL-1α/GAPDH Fold Change”. HE solution caused statistically significant downregulation of IL-1α gene expressions (p<0.0001), compared to untreated control cells. HE treatment ended up with 0.1900 fold change for IL-1α.ConclusionIL-1α is a direct growth inhibitory agent in hair follicles and an important actor in the pathogenesis of AGA , TE, and AA. Considering together the vitamins, flavonoids, and trace elements identified in the phytochemical analyses and downregulation of IL-1α in HaCaT cells, our HE may be an auxiliary agent in the therapy of these three nonscarring alopecia forms.

Carob Antioxidants in Human Health: From Traditional Uses to Modern Pharmacology

“…Pods aqueous extract (with 5 other plants) downregulated IL-1α in HaCaT cells, suggesting nonscarring hair loss prevention activity [24].…”

Análise comparativa entre os tratamentos para alopecia: uma revisão sistemática

“…Viu-se que o epigalocatequina-3-galato é mais seguro e mais específico que os inibidores da JAK. Pekmezci, et al, 2018. Imunoensaio clínico feito com cultura de células HaCat in vitro a partir de pele histologicamente normal. Nessas células foi usado um conjunto de extratos herbais.…”

“…As ervas usadas foram: extrato de raiz de Urtica dioica, extrato de folha de Urtica urens, extrato de folha do Equisetum arvense, extrato da parte aérea de Achillea millefolium, extrato da folha de Matricaria chamomilla e extrato do fruto de Ceratonia siliqua. O extrato foi usado em cultura de células HaCat in vitro retiradas da pele histologicamente normal, essas foram submetidas a diferentes concentrações da solução de ervas (Pekmezci, Dundar & Turkoglu, 2018).…”

“…A Achillea millefolium tem propriedades anti-inflamatórias, a Matricaria chamomilla estimula a fase catágena no ciclo de crescimento capilar (fase de transição entre a fase anágena e telógena, em que o fio ainda não se destacou do couro cabeludo) e a Ceratonia siliqua tem propriedades anti-oxidantes. Devido a todos esses benefícios citados, o extrato herbal em questão pode ser considerado um agente promissor no tratamento da alopecia (Pekmezci, Dundar & Turkoglu, 2018).…”

Application of Ultrasound as Clean Technology for Extraction of Specialized Metabolites From Stinging Nettle (Urtica dioica L.)

“…In addition, nettle extract is used as a source of natural green color (natural dye) or pigment of natural origin for coloring soaps and shampoos, cosmetics, various lotions, toothpastes, antibacterial mouthwashes, and edible fats ( 7 ). It is used for sparse and weak hair, prevents alopecia ( 31 ).…”