Propranolol stereoisomer plasma concentrations and portal haemodynamic response in patients with liver cirrhosis

Schepke, Michael; Raab, Peter; Hoppe, Alexander; Brensing, Karl August; Paar, D.; Potyka, U.; Sauerbruch, Tilman

doi:10.1046/j.1365-2036.1999.00622.x

Cited by 12 publications

(6 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…up to 15 ng/mL after oral application, clenbuterol up to 30 ng/mL, toremifene >1000 ng/mL, trenbolone 50 ng/mL (in horse plasma), exemestane up to 50 ng/mL, salbutamol 10 ng/mL after oral application, formoterol up to 20 ng/mL after inhalative administration (i.v. ), resveratrol 40 ng/mL and GW1516 in a range of 40 to 700 ng/mL 29–44. Plasma concentrations of over 5 µg/mL for the selected peptides were reported for growth hormone release peptide‐2 (GHRP‐2) after intravenous administration of 3 mg/kg (in rats) 45.…”

Section: Resultsmentioning

confidence: 99%

Comprehensive plasma‐screening for known and unknown substances in doping controls

Thomas

Guddat

Kohler

et al. 2010

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

Occasionally, doping analysis has been recognized as a competitive challenge between cheating sportsmen and the analytical capabilities of testing laboratories. Both have made immense progress during the last decades, but obviously the athletes have the questionable benefit of frequently being able to switch to new, unknown and untested compounds to enhance their performance. Thus, as analytical counteraction and for effective drug testing, a complementary approach to classical targeted methods is required in order to implement a comprehensive screening procedure for known and unknown xenobiotics. The present study provides a new analytical strategy to circumvent the targeted character of classical doping controls without losing the required sensitivity and specificity. Using 50 microL of plasma only, the method potentially identifies illicit drugs in low ng/mL concentrations. Plasma provides the biological fluid with the circulating, unmodified xenobiotics; thus the identification of unknown compounds is facilitated. After a simple protein precipitation, liquid chromatographic separation and subsequent detection by means of high resolution/high accuracy orbitrap mass spectrometry, the procedure enables the determination of numerous compounds from different classes prohibited by the World Anti-Doping Agency (WADA). A new hyphenated mass spectrometry technology was employed without precursor ion selection for higher collision energy dissociation (HCD) fragmentation experiments. Thus the mass spectra contained all the desired information to identify unknown substances retrospectively. The method was validated for 32 selected model compounds for qualitative purposes considering the parameters specificity, selectivity, limit of detection (<0.1-10 ng/mL), precision (9-28%), robustness, linearity, ion suppression and recovery (80-112%). In addition to the identification of unknown compounds, the plasma samples were simultaneously screened for known prohibited targets.

show abstract

Section: Resultsmentioning

confidence: 99%

Comprehensive plasma‐screening for known and unknown substances in doping controls

Thomas

Guddat

Kohler

et al. 2010

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

show abstract

“…When the sample point was adjusted to the center of the portal vein, the portal venous velocity was recorded during a quiet suspended expiration and was averaged over a few seconds. The PVF was determined by the formula: crosssectional area × mean velocity × 60 (20). The coefficient of variation for the PVF measurements with this method in our center is 3% (21).…”

Section: Hemodynamic Measurementsmentioning

confidence: 99%

Acute Hemodynamic Effects of Octreotide and Terlipressin in Patients with Cirrhosis: A Randomized Comparison

et al. 2005

View full text Add to dashboard Cite

show abstract

“…In patients with liver disease the concentration of the S‐propranolol is greatly elevated. Stereoisomer concentrations, however, do not appear to predict the haemodynamic response to propranolol, and there is no difference in the relative concentrations of both stereoisomers in propranolol responders and nonresponders 24 …”

Section: Haemodynamic Studiesmentioning

confidence: 88%

A drug therapy for the prevention of variceal haemorrhage

Tripathi

Hayes

2001

Aliment Pharmacol Ther

View full text Add to dashboard Cite

The development of varices is a major complication of cirrhosis, and variceal haemorrhage has a high mortality. There have been major advances in the primary and secondary prevention of variceal haemorrhage over the last 20 years involving endoscopic, radiological and pharmacological approaches. This review concentrates principally on drug therapy, particularly on the numerous haemodynamic studies. Many of these drugs have not been studied in clinical trials, but provide data about the underlying pathogenesis of portal hypertension. Also covered in this review are the randomized controlled trials and meta‐analyses that involve a large number of patients. These trials involve relatively few drugs such as non‐selective beta‐blockers and nitrates. Correlations between haemodynamic and clinical parameters are discussed. Despite the recent increase in the use of alternative endoscopic therapies, an effective and well tolerated drug remains a clinically important research goal.

show abstract

Propranolol stereoisomer plasma concentrations and portal haemodynamic response in patients with liver cirrhosis

Abstract: Our data demonstrate a stereoselective metabolism of propranolol enantiomers in liver cirrhosis. However, following oral propranolol administration, stereoisomer plasma concentrations do not predict the portal haemodynamic effect.

Cited by 12 publications

References 30 publications

Comprehensive plasma‐screening for known and unknown substances in doping controls

Comprehensive plasma‐screening for known and unknown substances in doping controls

Acute Hemodynamic Effects of Octreotide and Terlipressin in Patients with Cirrhosis: A Randomized Comparison

A drug therapy for the prevention of variceal haemorrhage

Contact Info

Product

Resources

About