Propranolol selectively inhibits cervical cancer cell growth by suppressing the cGMP/PKG pathway

Gong, Liuyun; Lei, Yutiantian; Tan, Xiaoli; Dong, Yanyan; Luo, Zhuojing; Zhang, Dan; Han, Suxia

doi:10.1016/j.biopha.2019.01.027

Cited by 33 publications

(28 citation statements)

References 25 publications

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“…Moreover, our results also suggested that endocrine system and digestive system played vital roles in cold tolerance. Especially, the cAMP and cGMP-PKG signaling pathway were all significantly enriched in the DEGs of H0 vs L0, H1 vs L1, and H2 vs L2, indicating the involvement of energy metabolism [43,44] and cell growth and differentiation [45,46]. However, unlike 0, 1 and 2 libraries, chitin metabolic process, chitin binding, structural constituent of cuticle were not obviously enriched in 3 libraries.…”

Section: Developmental Transcriptomes Comparisons Between 4°c and 20°cmentioning

confidence: 95%

Dynamic transcriptome profiling exploring cold tolerance in forensically important blow fly, Aldrichina grahami (Diptera: Calliphoridae)

et al. 2020

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Background: Aldrichina grahami (Diptera: Calliphoridae) is a forensically important fly, which has been widely applied to practical legal investigations. Unlike other necrophagous flies, A. grahami exhibits cold tolerance which helps to maintain its activity during low-temperature months, when other species are usually not active. Hence, A. grahami is considered an important forensic insect especially in cold seasons. In this study, we aim to explore the molecular mechanisms of cold tolerance of A. grahami through transcriptome. Results: We collected eggs and larvae (first-instar, second-instar and third-instar) at three different temperatures (4°C, 12°C and 20°C) and performed RNA-seq analyses. The differentially expressed genes (DEGs) associated with the cold-tolerance were screened out. The Venn analysis of DEGs from egg to third-instar larvae at three different temperatures showed there were 9 common genes. Candidate biological processes and genes were identified which refer to growth, and development of different temperatures, especially the chitin and cuticle metabolic process. The series-clusters showed crucial and unique trends when the temperature changed. Moreover, by comparing the results of growth and developmental transcriptomes from different temperatures, we found that DEGs belonging to the family of larval cuticle proteins (LCP), pupal cuticle protein (CUP), and heat shock proteins (HSP) have certain differences. Conclusions: This study identified functional genes and showed differences in the expression pattern of diverse temperatures. The DEGs series-clusters with increasing or decreasing trends were analyzed which may play an important role in cold-tolerance. Moreover, the findings in LCP, CUP and HSP showed more possible modulations in a cold environment. This work will provide valuable information for the future investigation of the molecular mechanism of cold tolerance in A. grahami.

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Section: Developmental Transcriptomes Comparisons Between 4°c and 20°cmentioning

confidence: 95%

Dynamic transcriptome profiling exploring cold tolerance in forensically important blow fly, Aldrichina grahami (Diptera: Calliphoridae)

et al. 2020

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“…Huang et al [36] reported that focal adhesion exerted traction on cells by connecting actin cytoskeleton to the extracellular matrix, which plays an important role in the invasion and metastasis of OS cells. Previous studies have reported that the calcium signaling and cGMP−PKG signaling pathways may be potential therapeutic targets for tumors [37,38]. To validate the KEGG enrichment results and further understand the pathway enrichment of the dataset, we performed GSEA and GSVA on the merged expression pro le data.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Immune Infiltration Pattern in Osteosarcoma and Its Clinical Significance

Yuan¹,

Deng²,

Ren³

et al. 2020

Preprint

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ObjectiveOsteosarcoma (OS), the most common malignant bone tumor, is prone to early metastasis and recurrence, resulting in poor prognosis. The purpose of this study was to investigate gene expression in OS samples, and to study the relationship between tumor infiltrating immune cells (TIICS) in the tumor microenvironment and the occurrence, development, and prognosis of OS. MethodsIntegrated analysis was performed on the gene expression profile of OS samples in the Therapeutically Applicable Research to Generate Effective Treatments (TARGET) database, and normal muscle tissue samples in the Genotype-Tissue Expression (GTEx) database. Screening of differentially expressed genes in OS samples and healthy muscle tissue samples was performed using R software. Functional annotation and pathway enrichment analyses were performed, a protein-protein interaction network was constructed, and the Hub genes were screened. The infiltration differences of 22 TIICs in OS samples and normal muscle tissue samples were analyzed using CIBERSORT. ROC curve, and OS survival analyses were performed on the Hub genes and 22 TIICs. The correlation between Hub genes with prognostic value and the 22 TIICs was discussed. ResultsTen Hub genes were screened. OS and normal muscle tissue samples were compared. Significant differences were observed in the infiltration of follicular helper T cells, resting NK cells, plasma cells, activated CD4 memory T cells, gamma delta T cells, monocytes, M0 macrophages and resting dendritic cells (P<0.05). ROC curve analysis showed that the area under the curve (AUC) for the 10 hub genes was 0.9 ~ 1.0, and for naive B cells (AUC=0.942), plasma cells (AUC=0.999), follicular T helper cells (AUC=0.814), resting NK cells (AUC=0.864), monocytes (AUC=0.987), and M0 macrophages (AUC=0.995). Kaplan-Meier curves revealed positive correlation between the expression levels of TNNI2, TTN, ACTN3, TNNC2, MYL3, and survival of OS patients. Correlation analysis revealed close association between ACTN3, TNNI2, TNNC2, MYL3, TTN, and the infiltration of M0 macrophages and plasma cells. ConclusionOur findings provide a theoretical basis for further study of the molecular mechanisms underlying OS development and occurrence. It serves as a reference for the development of new diagnostics, identification of prognostic biomarkers, and targeted drug therapy for OS.

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“…Even though survival rates are rising, new therapies are still needed to reduce mortality rates and increase patients' life quality. Evidence suggests that the cGMP signaling pathway is aberrantly regulated in different cancers, including breast cancer (35)(36)(37). Some cyclic nucleotides can act as second messengers promoting protein kinase activation.…”

Section: Breast Cancermentioning

confidence: 99%

New Approaches in Oncology for Repositioning Drugs: The Case of PDE5 Inhibitor Sildenafil

et al. 2021

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The use of already-approved drugs to treat new or alternative diseases has proved to be beneficial in medicine, because it reduces both drug development costs and timelines. Most drugs can be used to treat different illnesses, due their mechanisms of action are not restricted to one molecular target, organ or illness. Diverging from its original intent offers an opportunity to repurpose previously approved drugs to treat other ailments. This is the case of sildenafil (Viagra), a phosphodiesterase-5 (PDE5) inhibitor, which was originally designed to treat systemic hypertension and angina but is currently commercialized as erectile dysfunction treatment. Sildenafil, tadalafil, and vardenafil are PDE5 inhibitors and potent vasodilators, that extend the physiological effects of nitric oxide and cyclic guanosine monophosphate (cGMP) signaling. Although most of the biological implications of these signaling regulations remain unknown, they offer a large therapeutic potential for several diseases. In addition, some PDE5 inhibitors’ molecular effects seem to play a key role in different illnesses such as kidney disease, diabetes mellitus, and cancer. In this review, we discuss the molecular effects of PDE5 inhibitors and their therapeutic repurposing in different types of cancer.

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Propranolol selectively inhibits cervical cancer cell growth by suppressing the cGMP/PKG pathway

Cited by 33 publications

References 25 publications

Dynamic transcriptome profiling exploring cold tolerance in forensically important blow fly, Aldrichina grahami (Diptera: Calliphoridae)

Dynamic transcriptome profiling exploring cold tolerance in forensically important blow fly, Aldrichina grahami (Diptera: Calliphoridae)

Analysis of Immune Infiltration Pattern in Osteosarcoma and Its Clinical Significance

New Approaches in Oncology for Repositioning Drugs: The Case of PDE5 Inhibitor Sildenafil

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