Proposed panel of diagnostic tools for accurate temporal classification of symptomatic T. gondii infection

Barros, Geisa Baptista; Lemos, Elenice Moreira; Silva-dos-Santos, Priscila Pinto; Dietze, Reynaldo; Zandonade, Eliana; Mineo, José Roberto; Silva, Deise Aparecida de Oliveira; Pajuaba, Ana Cláudia Marquez; Gomes, Matheus de Souza; Amaral, Laurence Rodrigues do; Coelho-dos-Reis, Jordana Grazziela Alves; Martins‐Filho, Olindo Assis; Serufo, José Carlos

doi:10.1016/j.jim.2017.08.010

Cited by 9 publications

(9 citation statements)

References 25 publications

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“…Similarly, only 29.2% of participants knew that the demonstration of anti- T. gondii IgM antibodies without IgG in the serum of a pregnant woman is not enough reason to provide treatment. All pregnant women with a positive anti- T. gondii IgM test should be further examined with the anti- T. gondii IgG avidity test to discriminate between acute and past infections [ 19 , 20 ]. Only women with low anti- T. gondii IgG avidity should be considered for treatment [ 19 , 20 ].…”

Section: Discussionmentioning

confidence: 99%

“…All pregnant women with a positive anti- T. gondii IgM test should be further examined with the anti- T. gondii IgG avidity test to discriminate between acute and past infections [ 19 , 20 ]. Only women with low anti- T. gondii IgG avidity should be considered for treatment [ 19 , 20 ]. Poor knowledge of the avidity test found in the present study has also been reported with respect to physicians attending pregnant women in Mexico [ 25 ] and the USA [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

“…Anti- T. gondii IgM antibodies can persist for several years and a chronic T. gondii infection can be erroneously classified as an acute infection if diagnosis is based on IgM serology only [ 18 ]. Avidity tests of specific anti- T. gondii IgG antibodies are an important tool for discrimination of recent and past infections, especially in pregnant women [ 19 , 20 ]. Other serological tests for the diagnosis of T. gondii infection, i.e., Western blot [ 21 ] and indirect immunofluorescence test [ 22 ], are used less frequently.…”

Section: Introductionmentioning

confidence: 99%

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Knowledge and Practices of Toxoplasmosis among Clinical Laboratory Professionals: A Cross-Sectional Study in Durango, Mexico

Alvarado‐Esquivel

Sánchez‐Anguiano²,

Berumen-Segovia

et al. 2017

IJERPH

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Background: The aim of this study was to determine the level of knowledge and practices about toxoplasmosis in a sample of clinical laboratory professionals in Mexico. Methods: 192 clinical laboratory professionals were surveyed. They were asked about (1) Toxoplasma gondii; (2) clinical manifestations, diagnosis, treatment, and epidemiology of toxoplasmosis; and (3) their practices with respect to toxoplasmosis. Results: The range of animals infected by T. gondii was known by 44.8% of participants. Clinical aspects of toxoplasmosis were known by up to 44.3% of subjects. Correct answers about the interpretation of serological markers of T. gondii infection were provided by up to 32.8% of participants. A minority (32.2%) of participants knew about a high number of false positive results of anti-T. gondii IgM antibody tests. Most participants (90.1%) did not know what the anti-T. gondii IgG avidity test was. Up to 55.7% of participants provided incorrect answers about the interpretation of serology tests for the treatment of pregnant women. Common routes of T. gondii infection were known by <15% of participants. Most (84.4%) participants had not performed tests for detection T. gondii infection. Conclusions: Results indicate incomplete knowledge of T. gondii infection and toxoplasmosis and a limited practice of laboratory tests among the professionals surveyed.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Knowledge and Practices of Toxoplasmosis among Clinical Laboratory Professionals: A Cross-Sectional Study in Durango, Mexico

Alvarado‐Esquivel

Sánchez‐Anguiano²,

Berumen-Segovia

et al. 2017

IJERPH

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“…Among the selected studies, the use of samples such as serum, whole blood, colostrum and amniotic fluid was observed, and these were associated with different methods of detection of T. gondii and its respective markers. Table 1 presents the general characteristics of the selected articles [6][7][8][9][10][11][12][13][14][15][16][17][18] .…”

Section: General Characteristics Of the Study And Total Resultsmentioning

confidence: 99%

Molecular and serological diagnosis of toxoplasmosis: a systematic review and meta-analysis

Souza

Siqueira

Ribeiro

et al. 2023

Rev. Inst. Med. trop. S. Paulo

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Toxoplasmosis is an infection of vast worldwide distribution whose etiologic agent is Toxoplasma gondii. This disease can cause problems ranging from mild symptoms to serious conditions, such as encephalitis, miscarriage and blindness. Therefore, it is of utmost importance to perform a diagnosis with reproducible techniques in order to obtain a good prognosis. The aim of this review was to analyze the efficiency of toxoplasmosis diagnostic techniques based on sensitivity and specificity results. Five research platforms in English language were used (Eric, Elsevier, Google Scholar, PubMed and SciELO), which contained data on the diagnosis of toxoplasmosis. The search and selection were performed for studies published prior to June 2021. The search resulted in the inclusion of 13 articles published from 2005 to 2020. The data revealed the use of different samples in the standardization of techniques such as serum, total blood, colostrum and amniotic fluid. The flow cytometry, lateral flow immunoassay and qPCR techniques showed 100% sensitivity, whereas the ELISA, western blotting, qPCR and RE-LAMP techniques achieved 100% specificity. Significantly, the qPCR and LAMP techniques were more accurate when the likelihood ratio was assessed. The meta-analysis identified that ISAGA and western blotting have low sensitivity values and LIASON, ELFA and ELISA, using a silica bioconjugate, also have low specificity values. It was noted that a wide range of methods have high values of sensitivity and specificity. Therefore, the choice of the method will be based on the conditions and its financial viability.

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“…Таким образом, в ситуациях, когда терапевтические решения зависят от даты инфицирования (например, при беременности), тест на антитела с низкой авидностью не является диагностическим для острого заболевания [20]. Для более точного определения времени инфицирования предлагается использовать сочетание различных методов (скрининг -определение IgM методом РНИФ, затем подтверждающие тесты: определение IgM и IgG методом РНИФ и определение авидности IgG) [21].…”

Section: определение авидности антителunclassified

Modern Serological Diagnosis of Toxoplasmosis: Literature Review

Мицура¹

2022

Клиническая Инфектология И Паразитология

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Представлен обзор литературных источников на английском и русском языках, посвященный оценке значения серологических методов лабораторной диагностики токсоплазмоза на современном этапе. Для диагностики токсоплазмоза необходимы лабораторные исследования, поскольку клинические проявления очень разнообразны. Токсоплазмоз у иммунокомпетентных лиц можно диагностировать с помощью серологических тестов (например, иммуноферментного анализа). Выявление специфических антител к T. gondii классов IgM и IgG позволяет определить факт инфицирования, однако не всегда возможно с их помощью диагностировать острую инфекцию. Для повышения эффективности диагностики острой или недавней инфекции используют определение авидности антител IgG, исследование на антитела класса IgA. Разработаны новые методики, основанные на использовании рекомбинантных антигенов токсоплазмы, иммунохроматографические тесты, исследование слюны и др. Следует стремиться к максимально ранней диагностике острого инфицирования токсоплазмой беременных и использовать для этого все имеющиеся возможности серологической диагностики. A review of literature sources in English and Russian is presented, devoted to the assessment of the significance of serological methods for the laboratory diagnosis of toxoplasmosis at the present time. Laboratory tests are necessary to diagnose toxoplasmosis, since the clinical manifestations are very diverse. Toxoplasmosis in immunocompetent individuals can be diagnosed using serological tests (eg, enzyme linked immunosorbent assay). Detection of specific antibodies against T. gondii of the IgM and IgG classes makes it possible to determine the fact of infection, but it is not always possible to diagnose an acute infection by them. To improve the efficiency of diagnosing an acute or recent infection, the detection of the avidity of IgG antibodies, the study of antibodies of the IgA class, are used. New methods have been developed based on the use of recombinant toxoplasma antigens, immunochromatographic tests, saliva testing, and others. It is necessary to strive for the earliest possible diagnosis of acute infection with Toxoplasma in pregnant women and use all available possibilities for serological diagnosis for this.

show abstract

Proposed panel of diagnostic tools for accurate temporal classification of symptomatic T. gondii infection

Cited by 9 publications

References 25 publications

Knowledge and Practices of Toxoplasmosis among Clinical Laboratory Professionals: A Cross-Sectional Study in Durango, Mexico

Knowledge and Practices of Toxoplasmosis among Clinical Laboratory Professionals: A Cross-Sectional Study in Durango, Mexico

Molecular and serological diagnosis of toxoplasmosis: a systematic review and meta-analysis

Modern Serological Diagnosis of Toxoplasmosis: Literature Review

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