Proposed nomenclature for quantifying subdivisions of the bronchial wall

Bai, A Gowri; Eidelman, David H.; Hogg, James C.; James, Alan; Lambert, Robert A.; Ludwig, Mara S.; Martin, James G.; McDonald, D.; Mitzner, Wayne; Okazawa, Mitsushi

doi:10.1152/jappl.1994.77.2.1011

Cited by 153 publications

(82 citation statements)

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“…The thickness of the bronchial wall was scored using the Bai method. 17) A minimum of 3 fields throughout the upper and lower left lung tissue were randomly examined for the morphometric analysis. The histologic and morphometric analyses were performed by individuals blinded to the protocol design.…”

Section: Allergen Immunization and Resveratrol Treatmentmentioning

confidence: 99%

Antiasthmatic Effects of Resveratrol in Ovalbumin-Induced Asthma Model Mice Involved in the Upregulation of PTEN

Chen

Tang

et al. 2015

Biological & Pharmaceutical Bulletin

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Resveratrol, a natural polyphenolic compound known for its antioxidative and antiinflammatory effects, exerts antiasthmatic effects, although the mechanism underlying these effects remains elusive. The phosphatase and tensin homology deleted on chromosome ten gene (PTEN) is involved in the pathogenesis of asthma, and PTEN overexpression in asthmatic mice improved asthma symptoms. To investigate whether the antiasthmatic mechanisms of resveratrol correlated with the upregulation of PTEN expression, an ovalbumin (OVA)-induced murine asthma model was used to determine the effectiveness of resveratrol treatment. PTEN mRNA and protein expression was assessed with real-time polymerase chain reaction (PCR) and immunochemistry. To determine whether airway remodeling occurred, the inner airway wall, mucous layer, and smooth muscle areas were each determined using an image analysis system. The lung epithelial cell line 16HBE was used to study the regulation of PTEN expression levels by resveratrol in vitro. Our data demonstrated that resveratrol inhibited OVA-induced airway inflammation and airway remodeling in asthmatic mice. PTEN expression was decreased in the murine asthma model, although the expression of PTEN was restored following treatment with resveratrol. Correlation efficiency analysis showed that PTEN expression was associated with the degree of airway remodeling. Further in vitro studies demonstrated that the inhibition of Sirtuin 1 (SIRT1) activity by a SIRT1 inhibitor and RNA interference decreased PTEN protein expression, while resveratrol attenuated the decreases in PTEN expression induced by the SIRT1 inhibitor. These data suggest the mechanism of the antiasthmatic effects of resveratrol in an OVA-induced murine asthma model, which resulted in the upregulation of PTEN via SIRT1 activation.Key words resveratrol; asthma; phosphatase and tensin homology deleted on chromosome ten; Sirtuin 1 (SIRT1) Asthma is an inflammatory disease that has been verified in clinical practice. Corticosteroids are unique anti-inflammatory agents and represent an important therapy for controlling asthma symptoms.

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Section: Allergen Immunization and Resveratrol Treatmentmentioning

confidence: 99%

Antiasthmatic Effects of Resveratrol in Ovalbumin-Induced Asthma Model Mice Involved in the Upregulation of PTEN

Chen

Tang

et al. 2015

Biological & Pharmaceutical Bulletin

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“…Estimates of 2D mast cell density in 5-mm transverse airway sections Both the inner airway wall (WAi), from the epithelial basement membrane to the outer border of the airway smooth muscle layer, and the total airway wall (WAt), from the epithelial basement membrane to the outer border of the adventitia [35], were delineated on CAST, and, within these reference areas, samples of UR 3,084.3 mm 2 fields comprising 17% of WAi and 5% of WAt were generated. These fields were presented sequentially on the high-resolution CAST monitor and positively stained, nucleated cell profiles in focus were counted within a single focal plane with the aid of an unbiased counting frame [36].…”

Section: Cell Countingmentioning

confidence: 99%

Do bronchial biopsies represent mast cell density in airways? A stereological study

Carroll

Carroll²

2006

European Respiratory Journal

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Endobronchial biopsy specimens may not adequately represent inflammatory cell counts throughout the airway wall. The present study aimed to compare mast cell density in biopsies and airway sections using both stereological and nonstereological methods.Post mortem biopsies and adjacent transverse sections were obtained from a mean of five proximal airways per case in 10 subjects who had died of nonrespiratory causes. Tryptasepositive mast cells were measured stereologically in 30-mm sections and nonstereologically in 5-mm sections using an optical disector (cells?mm ), respectively. Reference areas included the inner and total airway wall and to 100 mm below the basement membrane.Case means, based on four or more biopsy sites, significantly correlated with those on transverse sections for counts over the inner airway wall only, using both stereological and nonstereological methods. Cells?mm -3 and cells?mm -2 were significantly correlated within all reference areas. When endobronchial biopsies are obtained from at least four proximal airways per case, intersubject comparisons of mean mast cell density in the inner airway wall are as well represented by counts on biopsies as they are on transverse sections. This is the case using either threedimensional, stereological or two-dimensional, nonstereological methods.

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“…A camera sampled the image of each airway and quantitative measurements were performed on the digital representation of the airways via a computerized image analysis system (KS400; Zeiss, Oberkochen, Germany). The morphometrical parameters 15 that were marked manually on the digital representation of the airway were as follows: Pbm, the area defined by the basement membrane (Abm), and the area defined by the total adventitial perimeter (Ao). The total bronchial wall area (WAt) was calculated from these values (WAt = Ao e Abm), and WAt was normalized to the square length of the basement membrane (WAt/Pbm 2 ).…”

Section: Histologic and Morphometric Analysismentioning

confidence: 99%

The role of the tachykinin NK1 receptor in airway changes in a mouse model of allergic asthma

Swert

Tournoy

Joos

et al. 2004

Journal of Allergy and Clinical Immunology

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Background: Tachykinins are present in sensory nerves and in nonneuronal cells like macrophages. Human data suggest a role for these peptides in asthma, but the exact role of tachykinins and their receptors in allergic airway inflammation is still a matter of debate. Objective: The aim of this study was to determine the role of the tachykinin NK 1 receptor in allergic airway responses in a mouse model. Methods: Tachykinin NK 1 receptor wild-type and knockout animals were sensitized intraperitoneally to ovalbumin and subsequently exposed from days 14 to 21 to aerosolized ovalbumin (1%). On day 22, the immunologic and histologic changes were evaluated, and lung function measurements were performed. Results: Mice lacking the tachykinin NK 1 receptor and their wild-type litter mates developed inflammatory cell infiltrates in the airways and ovalbumin-specific IgE on sensitization and exposure to ovalbumin compared with saline-exposed controls. No differences were detected between wild-type and knockout mice. The substance P content of alveolar macrophages was not influenced by ovalbumin or by the lack of the NK 1 receptor. Ovalbumin-induced hyperresponsiveness was not observed, but at baseline, the knockout mice were more reactive despite similar morphology. Ovalbumin induced more goblet cell hyperplasia in wild-type animals compared with knockout animals. No differences in airway wall thickness were observed. Conclusion: These data suggest that tachykinin NK 1 receptors do not affect allergic airway inflammation or endogenous substance P content of alveolar macrophages but influence baseline responsiveness and promote features of remodeling such as goblet cell

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Proposed nomenclature for quantifying subdivisions of the bronchial wall

Cited by 153 publications

References 0 publications

Antiasthmatic Effects of Resveratrol in Ovalbumin-Induced Asthma Model Mice Involved in the Upregulation of PTEN

Antiasthmatic Effects of Resveratrol in Ovalbumin-Induced Asthma Model Mice Involved in the Upregulation of PTEN

Do bronchial biopsies represent mast cell density in airways? A stereological study

The role of the tachykinin NK1 receptor in airway changes in a mouse model of allergic asthma

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