Proposed model for in vitro interaction between fenitrothion and DNA, by using competitive fluorescence, 31P NMR, 1H NMR, FT-IR, CD and molecular modeling

Studying non-covalent drug–DNA interactions

“…Every nucleus with its different characteristic spin gives rise to a signal or peak which represents a transition. 1 H is the most commonly used atomic nuclei for DNA binding study, but to study the effects of ligand binding on the phosphate groups of DNA, 31 P NMR is also used [86]. Two main features of NMR spectra studied in drug-DNA interaction are the change in line widths and chemical shifts on interaction [87].…”

Importance of fluorine in 2,3-dihydroquinazolinone and its interaction study with lysozyme

“…The concentration of the protein was maintained very low because of its increase in the viscosity. Both ligand and the protein solution were mixed together and allowed to attain equilibrium for few minutes [33].…”

Investigation on the interaction of letrozole with herring sperm DNA through spectroscopic and modeling methods

“…Thus, changes in the DNA FTIR spectra before and after adding drugs can reflect whether and where the interaction occurs. Several studies (26,49,50) have shown that changes in the wavenumber and intensity of the base and phosphate backbone can determine whether the interaction between drug and DNA can occur, as well as distinguish the binding modes. The bands at 1225 and 1097 cm À1 denoted PO 2 asymmetric and symmetric phosphate vibrations, respectively (48).…”

“…The bands at 1225 and 1097 cm À1 denoted PO 2 asymmetric and symmetric phosphate vibrations, respectively (48). Several studies (26,49,50) have shown that changes in the wavenumber and intensity of the base and phosphate backbone can determine whether the interaction between drug and DNA can occur, as well as distinguish the binding modes.…”