Proposed mechanism of antibacterial mode of action of <i>Caesalpinia bonducella</i> seed oil against food-borne pathogens

Abstract

&lt;p class="Abstract"&gt;The antibacterial mechanism of action of &lt;em&gt;Caesalpinia bonducella&lt;/em&gt; seed oil on membrane permeability of &lt;em&gt;Listeria monocytogenes&lt;/em&gt; NCIM 24563 (MIC: 2 mg/mL) and &lt;em&gt;Escherichia coli&lt;/em&gt; ATCC 25922 (MIC: 4 mg/mL) was determined by measuring the extracellular ATP concentration, release of 260-nm absorbing materials, leakage of potassium ions and measurement of relative electrical conductivity of the bacterial cells treated at MIC concentration. Its mode of action on membrane integrity was confirmed by release of extracellular ATP (1.42 and 1.33 pg/mL), loss of 260-nm absorbing materials (4.36 and 4.19 optical density), leakage of potassium ions (950 and 1000 mmol/L) and increase in relative electrical conductivity (12.6 and 10.5%) against food-borne pathogenic bacteria &lt;em&gt;L. monocytogenes&lt;/em&gt; and &lt;em&gt;E. coli&lt;/em&gt;, respectively. These findings propose that &lt;em&gt;C. bonducella&lt;/em&gt; oil compromised its mode of action on membrane integrity, suggesting its enormous food and pharmacological potential.&lt;/p&gt;&lt;p&gt; &lt;/p&gt;

Microbicidal Potentialities of Essential Oil from Pogostemon benghalensis (Burm.F.) Kuntze. and P. cablin (Blanco) Benth.

“…Therefore, abnormalities to the structural integrity of cell membrane can detrimentally affect cell metabolism and lead to cell death. Some Eos was recognized to have membrane active properties against several microorganisms, causing leakage of cell constituents, including ions [24,25,26,27]. Thus, the overall results of the present study showed a remarkable increase in the leakage of ions.…”

“…Further, the antibacterial effect may be also through derailment of proton motive force, regulating mitochondrial respiration, substrate oxidation, active transportation, and loss of pool biometabolites, as well as disruption of synthesis of macromolecules such as proteins, lipids and polysaccharides. In general, leakage of intracellular materials is induced by any antimicrobial agent results into the death of the cell [25]. The obtained activities may be attributed by the presence of several biologically active components present in the Eos of Pogostemon species.…”

Composition, antibacterial and anti-oxidant potentials of the essential oil of Hedychium matthewii

“…Recently, the antibacterial activity of essential oils of Buddleja asiatica, Caesalpinia bonducella, Ginkgo biloba and Ligustrum lucidum are reported (Bajpai et al, 2015;Bajpai et al, 2016;Khan et al, 2015;Shukla et al, 2016). Previous studies showed that multiplication of pathogenic Salmonella spp., Escherichia coli (O157:H7), Listeria monocytogenes, Helicobacter pylori and Mycoplasma pneumoniae were inhibited by essential oils (Burt, 2004).…”