Proposals for a Reappraisal of the Status of the Names of Plant-Pathogenic Pseudomonas Species

Dye, D. W.; Bradbury, J. F.; Dickey, R. S.; Goto, Masaaki; Hale, Chris; Hayward, Andrew; Kelman, A.; Lelliott, R. A.; Patel, Poorvi; Sands, David C.; Schroth, M. N.; Watson, D. R. W.; Young, J. M.

doi:10.1099/00207713-25-3-252

Cited by 20 publications

(12 citation statements)

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“…Recently, a recommendation has been made for the rejection of the epithet panici, which was not meant to be applied to fluorescent organisms (11); however, the strain included in our studies, as well as in the studies of other workers (3,7,28,30), is definitely a pathovar of P. syringae, although its origin is unclear.…”

Section: Resultscontrasting

confidence: 43%

Deoxyribonucleic Acid Similarities among Pseudomonas Species

Johnson

Palleroni

1989

International Journal of Systematic Bacteriology

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Deoxyribonucleic acid similarity experiments in which the S1 nuclease methodology was used were performed on strains of Pseudornonas species, including some that had not been previously subjected to similarity measurements. The results obtained with this technique confirmed and expanded previous observations on the low levels of deoxyribonucleic acid similarity among species of the genus as determined by membrane competition experiments. A general discussion of the results and nomenclatural status of some of the species is included.The genus Pseudornonas has been subdivided into five groups on the basis of ribosomal ribonucleic acid (rRNA)-deoxyribonucleic acid (DNA) hybridization experiments (23,24,27). This classification has received ample confirmation from work done in various laboratories in which a number of different experimental approaches have been used. In the years since it was first proposed, no significant changes have been required, with the exception of assignment of organisms to some of the groups of some Pseudomonas species that were not included in the original studies. Thus, in the recent rRNA-DNA hybridization study of Byng et al. (3), Pseudomonas panici, Pseudomonas synxantha, Pseudomonus angulata, and Pseiidomonas frugi were found to belong to group I; Pseudomonas pyrrocinia and Pseudomonas andropogorzis were found to belong to group 11; and Pseudomonas geniculata was found to belong to group V. In addition, Alcaligenes paradoxus was found to be related to organisms belonging to group 11, and Alcaligenes eutrophus was found to be related to group 111 organisms.The homogeneity within each Pseudomonas rRNA similarity cluster is very high, with most species having nearly 100% rRNA similarity (3, 27), although the levels of DNA similarity between species within each rRNA cluster are low (25). In contrast, we have not always found this clear clustering among groups of anaerobic bacteria (16)(17)(18). In order to confirm this rather interesting observation on the Pseudomonas groups and to extend DNA similarity measurements between additional species of the genus, the experiments described here were performed. In addition, some of the species were not included on the 1980 Approved Lists of Bacterial Names (33), and others appear to be synonyms. Nomenclatural proposals concerning some of these organisms are included below. MATERIALS AND METHODSBacterial strains. The organisms used in this study were the same as those used in a previous rRNA similarity study (3). All were obtained from the American Type Culture Collection, Rockville, Md.DNA preparation. The organisms were grown in a medium containing 1% tryptone (BBL Microbiology Systems, Cockeysville, Md.), 0.5% yeast extract (Difco Laboratories, Detroit, Mich.), and 0.5% glucose. Erlenmeyer flasks (volume, 2 liters) containing 500 ml of medium were inoculated with 5-ml portions of broth cultures and shaken overnight at 250 rpm on a rotary shaker at 30°C. The harvested cells were suspended in a 0.15 M NaCl-0.01 M ethylenediaminetetraacetic acid sal...

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Section: Resultscontrasting

confidence: 43%

Deoxyribonucleic Acid Similarities among Pseudomonas Species

Johnson

Palleroni

1989

International Journal of Systematic Bacteriology

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“…Other species of Pseudomonas reported by Dye et al ( , 1975 are identical to established species, or have been placed inappropriately in the genus.…”

Section: Genus I Acetobactermentioning

confidence: 99%

“…However, many pathogens which are legitimate species were not examined. Dye et al ( , 1975 published a list of legitimate species for which cultures are available, all of which are assigned to species in Pseudomonas or other genera, in the treatment below.…”

Section: Genus I Acetobactermentioning

confidence: 99%

A proposed nomenclature and classification for plant pathogenic bacteria

Young

Dye

Bradbury

et al. 1978

New Zealand Journal of Agricultural Research

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322

166

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“…Dye et al (13) suggested that all validly published names of plant pathogenic species of Pseudomonas for which there are isolates be retained until further studies have established their relationships accurately. This paper reports a taxonomic study of P. pomi and of strains identified as belonging to P. melophthora.…”

Section: Pseudomonas Melophthoramentioning

confidence: 99%

Pseudomonas pomi Cole 1959 Is a Later Subjective Synonym of Acetobacter pasteurianus (Hansen 1879) Beijerinck 1898, and Pseudomonas melophthora Allen and Riker 1932 is a Nomen Dubium

Dhanvantari¹,

Dye²,

Young³

1978

International Journal of Systematic Bacteriology

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An examination of all known strains labeled Pseudomonas melophthora Allen and Riker 1-n-desi gnat ed type strain of P. pomi Cole 1959 (strain PDDCC 3878 [= NCPPB 4631) showed that they were similar to one another but atypical of members of the genus Pseudomonas Migula 1894. Because these organisms were found to be either motile (peritrichous cells) or nonmotile, able to grow at low pH values, and to oxidize ethanol and lactate to carbonate, it is suggested that they belong to Acetobacter Biejerinck 1898. P. pomi is regarded here as being a subjective junior synonym of A. pasteurianus (Hansen 1897) Beije$nck 1898, and P. melophthora is regarded here as being a nomen dubium.Isolates of P. pomi and similar organisms capable of inducing necrosis in apple fruit should be classified in A. pasteurianus until the relationships of pathogenic strains within the species are better understood.Pseudomonas melophthora Allen and Riker (1) was first reported in the United States as causing a firm brown discoloration of apple (Malus pumila Mill.) fruit tissue after invasion by apple maggots. It and another bacterial pathogen, Pseudomonas pomi Cole (7), were later reported in England. The latter organism produced a similar effect but, unlike P. melophthora, it was encapsulated and nonmotile.Cole deposited isolates of both of these organisms with the National Collection of Plant Pathogenic Bacteria (NCPPB), Harpenden, England. One isolate of P. pomi and two identified as P. melophthora, all from the NCPPB, were the only strains of these species available for study.In their revision of Pseudomonas Migula (25), Doudoroff and Pderoni (11) included P. melophthora in an addendum containing a list of incompletely described species and placed P. pomi in an addendum containing a list of species known to possess characters not in accord with the generic definition; as such, these organisms were regarded as species incertae sedis. Dye et al. (13) suggested that all validly published names of plant pathogenic species of Pseudomonas for which there are isolates be retained until further studies have established their relationships accurately. This paper reports a taxonomic study of P. pomi and of strains identified as belonging to P. melophthora. Media. For routine testing, cells were subcultured on potato-dextrose agar (Difco Laboratories) and incubated for 4 to 7 days at 25°C. Unless otherwise indicated, faintly turbid suspensions (ca. 10' colonyforming units per ml) were prepared in sterile distilled water for the inoculation of broth and agar media. MATERiIALS AND METHODS BacterialGrowth of cultures was compared on: nutrient agar; potato-dextrose agar; yeast-salts agar (12) Gram reaction, motility, and flagellation. The Hucker modification of the Gram stain was used, in which Lugol iodine replaced Burke iodine (27). The periods of staining, mordanting, counter-staining, and rinsing were carehlly timed (3).Motility was determined with 48-h-old shake cultures (yeast-salts-2% glucose broth) observed with a phase-contrast microscope. F...

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Proposals for a Reappraisal of the Status of the Names of Plant-Pathogenic Pseudomonas Species

Cited by 20 publications

References 0 publications

Deoxyribonucleic Acid Similarities among Pseudomonas Species

Deoxyribonucleic Acid Similarities among Pseudomonas Species

A proposed nomenclature and classification for plant pathogenic bacteria

Pseudomonas pomi Cole 1959 Is a Later Subjective Synonym of Acetobacter pasteurianus (Hansen 1879) Beijerinck 1898, and Pseudomonas melophthora Allen and Riker 1932 is a Nomen Dubium

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