Trans-femoral amputees must regain moving pattern by refined rehabilitation program using loads applied on a prosthetic limb. On the other hand, understanding those loads is indispensable for biomechanical consideration of trans-femoral amputees. However, conventional prosthetic gait training systems cannot measure long continuous walking motions. In this paper, loads applied on trans-femoral prosthesis are measured by the prosthetic gait training system for the unrestrained gait measurement. As a result of the experiments, the patterns of moments about the medio-lateral axis are remarkably different among the six activities. Finally, the effectiveness of the developed prosthetic gait training system to analyze biomechanics in trans-femoral prosthesis is validated.