Proposal of Four New Aureobasidium Species for Exopolysaccharide Production

Abstract

In this study, 99 strains of Aureobasidium species were isolated from various samples collected from different locations in China, among which 14 isolates showed different morphological characteristics to other strains identified as known Aureobasidium species. Based on morphological characteristics, those 14 strains were classified into four groups, represented by stains of KCL139, MDSC−10, XZY411−4, and MQL9−100, respectively. Molecular analysis of the internal transcriptional spacer (ITS) and part of the large ribosome subunit (D1/D2 domains) indicated that those four groups represent four new species in the Aureobasidium. Therefore, the names Aureobasidium insectorum sp. nov., A. planticola sp. nov., A. motuoense sp. nov., and A. intercalariosporum sp. nov. are proposed for KCL139, MDSC−10, XZY411−4, and MQL9−100, respectively. We also found that there were differences in the yield of exopolysaccharides (EPS) among and within species, indicating strain-related exopolysaccharide-producing diversity.

Use of Aureobasidium in a sustainable economy

“…Currently, 32 DNA-identified Aureobasidium species are known (Table 1 ). These species include the best known Aureobasidium species, Aureobasidium pullulans, as well as the most recently identified species Aureobasidium insectorum , Aureobasidium planticola , Aureobasidium motuoense , and Aureobasidium intercalariosporum (Arnaud 1918 ; Arzanlou and Khodaei 2012 ; Ashish and Pratibha 2018 ; Barr 2001 ; Bills et al 2012 ; Ciferri et al 1957 ; Cooke 1962 ; Crous et al 2021 , 2011 ; de Hoog and Hermanides-Nijhof 1977a , 1977b ; Gostinčar et al 2014 ; Inamdar et al 2019 ; Jia et al 2019 ; Jiang et al 2021 , 2019 ; Lee et al 2021 ; Nasr et al 2018 ; Onetto et al 2020 ; Peterson et al 2013 ; Ramaley 1992 ; Wang et al 2022a ; Wu et al 2023 ). An additional 15 species have been identified based on morphology (Table 2 ) (Cooke 1962 ; Crisan and Hodisan 1964 ; Della Torre 1963 ; de Hoog and Hermanides-Nijhof 1977a ; Pande and Ghate 1985 ; Richardson and Pitkäranta 2011 ).…”

“…Wang, F. Wu, and M.M. Wang 2023 Spittle insects China OP856707, OP857208 Wu et al ( 2023 ) Aureobasidium intercalariosporum Q.M. Wang, F. Wu, and M.M.…”

“…Wang, F. Wu, and M.M. Wang 2023 Leaf China OP856703, OP857205 Wu et al ( 2023 ) Aureobasidium iranianum Arzanlou and Khodaei 2012 Bamboo Iran MB800705, CCTU 268 Arzanlou and Khodaei ( 2012 ) Aureobasidium khasianum J. Pratibha and Prabhug 2018 Decaying leaves of Wightia speciosissima India MB828278AVP 109 Ashish and Pratibha ( 2018 ) Aureobasidium leucospermi Crous 2011 Leucospermum conocarpodendron leaves Stellenbosch, South Africa MB560556, CBS 130593 Crous et al ( 2011 ) Kabatiella lini Aureobasidium lini (Laff.) Hermanides-Nijhof 1977 Linum usitatissimum UK MB283371, CBS 125.21 de Hoog and Hermanides-Nijhof ( 1977a ) Aureobasidium mangrovei S. Nasr 2018 Healthy Avicennia marina plant Qeshm Island, Iran MB823444, IBRC M 30265 Nasr et al ( 2018 ) Aureobasidium melanogenum (Hermanides-Nijhof) Zalar, Gostincar, and Gunde-Cimerman 2014 N/A N/A MB807698, CBS 105.22 …”

“…Wang, F. Wu, and M.M. Wang 2023 Leaf China OP856710, OP857211 Wu et al ( 2023 ) Aureobasidium mustum C. Onetto, S. Schmidt, M. Roach, and A. Borneman 2020 Fresh grape juice South Australia MB836845 Onetto et al ( 2020 ) Aureobasidium namibiae (Zalar, de Hoog and Gunde-Cimerman) Zalar, Gostincar, and Gunde-Cimerman 2014 Dolomitic marble Namib Desert, Namibia MB807701, CBS 147.97 Gostinčar et al ( 2014 ) Aureobasidium pini C.M. Tian and N. Jiang 2019 Pine needle China MB828664, CFCC 52778 Jiang et al ( 2019 ) Aureobasidium planticola Q.M.…”

“…Wang, F. Wu, and M.M. Wang 2023 Leaf China OP856711, OP857212 Wu et al ( 2023 ) Aureobasidium proteae (Joanne E. Taylor and Crous) Joanne E. Taylor and Crous 2011 Leaves of Protea cv. ‘ Sylvia ’ South Africa MB560557, CBS 114273 Crous et al ( 2011 ) Aureobasidium pullulans (De Bary) G. Arnaud ex Cif., Ribaldi, and Corte 1957 Vitis vinifera, fruit Beaujolais, Beaujeu, France MB508998, CBS 584.75 Ciferri et al ( 1957 ) and Gostinčar et al ( 2014 ) Aureobasidium subglaciale (Zalar, de Hoog and Gunde-Cimerman) Zalar, Gostincar, and Gunde-Cimerman 2014 Subglacial ice from sea water Kongsvegen, Svalbard, Norway MB807700, CBS 123387 Gostinčar et al ( 2014 ) Aureobasidium thailandense S. W. Peterson, Manitchotpisit, and Leathers 2013 Wood surface Prachuapkhirikhan, Thailand MB801148, NRRL 58543 Peterson et al ( 2013 ) Aureobasidium tremulum Inamdar, Roh.…”