Proposal of Ensifer psoraleae sp. nov., Ensifer sesbaniae sp. nov., Ensifer morelense comb. nov. and Ensifer americanum comb. nov

Wang, Yuan Chun; Wang, Fang; Hou, Bao Chao; Wang, En Tao; Chen, Wen Feng; Sui, Xin; Li, Yán; Zhang, Ya Bo

doi:10.1016/j.syapm.2013.05.001

Cited by 57 publications

(20 citation statements)

References 29 publications

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“…Ensifer kummerowiae, Ensifer medicae, Ensifer meliloti, Ensifer mexicanus, Ensifer morelense, Ensifer numidicus, Ensifer psoraleae, Ensifer saheli, Ensifer sesbaniae, Ensifer sojae and Ensifer terangae (Casida Jr, 1982;Li et al, 2011;Lloret et al, 2007;Martens et al, 2008;Merabet et al, 2010;Wang et al, 2013;Yan et al, 2016;Young, 2003). In order to clarify the accurate taxonomic position of this novel Ensifer group, we performed the present study to compare it with the defined Ensifer species.…”

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confidence: 99%

Ensifer alkalisoli sp. nov. isolated from root nodules of Sesbania cannabina grown in saline–alkaline soils

Yan

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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A group of Sesbania cannabina rhizobia belonging to four recA genotypes of a novel group was further characterized in comparison with the related Ensifer species. They showed 98.2 to 99.9 % similarities among themselves and 92.9 to 93.3 % similarities with the most related strain Ensifer sojae CCBAU 05684 T in multilocus sequence analysis of recA, atpD and glnII. The genome average nucleotide identity values between representative strain YIC4027 T and the type strains of its closely related species were 81.6 to 88.9 %. Identical symbiotic gene (nodA, nodC and nifH) sequences highly similar with those in other Sesbania-nodulating strains (Rhizobium sp. SIN-1, Neorhizobium huautlense S02 T , Ensifer saheli ORS609 T and Rhizobium sp. IRBG74) were detected. The representative strain YIC4027 T could form effective nodules on its original host Sesbania cannabina, but not on Sophora flavescens, Trifolium repens, Glycine max, Glycyrrhiza uralensis, Phaseolus vulgaris or Medicago sativa. The use of lactulose as sole carbon source, possession of C 13 : 0 2-OH, C 13 : 1 at 12-13, C 15 : 1 iso !9c, C 17 : 0 anteiso and C 18 : 0 iso and absence of C 14 : 0 anteiso, C 15 : 0 anteiso and C 18 : 0 3-OH in fatty acids distinguished the strain YIC4027 T from the type strains of its closely related species. Based on all the analyses mentioned above, we propose a novel species Ensifer alkalisoli sp. nov. and designate YIC4027 T (=HAMBI 3655 T =LMG 29286 T ) as the type strain. The genome size of YIC4027 T is 5.97 Mbp, comprising 5588 predicted genes, and the DNA G+C content is 62.2 mol%.

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confidence: 99%

Ensifer alkalisoli sp. nov. isolated from root nodules of Sesbania cannabina grown in saline–alkaline soils

Yan

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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“…To end the debate, the Judicial Commission of the International Committee on Systematics of Prokaryotes made a final decision that Ensifer takes priority over Sinorhizobium and should be used at least in official journals, such as the International Journal of Systematic and Evolutionary Microbiology (Young, 2010). Since then, the names of eight novel species and new combinations have been validly published within the genus Ensifer, including Ensifer garamanticus (Merabet et al, 2010), Ensifer numidicus (Merabet et al, 2010), Ensifer mexicanus (Lloret et al, 2007), Ensifer sojae (Li et al, 2011a), Ensifer americanus (Wang et al, 2013), Ensifer psoraleae (Wang et al, 2013), Ensifer sesbaniae (Wang et al, 2013) and Ensifer morelensis (Wang et al, 2013).…”

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confidence: 99%

Ensifer glycinis sp. nov., a rhizobial species associated with species of the genus Glycine

Yan

Sui

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Rhizobial strains from root nodules of Astragalus mongholicus and soybean (Glycine max) were characterized phylogenetically as members of the genus Ensifer (formerly named Sinorhizobium), based on 16S rRNA gene sequence comparisons. Results based upon concatenated sequence analysis of three housekeeping genes (recA, atpD and glnII, 93.8 % similarities to known species) and average nucleotide identity (ANI) values of whole genome sequence comparisons (ranging from 89.6 % to 83.4 % to Ensifer fredii and Ensifer saheli, respectively) indicated the distinct positions of these novel strains within the genus Ensifer. Phylogeny of symbiotic genes (nodC and nifH) of three novel strains clustered them with rhizobial species Ensifer fredii and Ensifer sojae, both isolated from nodules of Glycine max. Cross-nodulation tests showed that the representative strain CCBAU 23380 T could form root nodules with nitrogen fixation capability on Glycine soja, Albizia julibrissin, Vigna unguiculata and Cajanus cajan, but failed to nodulate Astragalus mongholicus, its original host legume. Strain CCBAU 23380 T formed inefficient nodules on G. max, and it did not contain 18 : 0, 18 : 1!7c 11-methyl or summed feature 1 fatty acids, which differed from other related strains. Failure to utilize malonic acid as a carbon source distinguished strain CCBAU 23380 T from the type strains of related species. The genome size of CCBAU 23380 T was 6.0 Mbp, comprising 5624 predicted genes with DNA G+C content of 62.4 mol%. Based on the results above, a novel species, Ensifer glycinis sp. nov., is proposed, with CCBAU 23380 T (=LMG 29231 T =HAMBI 3645 T ) as the type strain.

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“…However, a few exceptions were noted. E. sesbaniae , a known symbiont (Wang et al 2013) whose ability to effectively nodulate Phaseolus vulgaris was confirmed (Supplementary Figure S2), was found within the non-symbiotic clade. Similarly, at least one of the six symbiotic proteins were not detected in eight strains of the symbiotic group, although we cannot rule out that these are false negatives in our annotation pipeline.…”

Section: Resultsmentioning

confidence: 84%

“…Draft genomes of E. morelense Lc04, E. morelense Lc18, E. sesbaniae CCBAU 65729, and E. psoraleae CCBAU 65732 (Wang et al 1999, 2013) were generated to increase the species diversity available for our analyses. Summary statistics of the assemblies are provided in Supplementary Table S3.…”

Section: Resultsmentioning

confidence: 99%

Symbiotic and non-symbiotic members of the genusEnsifer(syn.Sinorhizobium) are separated into two clades based on comparative genomics and high-throughput phenotyping

Fagorzi

Ilie

Decorosi

et al. 2020

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Proposal of Ensifer psoraleae sp. nov., Ensifer sesbaniae sp. nov., Ensifer morelense comb. nov. and Ensifer americanum comb. nov

Cited by 57 publications

References 29 publications

Ensifer alkalisoli sp. nov. isolated from root nodules of Sesbania cannabina grown in saline–alkaline soils

Ensifer alkalisoli sp. nov. isolated from root nodules of Sesbania cannabina grown in saline–alkaline soils

Ensifer glycinis sp. nov., a rhizobial species associated with species of the genus Glycine

Symbiotic and non-symbiotic members of the genusEnsifer(syn.Sinorhizobium) are separated into two clades based on comparative genomics and high-throughput phenotyping

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