Proposal of biostimulation for hexachlorocyclohexane (HCH)-decontamination and characterization of culturable bacterial community from high-dose point HCH-contaminated soils

Dadhwal, Mandeep; Singh, Amit Kumar; Prakash, Om; Gupta, Sanjay; Kumari, Kavita; Sharma, Poonam; Jit, Simran; Verma, Mansi; Holliger, Christof; Lal, Rup

doi:10.1111/j.1365-2672.2008.03982.x

Cited by 60 publications

(42 citation statements)

References 44 publications

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“…There is also evidence from DNA hybridization studies that some other strains may also have two copies of the linA gene (31). The LinA1 and LinA2 enzymes from B90A are known to differ by about 10% in their amino acid sequences.…”

Section: Variants Of Linamentioning

confidence: 99%

“…Some of the spread of lin genes across species, and in particular within species, is instead attributed to their association with IS6100 insertion sequences. Most of the lin genes found to date are in fact associated with IS6100, both within (20,27,31,40,62,103,109) and outside (161) (Table 3) the Sphingomonadaceae. IS6100 sequences have also been associated with the spread of genes for degrading antibiotics (170,172) and other pesticides (59,81).…”

Section: Evolutionary Issues and Scope For Improvementmentioning

confidence: 99%

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Biochemistry of Microbial Degradation of Hexachlorocyclohexane and Prospects for Bioremediation

Lal

Pandey

Sharma

et al. 2010

Microbiol Mol Biol Rev

348

338

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SUMMARY Lindane, the γ-isomer of hexachlorocyclohexane (HCH), is a potent insecticide. Purified lindane or unpurified mixtures of this and α-, β-, and δ-isomers of HCH were widely used as commercial insecticides in the last half of the 20th century. Large dumps of unused HCH isomers now constitute a major hazard because of their long residence times in soil and high nontarget toxicities. The major pathway for the aerobic degradation of HCH isomers in soil is the Lin pathway, and variants of this pathway will degrade all four of the HCH isomers although only slowly. Sequence differences in the primary LinA and LinB enzymes in the pathway play a key role in determining their ability to degrade the different isomers. LinA is a dehydrochlorinase, but little is known of its biochemistry. LinB is a hydrolytic dechlorinase that has been heterologously expressed and crystallized, and there is some understanding of the sequence-structure-function relationships underlying its substrate specificity and kinetics, although there are also some significant anomalies. The kinetics of some LinB variants are reported to be slow even for their preferred isomers. It is important to develop a better understanding of the biochemistries of the LinA and LinB variants and to use that knowledge to build better variants, because field trials of some bioremediation strategies based on the Lin pathway have yielded promising results but would not yet achieve economic levels of remediation.

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Section: Variants Of Linamentioning

confidence: 99%

Section: Evolutionary Issues and Scope For Improvementmentioning

confidence: 99%

Biochemistry of Microbial Degradation of Hexachlorocyclohexane and Prospects for Bioremediation

Lal

Pandey

Sharma

et al. 2010

Microbiol Mol Biol Rev

348

338

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“…These sites serve as reservoirs from where HCH residues are spreading to far off regions due to leaching and aerial transport. Such HCH dumpsites are present all over the world and have been reported from Rio de Janeiro (Brazil) [23] , Pontevedra (Spain) [24,25] , Bilbao (Spain) [26], Chemnitz (Germany) [27], North Carolina (US) [http://www.earthfax.com/WhiteRot/PCP.htm] and Bitterfi eld (Germany) [28], Lucknow (India) [29]. These reports are just a small proportion of dumpsites brought to public notice.…”

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confidence: 99%

Pseudomonas sp. to Sphingobium indicum: a journey of microbial degradation and bioremediation of Hexachlorocyclohexane

et al. 2008

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The unusual process of production of hexachlorocyclohexane (HCH) and extensive use of technical HCH and lindane has created a very serious problem of HCH contamination. While the use of technical HCH and lindane has been banned all over the world, India still continues producing lindane. Bacteria, especially Sphingomonads have been isolated that can degrade HCH isomers. Among all the bacterial strains isolated so far, Sphingobium indicum B90A that was isolated form HCH treated rhizosphere soil appears to have a better potential for HCH degradation. This conclusion is based on studies on the organization of lin genes and degradation ability of B90A. This strain perhaps can be used for HCH decontamination through bioaugmentation.

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“…Methods described for obtaining the T-RF patterns are more or less similar with slight modifications [7,8,16]. It constitutes extraction of total community DNA, PCR amplification of molecular marker with labeled primer(s), purification of amplified products, restriction digestion of the purified amplicon with one or more restriction endonucleases (notably the 4-base cutters) and finally separation of T-RFs by capillary electrophoresis using an automated DNA sequencer equipped with data collection and analysis software [8].…”

Section: Introductionmentioning

confidence: 99%

“…It is an inexpensive, robust, reproducible, and rapid method for the study of microbial community structure. The potential of T-RFLP method for the study of community dynamics and structure is extensively studied for diverse habitats [5][6][7][8][9][10][11][12][13][14]. In addition to 16S rRNA gene, T-RFLP analysis also included genes representing a variety of functional groups [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Technicalities and Glitches of Terminal Restriction Fragment Length Polymorphism (T-RFLP)

et al. 2014

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Terminal restriction fragment length polymorphism (T-RFLP) is a rapid, robust, inexpensive and simple tool for microbial community profiling. Methods used for DNA extraction, PCR amplification and digestion of amplified products have a considerable impact on the results of T-RFLP. Pitfalls of the method skew the similarity analysis and compromise its high throughput ability. Despite a high throughput method of data generation, data analysis is still in its infancy and needs more attention. Current article highlights the limitations of the methods used for data generation and analysis. It also provides an overview of the recent methodological developments in T-RFLP which will assist the readers in obtaining real and authentic profiles of the microbial communities under consideration while eluding the inherent biases and technical difficulties.

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Proposal of biostimulation for hexachlorocyclohexane (HCH)-decontamination and characterization of culturable bacterial community from high-dose point HCH-contaminated soils

Abstract: The study demonstrates that biostimulation of indigenous HCH-degrading microbial population can be used for decontamination of chronically HCH-contaminated sites.

Cited by 60 publications

References 44 publications

Biochemistry of Microbial Degradation of Hexachlorocyclohexane and Prospects for Bioremediation

Biochemistry of Microbial Degradation of Hexachlorocyclohexane and Prospects for Bioremediation

Pseudomonas sp. to Sphingobium indicum: a journey of microbial degradation and bioremediation of Hexachlorocyclohexane

Technicalities and Glitches of Terminal Restriction Fragment Length Polymorphism (T-RFLP)

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