Proposal of a subtype of serovar 4, K4b:O3, of Actinobacillus pleuropneumoniae based on serological and genotypic analysis

To, Ho; Kon, Michiha; Koike, Fumihiko; Shibuya, Kazumoto; Nagai, Shinya; Gottschalk, Marcelo; Frey, Joachim; Sasakawa, Chihiro

doi:10.1016/j.vetmic.2021.109279

Cited by 5 publications

(11 citation statements)

References 23 publications

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“…Clinically, PCP is mainly characterized by acute fibrinous hemorrhagic pleuropneumonia and chronic fibrinous necrotizing pleuropneumonia (Sassu et al 2018). So far, APP is divided into 2 biotypes and 19 serovars (Bosse et al 2018;Sassu et al 2018;To et al 2021;Stringer et al 2021). Some factors associated with virulence such as toxins, lipopolysaccharide, adhesion molecules, and outer membrane proteins contribute to the pathogenicity (Chiers et al 2010).…”

Section: Introductionmentioning

confidence: 99%

The QseB/QseC two-component system contributes to virulence of Actinobacillus pleuropneumoniae by downregulating apf gene cluster transcription

et al. 2022

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Actinobacillus pleuropneumoniae (APP) is the major pathogen of porcine contagious pleuropneumoniae (PCP). The QseB/QseC two-component system (TCS) consists of the regulator QseB and the kinase QseC, which relates to quorum sensing (QS) and virulence in some bacteria. Here, we investigated the role of QseB/QseC in apf gene cluster (apfABCD) expression of APP. Our results have showed that QseB/QseC TCS can potentially regulate the expression of apf gene cluster. The ΔqseBC, ΔapfA, ΔapfB, ΔapfC and ΔapfD strains are more sensitive to acidic and osmotic stressful conditions, and exhibite lower biofilm formation ability than wild-type (WT) strain, whereas the complemented strains show similar phenotype to the WT strain. In additon, the mutants have defective anti-phagocytosis, adhesion and invasion when they come into contact with the host cells. In experimental animal models of infection, mice infected with ΔqseBC, ΔapfA, ΔapfB, ΔapfC and ΔapfD strains showed lower mortality and bacterial loads in the lung and the blood than those infected with WT strain. In conclusion, our results suggest that QseB/QseC TCS contributes to stress resistance, biofilm formation, phagocytosis, adhesion, invasion and virulence by downregulating expression of apf gene cluster in A. pleuropneumoniae.

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Section: Introductionmentioning

confidence: 99%

The QseB/QseC two-component system contributes to virulence of Actinobacillus pleuropneumoniae by downregulating apf gene cluster transcription

et al. 2022

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“…In order to elucidate the reason for the discrepancy between molecular and serological typing for the isolate OT761, the gene clusters involved in the biosynthesis of CPS and/or O-PS were PCR-amplified from the isolate OT761, and the four isolates of serovar 2 (A-D). The primer pairs used for amplification of the cps [1,8,28] and o-ps [26] genes are listed in Table 1. The PCR was performed as described previously [27].…”

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confidence: 99%

“…The PCR was performed as described previously [27]. Purification of PCR products and determination of the nucleotide sequences were performed as described previously [26,27].…”

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confidence: 99%

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Characterization of an atypical <i>Actinobacillus pleuropneumoniae</i> serovar 2 isolate with a rough-type lipopolysaccharide

Akaike

Kon

et al. 2023

The Journal of Veterinary Medical Science

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We describe phenotypic and genetic characterization of an atypical Japanese Actinobacillus pleuropneumoniae isolate OT761. Nucleotide sequence analysis revealed that gene clusters involved in capsular polysaccharide and O-polysaccharide (O-PS) biosynthesis of the isolate were nearly identical to those of serovar 2 reference strain. The main difference found between the O-PS loci is the shortening of 31 amino acids from the C terminus of WcaJ in the atypical isolate due to a 93 bp deletion at the 3' end of wcaJ gene. Immunoblot analysis revealed that this isolate could not produce O-PS. Taken together, our results showed that the C-terminal domain of the A. pleuropneumoniae WcaJ plays a critical role in enzyme function of WcaJ involved in the biosynthesis of O-PS.

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“…2,8,16 For example, serovars 1, 9, and 11 would be designated as serovars K1:O1, K9:O9, and K11:O1. 2 Rare isolates (serovar or subtype) have also been reported (e.g., strains of K2:O7, 16 K19:O4, K19:O3, 26 K1:O7, 8 K13:O10, 18 K4b:O3, 28 and K12:O3 10 ). We report here the characterization of 3 nonencapsulated A. pleuropneumoniae isolates of K12:O3.…”

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Characterization of nonencapsulated Actinobacillus pleuropneumoniae serovar K12:O3 isolates

To,

Tsutsumi,

Ito

et al. 2023

J VET Diagn Invest

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Three Actinobacillus pleuropneumoniae isolates from clinical cases of porcine pleuropneumonia were positive by capsular serovar 12–specific PCR assay, but not reactive to antiserum prepared against serovar 12 using the rapid slide agglutination (RSA) test. The isolates were positive for apxIICA, apxIIICA, apxIBD, apxIIIBD, and apxIVA in the PCR toxin gene assay, which is the profile seen in serovars 2, 4, 6, 8, and 15, and reacted with antisera against serovars 3, 6, 8, 15, and 17. Nucleotide sequence analysis revealed that genes involved in the biosynthesis of capsular polysaccharide of the 3 isolates were identical or nearly identical to those of serovar 12. However, genes involved in the biosynthesis of O-polysaccharide of the 3 isolates were highly similar to those of reference strains of serovars 3, 6, 8, 15, 17, and 19. In agreement with results from the RSA test, transmission electron microscopic analysis confirmed the absence of detectable capsular material in the 3 isolates. The existence of nonencapsulated A. pleuropneumoniae serovar K12:O3 would hamper precise serodetection.

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Proposal of a subtype of serovar 4, K4b:O3, of Actinobacillus pleuropneumoniae based on serological and genotypic analysis

Cited by 5 publications

References 23 publications

The QseB/QseC two-component system contributes to virulence of Actinobacillus pleuropneumoniae by downregulating apf gene cluster transcription

The QseB/QseC two-component system contributes to virulence of Actinobacillus pleuropneumoniae by downregulating apf gene cluster transcription

Characterization of an atypical <i>Actinobacillus pleuropneumoniae</i> serovar 2 isolate with a rough-type lipopolysaccharide

Characterization of nonencapsulated Actinobacillus pleuropneumoniae serovar K12:O3 isolates

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