Proposal and application of an ecotoxicity biotest based on escherichia coli

Espigares, M.; Roman, Ioana; Alonso, J. M. Gonzalez; Luis, B. de; Yeste, F.; Gálvez, Ramón

doi:10.1002/jat.2550100611

Cited by 7 publications

(7 citation statements)

References 15 publications

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“…39,40 The toxic effect is assessed by testing growth inhibition of the bacterial strain Escherichia coli K12 W3110 thy − (CECT 416, Spanish Type Culture Collection). The assay was carried out in tubes containing 10 ml of Vogel-Bonner culture medium supplemented with 50 mg 1 −1 of thymine and 20 g 1 −1 of glucose.…”

Section: Igec Bioassaymentioning

confidence: 99%

“…Some data regarding the predictive power of this biotest have been published previously. 39,40 The objective of the present study was to assess the toxicity of a group of newly synthesized DTCs using the IGEC bioassay, and to evaluate statistical models based on quantitative structure-activity relationships for estimating the possible relationship between some chemical characteristics of these compounds and their degree of toxicity.…”

Section: Introductionmentioning

confidence: 99%

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In vitro toxicity of several dithiocarbamates and structure–activity relationships

Segovia

Crovetto

Lardelli

et al. 2002

J of Applied Toxicology

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Dithiocarbamates (DTCs) are chemicals featuring a great chelating capacity. The toxicological study of DTCs is very important in view of their relatively simple synthesis and wide array of sanitary and industrial applications. In this study, the toxicity of some of the more recently synthesized DTCs is determined using an extremely simple bioassay, described in previous studies, based on the inhibition of growth of Escherichia coli (IGEC). The lowest-observed-effect concentration (LOEC), the median effective concentration (EC(50)) and no-observed-effect concentration (NOEC) of the following sodium dithiocarbamates was determined: N-benzyl-N-methyldithiocarbamate x 2H(2)O, N-benzyl-N-isopropyldithiocarbamate x 3H(2)O, N-benzyl-N-ethyldithiocarbamate x 2H(2)O, N-butyl-N-methyldithiocarbamate x 2H(2)O, N,N-dibenzyldithiocarbamate x 2H(2)O and N-benzyl-2-phenethyldithiocarbamate x 4H(2)O. Our results showed N,N-dibenzyl-DTC to be the least toxic of the tested substances, with an EC(50) value of 1,269.9 micro g ml(-1), whereas N-butyl-N-methyl-DTC and N-benzyl-N-methyl-DTC, with respective EC(50) values of 14.9 micro g ml(-1) and 23.5 micro g ml(-1), were the most toxic. Regression analysis showed, through exponential models, that the degree of toxicity of this group of substances correlated with the molecular weight of the compound, the molecular weight of the smallest chemical radical linked to the dithiocarbamate group and the number of benzene rings present in the molecule. The consideration of these models allows us to establish that in general terms the toxicity of DTCs decreases exponentially with a greater molecular weight and the number of benzene rings.

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Section: Igec Bioassaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vitro toxicity of several dithiocarbamates and structure–activity relationships

Segovia

Crovetto

Lardelli

et al. 2002

J of Applied Toxicology

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“…As can be seen in Table I, subinhibiting concentrations of nickel and methyl-parathion were used with a view to the possible application of the measurement of the E. coli peroxidation levels as a marker for ecotoxicity biotests. The sensitivity of this method would ideally be equal to or greater than that of simpler techniques, such as those based on the inhibition of growth (Espigares et al, 1990).…”

Section: Discussionmentioning

confidence: 99%

“…The bacterial strain Escherichia coli K-12 W3110 thy-F-was incubated in Vogel-Bonner medium supplemented with thymine (50 mg/L), glucose (20 g/L), and peptone (0.5 g/L) (Davies et al, 1980;Maron and Ames, 1983). Toxicants were added in previously determined subinhibiting concentrations of 40 pg/mL nickel and 62.5 pg/mL methyl-parathion (Espigares et al, 1990;Odberg-Ferragut et al, 1991).…”

Section: Methodsmentioning

confidence: 99%

“…In recent years, Escherichia coli has been used as a biological model for the development of relatively quick toxicity tests based on the inhibition of growth and on certain enzymatic alterations, such as the overproduction of superoxide dismutase as an oxidative stress response (Mamber et al, 1986;Cenci et al, 1987;Espigares and Mariscal, 1989;Espigares et al, 1990;Siwecki and Brown, 1990;Hassan and Fridovich, 1991;Imlay and Fridovich, 1991;Kwan, 1993). However, little is yet known about thiobarbituric acid reactivity in E. coli (Catallo et al, 1992), or how it might respond to the production of lipofuscin-like substances induced by different toxic substances (Sharonov and Kondrat'eva,199 1).…”

Section: Ntrodu Cti 0 Nmentioning

confidence: 99%

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Peroxidation levels in Eschericia coli as a potential toxicity marker: Effect of nickel and methyl‐parathion

Espigares¹,

Lucadamo²,

Coca³

et al. 1994

Environ. Toxicol. Water Qual.

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The effects of nickel and methyl‐parathion were studied in subinhibiting concentrations (40 and 62.5 μg/mL, respectively) on the levels of cellular peroxidation in Escherichia coli. These effects were evaluated using the thiobarbituric acid (TBA) test and the determination of lipofuscin‐like substances. Both toxicants caused an increase in cellular peroxidation levels, although at the concentrations used, nickel was shown to be a better inducer than methyl‐parathion. The TBA test gave more significant differences than the determination of lipofuscin‐like substances when the control cultures were compared to those containing the toxicant. This finding reveals that the TBA test could be used as a toxicity marker in E. coli for the development of ecotoxicity biotests. © 1994 by John Wiley & Sons, Inc..

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In vitro evaluation of the toxicity of several dithiocarbamates using anEscherichia coli growth inhibition bioassay and the acetylcholinesterase inhibition test

Espigares¹,

Crovetto²,

Gálvez³

1998

Environ. Toxicol. Water Qual.

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The industrial and sanitary use of dithiocarbamates DTCs is on the rise, and the synthesis of new derivatives has increased the field of their application. Both positive and negative pharmacological and toxicological effects have been documented for DTCs. The development and application of new 50 exhibited a minimal inhibitory effect on AChE, the results obtained indicate a generalized absence of AChE inhibition for the DTCs.

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Proposal and application of an ecotoxicity biotest based on escherichia coli

Cited by 7 publications

References 15 publications

In vitro toxicity of several dithiocarbamates and structure–activity relationships

In vitro toxicity of several dithiocarbamates and structure–activity relationships

Peroxidation levels in Eschericia coli as a potential toxicity marker: Effect of nickel and methyl‐parathion

In vitro evaluation of the toxicity of several dithiocarbamates using anEscherichia coli growth inhibition bioassay and the acetylcholinesterase inhibition test

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