Proportionalit�tsfehler bei der Feulgen-Hydrolyse

MACROMOLECULAR SYNTHESES RELATED TO THE REPRODUCTIVE CYST OF TETRAHYMENA PATULA

“…The induced red fluorescence was then collected by the objective lens, passed through the dichroic mirror and a K580 barrier filter (both of which transmit red light), the area of the nucleus optically isolated by the MPV diaphragm, and the image of the nucleus projected onto the photomultiplier S-20 type 9558AQ with a quartz window . Only a narrow band of the red spectrum was used for the measurement (Bohm and Sprenger, 1968) and was selected by a 60 mm interference wedge filter set at 660 nm. Thus, fluorescence intensities at 660 nm were recorded as photomultiplier voltage output and were proportional to the DNA content .…”

Morphoquantitative Analysis of the Intraepithelial Neoplasic Uterine Cells by Computer Image System

“…In fact, they made us disqualify several subjects' material (61.1%) for having a totally irregular distribution of chromogen results in diverse nucleus cells and a major intensity in a specific place, perhaps, due to the different fixing agents during impregnation method (Hanselaar et al, 1992). The usage of different fixing agents with different fixation methods shows some varieties about chromogen intensity in the ionic hydrogen concentration and the hydrolise time in Feulgen's reaction (Böhm;Böhm et al, 1968) that could interfere in the condensation of chromatin's spatial distribution and, apart from tracking the DNA, does the same with proteins (Stedman & Stedman, 1950), Umayahara et al (2002). Meanwhile, uncharacteristic DNA distributions, for some authors, in comparison with results from the epithelial of the uterine cervix cytrometry and its DNA, aren't an early malignity diagnostic method, Haroske et al (2001), Ishikawa et al (2002).…”

The Dna Content of Spermatozoa From Different Strains of Mice

“…It has been shown that the Feulgen-DNA contents of ejaculated spermatozoa are lower than those of epididymal or testicular spermatozoa (Bouters, Esnault, Salisbury & Ortavant, 1967). In the present study, all the spermatozoa were taken from the same region of the genital tract and were processed for the Feulgen reaction by methods prescribed to eliminate proportionality errors (Böhm et al, 1968). If the mode of chromatin condensation within the sperm head differs between strains, it is not related to nuclear size.…”