Proportion of the CD19-Positive and CD19-Negative Lymphocytes and Monocytes within the Peripheral Blood Mononuclear Cell Set Is Characteristic for Rheumatoid Arthritis

Abstract

Background and objectives: Composition of the peripheral blood (PB) cell populations and their activation state reflect the immune status of a patient. Rheumatoid arthritis (RA) is characterized by abnormal B- and T-cell functions. The objective of this study was to assess the profiles of the PB mononuclear cell (PBMC) populations in patients with rheumatoid and osteoarthritis (OA) in comparison with healthy control (HC) subjects in order to evaluate the PBMC profiles as a potential diagnostic characteristic in RA. The second aim was to assess the CCR1 and CCR2 expression on PB lymphocytes and correlate it with the plasma levels of matrix metallopeptidase 9 (MMP-9), IL-17F, TNF-α, IL-6, and IL-10. Materials and Methods: The frequency and phenotype, including CCR1 and CCR2, of the PBMC populations (monocytes, CD19+B cells, and T/NK lymphocytes) in RA (n = 15) and OA (n = 10) patients and HC (n = 12) were analyzed by five-color flow cytometry. DNA of the viruses, HHV-6, HHV-7, and B19, in the whole blood and cell-free plasma, were assessed by nested-polymerase chain reaction (PCR). Results: Active persistent or acute infections, caused by HHV-6, HHV-7, or B19, were not detected in patients of this study. Both CCR1 and CCR2 were determined on the PB B and T/NK lymphocytes in several RA and OA patients and HCs. However, in patients, the frequency of the CCR1-positive T/NK lymphocytes showed a weak negative correlation with the IL-10 level, while the frequency of the CCR2-positive B cells correlated positively with the level of IL-6. Statistically significant differences in the proportions of the CD19-positive and CD19-negative lymphocyte and monocyte subsets within the PBMC set were determined between RA and OA patients and HC adults. Conclusions: We have shown in our pilot study with rather small cohorts of patients that the PBMC-population profiles were very consistent, and statistically significantly differed between RA and OA patients and HC subjects.

Longitudinal DNA methylation dynamics as a practical indicator in clinical epigenetics

“…To reduce the possible effects of different cell types, the CpGs whose longitudinal DNA methylation changes were associated with cell types were excluded. However, only six major cell types were considered in this study, and we cannot account for subtypes of blood cells, which can change along with the onset of immune disorders [ 57 ]. Therefore, the individual results obtained from each PBMC and monocyte sample were not discussed.…”

Expression of the Chemokine Receptor CCR1 in Burkitt Lymphoma Cell Lines Is Linked to the CD10-Negative Cell Phenotype and Co-Expression of the EBV Latent Genes EBNA2, LMP1, and LMP2

“…We did not detect CCR2 on the cell surface of the LCL cells nor BL cells after serum deprivation. Nevertheless, using the same mouse monoclonal anti-CD192-Alexa Fluor 647 antibodies, we demonstrated the cell-surface expression of CCR2 on monocytes and B lymphocytes in PB of patients with rheumatoid arthritis [ 24 ] and patients with chronic lymphocytic leukemia [ 25 ].…”

Identifying Key Genes and Functionally Enriched Pathways in B cells from Peripheral Blood of Rheumatoid Arthritis Patients by Bioinformatics Analysis and Validation

“…CC motif chemokine receptor-2 (CCR2) on B cells can participate in the body's in ammatory response by binding to monocyte chemoattractant protein-1 [22]. In the RA patients' peripheral blood, ratio of CCR2 + B cells correlates positively with the level of IL-6 [23]. As another important chemokine receptor, CX3CR1 has been studied extensively on T cells and monocytes in RA [15,17].…”