Proportion of extended-spectrum ß-lactamase-producing Enterobacteriaceae in community setting in Ngaoundere, Cameroon

Lonchel, Carine Magoué; Meex, Cécile; Gangoué-Piéboji, Joseph; Boreux, Raphaël; Assoumou, Marie-Claire Okomo; Melin, Pierrette; Mol, Patrick De

doi:10.1186/1471-2334-12-53

Cited by 77 publications

(74 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The evolution of antibiotic resistance is a continuous threat to human health worldwide [4]. E. coli may act as a reservoir of genes coding for antibiotic resistance and may also be responsible for a group of endogenous infections.…”

Section: Discussionmentioning

confidence: 99%

“…Group-specific primers used in the study [5]. Much lower rates were obtained in different regions of the world: 12.3% in Saudi Arabia [15], 11% in Egypt [4], 7.3% in Tunisia [16], and 1.8% in Portugal [3]. All these studies investigated the prevalence of ESBL-producing Enterobacteriaceae in community settings rather than in HCWs, which may explain the lower rates.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have evaluated fecal carriage of ESBL-producing isolates from the stools of healthy individuals in community settings [3][4][5], but aside from one recent study [6], they did not specifically investigate ESBLs in healthcare workers (HCWs).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Fecal carriage of extended-spectrum β-lactamase- and AmpC- producing Escherichia coli among healthcare workers

Bassyouni

Gaber

Wegdan

2015

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: Commensal E. coli can be considered a reservoir of genes coding for antibiotic resistance that may be transmitted in hospitals by healthcare workers (HCWs). This study aimed to determine the fecal carriage rate of extended-spectrum β-lactamase (ESBL)-producing E. coli among HCWs. Methodology: Stool samples were collected from 200 HCWs. Phenotypic screening for ESBL and AmpC β-lactamases was performed using disk diffusion and minimum inhibitory concentration methods followed by the combined disks test and double synergy differential test for confirmation. Multiplex polymerase chain reaction (PCR) was used to detect bla SHV , bla TEM , bla CTX-M , and CIT groups for AmpC genes. Results: Of 200 E. coli isolates, 100% were susceptible to imipenem, and 59 (29.5%) were resistant to one or more third-generation cephalosporins. By molecular analysis, 21% (42/200) were colonized by ESBL-producing E. coli, and 3% (6/200) were colonized by AmpCproducing E. coli. The bla SHV gene was the predominant ESBL gene, detected in 81.8% of the resistant E. coli isolates. Conclusions: These findings highlight the increase in fecal carriage of E. coli carrying ESBL and AmpC genes among HCWs, which may be one of the causes of the spread of ESBL-producing bacteria in hospitals and requires sound infection control measures. This is the first study of the fecal carriage rate of E. coli carrying AmpC genes in HCWs.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Fecal carriage of extended-spectrum β-lactamase- and AmpC- producing Escherichia coli among healthcare workers

Bassyouni

Gaber

Wegdan

2015

J Infect Dev Ctries

View full text Add to dashboard Cite

show abstract

“…Indeed, ESBL-producing Enterobacteriaceae have been associated with serious nosocomial infection outbreaks that lead to prolonged hospital stay, increased morbidity and mortality, and consequently increased healthcare associated costs with limited therapeutic options (Pitout, 2010). Increasing rate of community-acquired infections caused by ESBL-producing Enterobacteriaceae has, however, been recently reported (Lonchel et al, 2012), representing a potential reservoir for ESBL producers.…”

Section: Esbl-mentioning

confidence: 99%

Extended-spectrum beta-lactamase- and carbapenemase-producing Enterobacteriaceae clinical isolates in a Senegalese teaching hospital: A cross sectional study

Camara¹,

Mane²,

Ba-Diallo³

et al. 2017

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Extended spectrum β-lactamase (ESBL) and carbapenemase-producing Enterobacteriaceae (CPE) have been increasingly reported worldwide. The objective of this study is to determine the prevalence of these multidrug-resistant strains in a major university teaching hospital in Dakar, Senegal. A total of 1205 Enterobacteriaceae stains were tested for ESBL and carbapenemase production. Antibiotics susceptibility test was performed with disk diffusion method. ESBL was detected using a double-disk synergy method. Carbapenemase production was detected with ertapenem 10 µg disk charge. The overall prevalence of ESBL-and carbapenemase-producing Enterobacteriaceae was 26.2 (316/1205) and 5.1% (62/1205), respectively. Interestingly, 3.8% of these pathogens were both ESBL-carbapenemase producers. Among the Enterobacteriaceae ESBL positive, Escherichia coli (45.2%, 143/316), Klebsiella pneumoniae (26.3%, 83/316), Enterobacter cloacae (12.7%, 40/316), and Proteus vulgaris (9.2%, 29/316) were the most prevalent. These strains were mainly isolated from urine (56.6%) and pus (22.7%) specimen. The most prevalent CPEs were E. coli (45.2%, 28/62), K. pneumoniae (27.4%, 17/62), and E. cloacae (16.1%, 10/62), particularly isolated from urine (58%) and pus (19.3%). The majority of these MDR strains were isolated from patients hospitalized in urology (32.4%), surgery (27.7%), internal medicine (18.5%), and intensive care units (10%). ESBL-producing Enterobacteriaceae remain highly susceptible to fosfomycin (94.1%), amikacin (92.5%) and ertapenem (88.6%), while carbapenemase producers were fully susceptible to amikacin (100%), and to a lesser extent, fosfomycin (66.7%) and colistin (60%). This study revealed increasing prevalence of ESBL-and carbapenemase-producing Enterobacteriaceae with limited therapeutic options, suggesting a need for continuous multi-drug resistant (MDR) surveillance patterns particularly in hospital settings.

show abstract

“…[2,[6][7][8] Few studies have been done on ESBL however they only looked at intestinal carriage. [9][10][11] The prevalence of CTX-M in E. coli strains responsible for community-acquired UTI in Cameroon or Central Africa has not been documented. The aim of our study was to determine the prevalence of bla CTX-M-type in E. coli strains in community-acquired urinary tract infections in women as well as its risk factors in Cameroon.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of CTX-M beta-Lactamases in Escherichia coli from community-acquired urinary tract infections and associated risk factors among women in Cameroon

Djuikoue

Njajou

Kamga

et al. 2017

JER

View full text Add to dashboard Cite

Background: In recent years, a worldwide dissemination of CTX-M beta-lactamase-type in Escherichia coli strains isolates from community-acquired urinary tract infections (CA-UTI) has been observed. However little is known on the prevalence and risk factors of this global threat in developing countries. Objective: The aim of this study was to study the prevalence and risk factors for CA-UTI in Yaoundé, Cameroon. Methods: Eighty six patients with urinary E.coli infection recruited from 10 health structures in the town of Yaoundé, Cameroon. After taking the first urine, faeces were collected from the patients for the study of the intestinal flora. The sample collection of faeces was done on a selected gel of enterobacteria resistant to third generation of cephalosporin. The molecular typing of extended-spectrum β-lactamase (ESBL) was carried out. Results: Eighty-six strains of E. coli from 86 patients were included. We found that 39 (45.3%) strains produced an extendedspectrum beta-lactamase. Among risk factors, previous use of antibiotic and the dry season were associated with the presence of an ESBL-producing strain in the urine. All ESBL were identified as CTX-M. The production of CTX-M was found to be significantly associated with resistance to fluoroquinolones, aminoglycosides and to the association of trimethoprim-sulfamethazole. Conclusions: The prevalence of CTX-M ESBL in Yaoundé, Cameroon, provides new evidence on the global dissemination of CTX-M and the extent of this phenomenon in developing countries.

show abstract

Proportion of extended-spectrum ß-lactamase-producing Enterobacteriaceae in community setting in Ngaoundere, Cameroon

Cited by 77 publications

References 30 publications

Fecal carriage of extended-spectrum β-lactamase- and AmpC- producing Escherichia coli among healthcare workers

Fecal carriage of extended-spectrum β-lactamase- and AmpC- producing Escherichia coli among healthcare workers

Extended-spectrum beta-lactamase- and carbapenemase-producing Enterobacteriaceae clinical isolates in a Senegalese teaching hospital: A cross sectional study

Prevalence of CTX-M beta-Lactamases in Escherichia coli from community-acquired urinary tract infections and associated risk factors among women in Cameroon

Contact Info

Product

Resources

About