Propolis Standardized Extract (EPP-AF®), an Innovative Chemically and Biologically Reproducible Pharmaceutical Compound for Treating Wounds

Abstract

The aim of this study was to develop a formulation, containing the propolis standardized extract (EPP-AF®), which can assist in the healing of skin lesions. To achieve this objective the antimicrobial activity and chemical composition of the propolis extract was determined. The final product was subjected to in vitro and in vivo pre-clinical evaluation. The broth macrodilution method was used to determine the antimicrobial activity of the extracts and formulations against the microorganisms most commonly found in burns, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus and Staphylococcus epidermidis. Wistar rats with puncture wounded skin were used to evaluate the wound healing properties of propolis. The results of chemical and biological characterization demonstrated the batch-to-batch reproducibility of the standardized extract which is an unprecedented result. The antimicrobial and wound healing activity of the pharmaceutical studied showed the best results when samples contain 3.6% propolis, suggesting that this is the most promising composition.

In VitroAntimicrobial Activity of Ethanolic Extract of Polish Propolis against Biofilm FormingStaphylococcus epidermidisStrains

“…Berretta et al [51] concluded that microorganisms, such as Pseudomonas aeruginosa , Klebsiella pneumoniae , Escherichia coli , S. aureus, and S. epidermidis are the most frequently isolated from injuries and burn wounds. The authors tested antimicrobial activity of the different extracts and propolis formulations against the above-mentioned microorganisms and concluded that antimicrobial and wound-healing activity showed the best results when applying pharmaceutics containing 3.6% addition of propolis.…”

Quantificação de fenóis, flavonoides totais e atividades farmacológicas de geoprópolis de Plebeia aff. Flavocincta do Rio Grande do Norte

“…flavocincta, no qual foi constatado pela redução significativa da densidade de célu-las polimorfonucleares, aumento significativo da contagem dos fibroblastos e fibras colágenas nos animais do GE. Os resultados das análises histológicas realizadas no presente estudo foram semelhantes aos obtidos por Berretta et al (2012), que observaram diminuição do recrutamento de células inflamatórias e aumento da produção de colágeno em feridas cutâneas experimentais em ratos tratados com gel de própolis verde, após o terceiro dia de tratamento. Estudo realizado por Abreu et al (2012) demonstraram que a aplicação tópica do extrato de própolis a 30% foi capaz de promover a fibroplasia, a re-epitelização e aceleração da cicatrização, quando comparada a um grupo controle.…”

Bacterial Cellulose

“…Propolis is a resinous mixture that honey bees collect from nature and use as sealant in the hive. It is known to present antifungal, antiviral, antioxidant, anti-inflammatory and antibacterial activity [31,32]. BCpropolis membranes have been prepared and have been shown to favor tissue repair in less time compared with pristine BC.…”