Propolis Potential Toward the Amount of Limphoblast and Spleen Diameter of Male Mice (Mus musculus)

Abstract

The aim of this research was to find out the effect of propolis in the amount of lymphoblast and the correlation between the treatment of propoliswith spleen diameter each of white male mice (Mus musculus). This research used 25 male white mices which are 12 weeks old has 25-35 gram of body weight. They were divided randomly into five groups. (P0) as control was given 0,5 ml aquades/day, and other group were given propolis for 0.4 mg/0.03kg/day (P1), 0.8 mg/0.03kg/day (P2), 1.6 mg/0.03kg/day (P3), and 3.2 mg/0.03kg/day (P4). After two weeks treatment, 25 mices were sacrificed their spleen were then used in histological preparation with HE staining. The data were analyzed by ANOVA method based on Completely Randomized Design and further analyzed by Duncan's Multiple Range Test. The result from statistical analysis showed that treatment with propolis increased the amount of lymphoblast also the spleen diameter of white male mice (p<0,05) and show positive correlation amount of lymphoblast and white pulp spleen diameter.