Propolis microparticles produced with pea protein: Characterization and evaluation of antioxidant and antimicrobial activities

Jansen-Alves, Cristina; Maia, Darla Silveira Volcan; Krumreich, Fernanda Döring; Crizel-Cardoso, Michele M.; Fioravante, Júlia Borin; Silva, Wladimir Padilha da; Borges, Caroline Dellinghausen; Zambiazi, Rui Carlos

doi:10.1016/j.foodhyd.2018.09.004

Cited by 68 publications

(43 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Andrade et al [66], Machado et al [27], Szliszka et al [126], and Jansen-Alves et al [127] found kaempferol in samples of Brazilian green propolis, and it was considered to be one of the main constituents of this type of propolis. In the current study, significant amounts of kaempferol were identified in the red propolis extracts from northeastern Brazil (Fig 3).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of the antioxidant profile and cytotoxic activity of red propolis extracts from different regions of northeastern Brazil obtained by conventional and ultrasound-assisted extraction

et al. 2019

View full text Add to dashboard Cite

Propolis is a complex mixture of resinous and balsamic material collected from the exudates of plants, shoots, and leaves by bees. This study evaluated red propolis extracts obtained by conventional (ethanolic) extraction and ultrasound-assisted extraction of six samples from different regions of northeastern Brazil. The total phenolic compounds and flavonoids, in vitro antioxidant activity, concentration of formononetin and kaempferol and the cytotoxicity against four human tumor cell lines were determined for all twelve obtained extracts. Significant variations in the levels of the investigated compounds were identified in the red propolis extracts, confirming that the chemical composition varied according to the sampling region. The extraction method used also influenced the resulting propolis compounds. The highest concentration of the compounds of interest and the highest in vitro antioxidant activity were exhibited by the extracts obtained from samples from state of Alagoas. Formononetin and kaempferol were identified in all samples. The highest formononetin concentrations were identified in extracts obtained by ultrasound, thus indicating a greater selectivity for the extraction of this compound by this method. Regarding cytotoxic activity, for the HCT-116 line, all of the extracts showed an inhibition of greater than 90%, whereas for the HL-60 and PC3 lines, the minimum identified was 80%. In general, there was no significant difference (p>0.05) in the antiproliferative potential when comparing the extraction methods. The results showed that the composition of Brazilian red propolis varies significantly depending on the geographical origin and that the method used influences the resulting compounds that are present in propolis. However, regardless of the geographical origin and the extraction method used, all the red propolis samples studied presented great biological potential and high antioxidant activity. Furthermore, the ultrasound-assisted method can be efficiently applied to obtain extracts of red propolis more quickly and with high concentration of biomarkers of interest.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Important biological effects have been reported for kaempferol in recent studies [127,130,131]. In addition to propolis, kaempferol is a flavonoid found in botanical products that are commonly used in traditional medicines, such as Ginkgo biloba [132,133] and Sophora japonica [134–136].…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the antioxidant profile and cytotoxic activity of red propolis extracts from different regions of northeastern Brazil obtained by conventional and ultrasound-assisted extraction

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The morphology of the particles is shown in Figure 7, showing spray‐dried powders (Figure 7a,b) with different sizes and apparently without cracks, characteristic of the powders obtained by this drying method (Abrahão et al, 2019; Kuck & Noreña, 2016). The absence of cracks is an essential aspect which enables adequate protection, ensuring the retention of the encapsulated compound, and lower gas permeability (Jansen‐Alves et al, 2019; Rocha, Fávaro‐Trindade, & Grosso, 2012).…”

Section: Resultsmentioning

confidence: 99%

“…The roughness presented is usually attributed to the retraction occurring after the rapid water evaporation during the drying process in the spray dryer, with subsequent cooling of the particles (Jansen‐Alves et al, 2019). Furthermore, this characteristic is commonly exhibited in spray‐dried powders when encapsulated with proteins (Fäldt & Bergenståhl, 1994), which can be indicated by the morphology presented by hydrolyzed collagen powder (Figure 7a), possibly due to the presence of polymeric structures characterized as collagen fibers (Valencia et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

Microwave‐assisted extraction of bioactive compounds fromAraucaria angustifoliabracts followed by encapsulation

Dorneles

Noreña

2020

J Food Process Preserv

View full text Add to dashboard Cite

The aqueous extract of Araucaria angustifolia bracts was obtained by microwave‐assisted extraction and encapsulated by spray‐drying and freeze‐drying using pectin/hydrolyzed collagen (PEC‐HC) and polydextrose/partially hydrolyzed guar gum (PD‐PHGG) dispersions, added to the extract in the proportions of 0.25:0.75:9 and 0.5:0.5:9 (wt/wt/wt), respectively. The powders encapsulated with the PD‐PHGG mixture by spray‐drying and freeze‐drying showed the highest retention rates for total phenolic compounds (80.57% and 89.94%) and solubility (98.00 and 97.69 g 100 g−1), respectively. The scanning electron microscopy (SEM) images presented spherical forms on the spray‐dried powders, while the freeze‐dried powders exhibited irregular forms. The results obtained by FTIR suggested that the encapsulation occurred in all samples by physical interactions, whereas the thermogravimetric analysis showed that the PEC‐HC powders demonstrated greater thermal stability. Accelerated storage tests indicated that the PD‐PHGG freeze‐dried powders had lower decrease in the antioxidant capacity. Practical applications The industrial use of Araucaria angustifolia bracts (sterile seeds) is currently considered a type of waste. However, the bracts present bioactive compounds, including condensed tannins, considered among phenolic compounds, the most important natural antioxidants. The phenolic compounds of the bracts were extracted by microwave, using exclusively water as a solvent. The application of green technologies is an interesting alternative for the food industry since the use of organic solvents is completely replaced, as well shorter exposure times to high temperatures are obtained and, consequently, lower energy expenditure and lower antioxidant capacity loss. For the encapsulation of phenolic compounds, dietary fibers and protein were used as wall materials, which resulted in a powder containing natural antioxidants, fibers, and essential amino acids, which can be applied in several food formulations.

show abstract

“…Research on propolis standardization together with new methods and approaches for propolis addition into different types of foods (e.g. via microencapsulation; Jansen-Alves et al., 2019) could lead to its widespread application as a natural food preservative.…”

Section: Resultsmentioning

confidence: 99%

Synergistic, bacteriostatic effect of propolis and glycerol against Listeria monocytogenes in chocolate milk under refrigerated storage

Michailidis

Thamnopoulos

Fletouris

et al. 2020

Food sci. technol. int.

View full text Add to dashboard Cite

Propolis ethanolic extracts, with or without glycerol, were added into pasteurized, non-fat chocolate milk, which was artificially contaminated with Listeria monocytogenes. The addition of propolis ethanolic extracts dissolved into glycerol led to a definite anti-listerial effect in milk stored at 4 ℃, with both propolis concentrations tested (2 or 4 mg of dry propolis ethanolic extract per milliliter of chocolate milk) leading to inhibition of L. monocytogenes growth throughout 20 days of storage. The combined addition of propolis ethanolic extracts with glycerol was also effective in significantly reducing the rate of growth of L. monocytogenes in chocolate milk stored under improper (10 ℃) refrigeration storage conditions (more than five-fold increase in the generation time of L. monocytogenes compared to control trials). Finally, the combined addition of a deodorized propolis ethanolic extract with glycerol resulted in a significant anti-listerial effect upon storage of contaminated milk at 4 ℃ (more than three-fold increase in the generation time of L. monocytogenes compared to controls) and in a smaller anti-listerial effect upon milk storage at 10 ℃ (two-fold increase in the generation time of the pathogen compared to controls). Of note, chocolate milk containing deodorized propolis ethanolic extract and glycerol received a positive consumer acceptability score on the nine-point hedonic scale (median acceptability score of “7”). Hence, propolis may possess a promising role as a natural anti-listerial preservative in dairy drinks.

show abstract

Propolis microparticles produced with pea protein: Characterization and evaluation of antioxidant and antimicrobial activities

Cited by 68 publications

References 44 publications

Evaluation of the antioxidant profile and cytotoxic activity of red propolis extracts from different regions of northeastern Brazil obtained by conventional and ultrasound-assisted extraction

Evaluation of the antioxidant profile and cytotoxic activity of red propolis extracts from different regions of northeastern Brazil obtained by conventional and ultrasound-assisted extraction

Microwave‐assisted extraction of bioactive compounds fromAraucaria angustifoliabracts followed by encapsulation

Synergistic, bacteriostatic effect of propolis and glycerol against Listeria monocytogenes in chocolate milk under refrigerated storage

Contact Info

Product

Resources

About