Propolis-induced genotoxicity and antigenotoxicity in Chinese hamster ovary cells

Tavares, Denise Crispim; Barcelos, Gustavo Rafael Mazzaron; Silva, Lívia Ferreira; Tonin, Conception Cortez Chacon; Bastos, Jairo Kenupp

doi:10.1016/j.tiv.2006.02.009

Cited by 63 publications

(44 citation statements)

References 24 publications

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“…It has been reported that this plant is a source of resin for Brazilian propolis (green propolis), because of the similarity of their chemical constituents (Park et al, 2004). In vitro toxicological studies of green propolis revealed that low concentrations show antimutagenicity and high concentrations show mutagenicity effects (Tavares et al, 2006). In vivo analysis confirmed that high doses produced mutagenic effects (Pereira et al, 2008).…”

Section: Introductionmentioning

confidence: 91%

Lack of clastogenic/genotoxic effects of Baccharis dracunculifolia extract on Swiss mouse peripheral blood cells

Andrade¹,

Perazzo²,

Maistro³

2008

Genet. Mol. Res.

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ABSTRACT. Baccharis dracunculifolia De Candole (DC) (Asteraceae) is indigenous throughout southeastern Latin America and is used by local people in traditional medicine. This plant is known to be the source of resin for the highly valued Brazilian green propolis. As no information is available on the safety of high doses of B. dracunculifolia extract, we evaluated the mutagenic potential of high doses of this plant extract in vivo on peripheral blood cells of Swiss mice using the comet assay and the micronucleus test. The extract was administered by gavage at doses of 1000, 1500 and 2000 mg/kg body weight. Peripheral blood cell samples were collected 4 and 24 h after treatment for the comet assay (genotoxicity assay), and at 48 and 72 h for the micronucleus test (clastogenicity assay). The B. dracunculifolia extract was devoid of clastogenic/genotoxic activity at all doses.

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Section: Introductionmentioning

confidence: 91%

Lack of clastogenic/genotoxic effects of Baccharis dracunculifolia extract on Swiss mouse peripheral blood cells

Andrade¹,

Perazzo²,

Maistro³

2008

Genet. Mol. Res.

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“…Tavares et al 14 The tests also showed that celecoxib, propolis and L-lysine (at two times higher dose) initiated in the 32nd week were also able to significantly reduce cellular genotoxic damage when compared with the controls that were subjected to BBN. It should be recalled …”

Section: Discussionmentioning

confidence: 94%

Potential chemoprotective effects of green propolis, L-lysine and celecoxib on bone marrow cells and peripheral blood lymphocytes of Wistar rats subjected to bladder chemical carcinogenesis

et al. 2014

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PURPOSE:To evaluate the genotoxicity of propolis and L-lysine, as well as their effects on the possible cellular damage in erythroblasts (bone marrow) and leukocytes (peripheral blood) caused by the carcinogen BBN (n -butyl -n {4 -hydroxybutyl} nitrosamine) in rats subjected to bladder carcinogenesis and treated with green propolis and L-lysine. METHODS:One hundred and twenty five rats were distributed into the following groups: I, IIA, IIB, III, K, L M N, X, XI, XII and XIII. Groups I to X received BBN in drinking water for 14 weeks (wks). Group I was treated with intragastric (ig) propolis at 150 mg/ kg body weight, for 44 wks, beginning 30 days before start of BBN. Groups IIA and III were treated with propolis (150 mg/kg), for 40 wks, subcutaneous (sc) and ig, respectively, beginning simultaneously with BBN. On the 32nd wk, the animals of groups L, M and N were treated ig with L-lysine (300 mg/kg), celecoxib (30 mg/kg) and propolis (300 mg/kg), respectively, up to the 40th wk. The groups that received only BBN (IIB and K) were treated with water, sc and orally, respectively, for 40 wks. Groups XI, XII and XIII received respectively propolis (150 mg/kg), L-lysine (150 mg/kg) and water ig for 40 wks. After 40 wks, the surviving animals were anesthetized and subjected to femoral bone marrow aspiration and blood collection from the aorta, for CA and MNT, respectively, for investigation of genotoxicity.RESULTS: Groups IIB and K, which received only BBN and water, showed the greatest DNA damage in peripheral leukocytes (CA) and largest number of micronuclei in bone marrow erythrocytes (MNT) in relation to all other groups that received BBN and lysine and/ or propolis (p<0.001). CONCLUSIONS:Both propolis and L-lysine are effective in protecting against genotoxicity, as well not being genotoxic themselves toward the cells evaluated, at the doses and times administered and according to the two tests utilized.

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“…The melatonin has also radioprotector effects with a maximum protectable fraction of 68% at 2 mM [6]. Simultaneous treatment with propolis and a chemotherapeutic agent doxorubicin led to a reduction of 64.3% in the frequency of chromosome aberrations [27]. However, most of these products produce serious side effects or cytotoxicity, and some of them are considered to be toxic at the concentrations required to reach radioprotection.…”

Section: Discussionmentioning

confidence: 99%

Combined effect of viscous dissipation and thermal radiation on fluid flows over a non-linearly stretched permeable wall

Cortell

2011

Meccanica

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Radioprotection with natural products may be relevant to the mitigation of ionizing radiation-induced damage in mammalian systems; in this sense, propolis extracts have shown effects such as antioxidant, antitumoral, anti-inflammatory, and immunostimulant. We report for the first time a cytogenetic study to evaluate the radioprotective effect, in vitro, of propolis against radiation-induced chromosomal damage. Lymphocytes were cultured with increasing concentrations of ethanol extract of propolis (EEP), including 20, 40, 120, 250, 500, 750, 1000, and 2000 μg mL −1 and then exposed to 2 Gy γ-rays. A significant and concentration-dependent decrease is observed in the frequency of chromosome aberrations in samples treated with EEP. The protection against the formation of dicentrics was concentration-dependent, with a maximum protection at 120 μg mL −1 of EEP. The observed frequency of dicentrics is described as negative exponential function, indicating that the maximum protectible fraction of dicentrics is approximately 44%. Free radical scavenging and antioxidant activities are the mechanisms that these substances use to protect cells from ionizing radiation.

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Propolis-induced genotoxicity and antigenotoxicity in Chinese hamster ovary cells

Cited by 63 publications

References 24 publications

Lack of clastogenic/genotoxic effects of Baccharis dracunculifolia extract on Swiss mouse peripheral blood cells

Lack of clastogenic/genotoxic effects of Baccharis dracunculifolia extract on Swiss mouse peripheral blood cells

Potential chemoprotective effects of green propolis, L-lysine and celecoxib on bone marrow cells and peripheral blood lymphocytes of Wistar rats subjected to bladder chemical carcinogenesis

Combined effect of viscous dissipation and thermal radiation on fluid flows over a non-linearly stretched permeable wall

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