Propolis effects on pro-inflammatory cytokine production and Toll-like receptor 2 and 4 expression in stressed mice

Pagliarone, Ana Carolina; Orsatti, Cláudio Lera; Búfalo, Michelle Cristiane; Missima, Fabiane; Bachiega, Tatiana Fernanda; Júnior, João Pessoa Araújo; Sforcin, José Maurício

doi:10.1016/j.intimp.2009.08.005

Cited by 33 publications

(23 citation statements)

References 40 publications

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“…In acutely stressed mice, Brazilian green propolis (200 mg/kg for 3 days) restored TLR-2 and TLR-4 expression (Pagliarone et al, 2009b), contributing to the recognition of microorganisms during stressful conditions, and increased IL-4 production, favoring humoral immune response (Pagliarone et al, 2009a). In chronically stressed mice, Brazilian green propolis treatment (200 mg/kg for 7 days) potentiated H 2 O 2 generation by macrophages and counteracted the alterations found in the spleen (Missima and Sforcin, 2008).…”

Section: Propolis Immunomodulatory Actionmentioning

confidence: 95%

Propolis: Is there a potential for the development of new drugs?

Sforcin

Bankova

2011

Journal of Ethnopharmacology

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697

574

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Section: Propolis Immunomodulatory Actionmentioning

confidence: 95%

Propolis: Is there a potential for the development of new drugs?

Sforcin

Bankova

2011

Journal of Ethnopharmacology

Self Cite

697

574

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“…9 The aim of the present study was to assess the initial response of the dental pulp in rats, following pulpotomy and pulp capping with substances containing green propolis.…”

Section: Green Propolismentioning

confidence: 99%

Inflammatory effect of green propolis on dental pulp in rats

Esmeraldo

Carvalho

et al. 2013

Braz. oral res.

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“…The smallest cDNA standard dilution was assigned a relative value of 100, and the other three dilutions were correspondingly assigned values of 20, 4.0, and 0.8. The relative concentrations of hMLH1 and RASSF1A were determined by the relative standard curve method after normalization with β-actin with SDS version 1.2.3 software (Sequence Detection Systems 1.2.3, 7500 Real-Time PCR Systems, Applied Biosystems, Foster City, CA, USA) (Pagliarone et al, 2009;Orsatti et al, 2010).…”

Section: Standard Curve and Data Analysismentioning

confidence: 99%

No Relationship between the Amount of DNA Damage and the Level of hMLH1 and RASSF1A Gene Expression in Bladder Cancer Cells Treated with Cisplatin and Gemcitabine

Camargo

Silva²,

Gobette³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Tumor response to antineoplastic drugs is not always predictable. This is also true for bladder carcinoma, a highly recurrent neoplasia. Currently, the combination of cisplatin and gemcitabine is well accepted as a standard protocol for treating bladder carcinoma. However, in some cases, this treatment protocol causes harmful side effects. Therefore, we investigated the roles of the genes TP53, RASSF1A (a tumor suppressor gene) and hMLH1 (a gene involved in the mismatch repair pathway) in cell susceptibility to cisplatin/gemcitabine treatment. Two bladder transitional carcinoma cell (TCC) lines, RT4 (wild-type TP53) and 5637 (mutated TP53), were used in this study. First, we evaluated whether the genotoxic potential of cisplatin/gemcitabine was dependent on TP53 status. Then, we evaluated whether the two antineoplastic drugs modulated RASSF1A and hMLH1 expression in the two cell lines. Increased DNA damage was observed in both cell lines after treatment with cisplatin or gemcitabine and with the two drugs simultaneously, as depicted by the comet assay. A lack of RASSF1A expression and hypermethylation of its promoter were observed before and after treatment in both cell lines. On the other hand, hMLH1 downregulation, unrelated to methylation status, was observed in RT4 cells after treatment with cisplatin or with cisplatin and gemcitabine simultaneously (wild-type TP53); in 5637 cells, hMLH1 was upregulated only after treatment with gemcitabine. In conclusion, the three treatment protocols were genotoxic, independent of TP53 status. However, cisplatin was the most effective, causing the highest level of DNA damage in both wild-type and mutated TP53 cells. Gemcitabine was the least genotoxic agent in both cell lines. Furthermore, no relationship was observed between the amount of DNA damage and the level of hMLH1 and RASSF1A expression. Therefore, other alternative pathways might be involved in cisplatin and gemcitabine genotoxicity in these two bladder cancer cell lines.

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Propolis effects on pro-inflammatory cytokine production and Toll-like receptor 2 and 4 expression in stressed mice

Cited by 33 publications

References 40 publications

Propolis: Is there a potential for the development of new drugs?

Propolis: Is there a potential for the development of new drugs?

Inflammatory effect of green propolis on dental pulp in rats

No Relationship between the Amount of DNA Damage and the Level of hMLH1 and RASSF1A Gene Expression in Bladder Cancer Cells Treated with Cisplatin and Gemcitabine

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