Propolis: A Potential Natural Product to Fight
            <i>Candida</i>
            Species Infections

Tobaldini-Valério, Flávia; Mendonca, Patricia; Rosseto, Hélen Cássia; Bruschi, Marcos Luciano; Henriques, Mariana; Negri, Melyssa; Silva, Sónia; Svidzinski, Terezinha Inez Estivalet

doi:10.2217/fmb-2015-0016

Cited by 56 publications

(36 citation statements)

References 51 publications

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“…In this study, a greater reduction in C. albicans biofilm formation was observed at all concentrations compared to the biofilms produced by C. parapsilosis and C. tropicalis , and the reduction of biofilm in C. parapsilosis was significantly higher from the concentration of C. parapsilosis . 25 μg/mL and C. tropicalis at 250 μg/mL, corroborating the work of Tobaldini-Valerio et al (21) who also observed a greater biofilm reduction (~ 3.5 log) in C. albicans , followed by C. parapsilosis and C. tropicalis , with a reduction of approximately 2.8 and 2 log, respectively, at all Propolis Extract concentrations. tested.…”

Section: Discussionsupporting

confidence: 88%

Highly Efficient Antibiofilm and Antifungal Activity of Green Propolis againstCandidaspecies in dentistry material

Bezerra

Borges

Alves

et al. 2020

Preprint

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31Background: This study evaluated the influence of green propolis' extract on the 32 adhesion and biofilm formation of Candida species on dentistry material. Methods: 33 Phytochemical analysis of green propolis' extract was performed by High Performance 34 Liquid Chromatography. Adhesion was quantified in a Neubauer chamber, counting the 35 number of yeast cells adhered to the fragments; Biofilm formation was determined by 36 counting the number of colony forming units (CFU). The intensity of biofilm formation 37 adhesion was classified as negative, weak, moderate, strong and very strong. Fifteen 38 compounds were identified in green propolis extract, mainly flavonoids. Results: All 39 strains were able to adhere and form biofilm on the surface of the orthodontic materials 40 studied. In steel and resin, the adhesion intensity of the yeast cells was weak at all 41 incubation times, except for C. parapsilosis and C. tropicalis which at 12hs showed 42 moderate intensity. Regarding biofilm formation (24 and 48 hours), it was observed in 43 the steel that C. albicans had moderate intensity at 24 and 48 hours; C. parapsilosis at 44 24 and 48 hours had very strong intensity; C. tropicalis at 24 hours had strong intensity 45 and at 48 hours very strong. While in the resin, all species at 24 and 48 hours had strong 46 intensity, except for C. tropicalis which at 48 hours had very strong intensity. Green 47 propolis extract showed antifungal activity and was able to inhibit both adhesion and 48 biofilm formation at 2.5 µg/mL. Conclusions: This study reinforces the idea that green 49 propolis has antifungal activity and interferes with virulence factors of Candida species. 50 Keywords: Green Propolis; Candida sp, Biofilm; Dentistry material. 51 52 53 54 55 56 57 58 3 59 BACKGROUND 60In recent years the use of orthodontic materials has increased for aesthetic, surgical 61 and biofunctional purposes. Polymers, ceramics, composites, resin, steels and their 62 alloys are used in the manufacture of dental prostheses, screws and orthodontic 63 appliances and when implanted in the oral cavity they are exposed to colonization and 64 biofilm formation by microorganisms that live in the oral cavity. Alongside with the pH 65 and saliva, these devices are targets of biofilm formation especially produced by 66 Candida spp. (1). 67 A combination of factors contribute to Candida sp biofilm formation, salivary 68 flow, low pH, poor oral hygiene and the type of orthodontic material contribute to 69 biofilm colonization and formation (2). During colonization and biofilm formation, oral 70 microbiota secrete enzymes and exopolysaccharides to colonize a surface, thus the 71 biofilm constitutes as a film of organic components that are absorbed from saliva 72 forming an extracellular polymeric matrix and thus the multicellular community 73 (bacteria or fungus) is incorporated into the extracellular matrix (ECM) (1-3). 74The formation of biofilm in orthodontic materials raises concern as, when 75 installed, increases the risk of infection...

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Section: Discussionsupporting

confidence: 88%

Highly Efficient Antibiofilm and Antifungal Activity of Green Propolis againstCandidaspecies in dentistry material

Bezerra

Borges

Alves

et al. 2020

Preprint

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“…Propolis is a resin that presents a number of medicinal properties [8], including anti-inflammatory activity, which could potentiate a possible antifungal topical action. Furthermore, the capacity of propolis to inhibit the biofilm formation of C. albicans has already been reported, along with the low cytotoxicity in human cells with the same PE used in the present study [15,26]. However, the antifungal property of PE has not yet been proved in biofilms formed by Fusarium species.…”

Section: Discussionmentioning

confidence: 55%

“…A possible limitation of this study was the absence of a comparison between the antibiofilm effects of propolis and conventional antifungals. However, it should be pointed out that other works carried out by our group [15,26] have shown propolis to be as or more efficient than other antifungal drugs. Moreover, Fusarium spp.…”

Section: Discussionmentioning

confidence: 84%

Antibiofilm Activity of Propolis Extract onFusariumSpecies from Onychomycosis

et al. 2017

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“…Tobaldini-Valerio et al [28] used the propolis extract against Candida species in both planktonic cells and biofilms, with the authors attributing the observed inhibitory activity to the flavonoids present in the extract. Phenolic compounds impair the growth and formation of C. albicans biofilms, possibly by suppressing genes responsible for adhesion and morphogenesis [29].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Antifungal Activity of the Licania Rigida Leaf Ethanolic Extract against Biofilms Formed by Candida Sp. Isolates in Acrylic Resin Discs

et al. 2019

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Candida sp. treatment has become a challenge due to the formation of biofilms which favor resistance to conventional antifungals, making the search for new compounds necessary. The objective of this study was to identify the composition of the Licania rigida Benth. leaf ethanolic extract and to verify its antifungal activity against Candida sp. and its biofilms. The composition identification was performed using the ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS) technique. The antifungal activity of extract and fluconazole against planktonic cells and biofilms was verified through the minimum inhibitory concentration (MIC) following biofilm induction and quantification in acrylic resin discs by reducing tetrazolic salt, with all isolates forming biofilms within 48 h. Six constituents were identified in the extract, and the compounds identified are derivatives from phenolic compounds such as flavonoids (epi) gallocatechin Dimer, epigallocatechin and gallocatechin, Myricetin-O-hexoside, Myricitrin, and Quercetin-O-rhamnoside. The extract reduced biofilm formation in some of the strains analyzed, namely C. tropicalis URM5732, C. krusei INCQS40042, and C. krusei URM6352. This reduction was also observed in the treatment with fluconazole with some of the analyzed strains. The extract showed significant antifungal and anti-biofilm activities with some of the strains tested.

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Propolis: A Potential Natural Product to Fight Candida Species Infections

Abstract: Propolis is an inhibitor of Candida virulence factors and represents an innovative alternative to fight candidiasis.

Cited by 56 publications

References 51 publications

Highly Efficient Antibiofilm and Antifungal Activity of Green Propolis againstCandidaspecies in dentistry material

Highly Efficient Antibiofilm and Antifungal Activity of Green Propolis againstCandidaspecies in dentistry material

Antibiofilm Activity of Propolis Extract onFusariumSpecies from Onychomycosis

Evaluation of the Antifungal Activity of the Licania Rigida Leaf Ethanolic Extract against Biofilms Formed by Candida Sp. Isolates in Acrylic Resin Discs

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