Propofol suppresses proliferation, invasion, and migration of human melanoma cells via regulating microRNA‐137 and fibroblast growth factor 9

Yu, Hong; Ma, Meina; Zhou, Zhansong; Li, Rui; Guo, Qingduo

doi:10.1002/jcp.28896

Cited by 10 publications

(4 citation statements)

References 32 publications

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“…Relevant research studies have already shown that the antitumor influence of propofol perhaps is closely in connection with miRNA it regulates. For example, propofol can inhibit the proliferation of mammary cancer MCF-7 cells by downregulating the expression of miR-21 [ 20 ] and can also inhibit the activity of melanoma cells by adjusting miR-137 and FGF9 [ 21 ]. miRNAs play important roles in development, cell differentiation, hematopoietic function, cell apoptosis, growth, and immune system.…”

Section: Discussionmentioning

confidence: 99%

Propofol Inhibits Thyroid Cancer Cell Proliferation, Migration, and Invasion by Suppressing SHH and PI3K/AKT Signaling Pathways via the miR-141-3p/BRD4 Axis

Zhang¹,

Tan

Zhang

et al. 2021

Journal of Healthcare Engineering

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Objective. This study explores the effect and mechanism of propofol for thyroid tumor. Methods. Culture human normal thyroid cells Nthy-ori 3-1 and thyroid cancer cell line TPC-1. TPC-1 cells were divided into the propofol group (treated with propofol), miR-141-3p group (transfected with the miR-141-3p mimic), negative control group (transfected with miR-NC), miR-141-3p + pcDNA-BRD4 group (transfected with the miR-141-3p mimic and pcDNA-BRD4), miR-141-3p + pcDNA group (transfected with the miR-141-3p mimic and pcDNA), siBRD4 group (transfected with siBRD4), and si-control group (transfected with si-control). The detection of miR-141-3p and BRD4 expression in cells was done by RT-qPCR, and the dual-luciferase reporter gene method and western blotting were used to verify the targeting relationship between miR-141-3p and BRD4. MTT method was used to test cell proliferation, transwell method was used to test cell migration and invasion, and western blotting was used to test SHH, GLI1, p-PI3K, and p-AKT protein expression. Results. Compared with Nthy-ori 3-1 cells, the expression of miR-141-3p in TPC-1 cells was markedly decreased. Propofol treatment and excessive expression of miR-141-3p could influence the phenotype of TPC-1 cells. BRD4 is one of the target genes of miR-141-3p, and its expression is negatively regulated by miR-141-3p. Overexpression of BRD4 can partially reverse the restraining effect of miR-141-3p on the TPC-1 cell phenotype. Both miR-141-3p and BRD4 can regulate the activity of SHH and PI3K/AKT signaling pathways. Conclusion. Propofol can inhibit the activity of SHH and PI3K/AKT pathways by targeting downregulating BRD4 through miR-141-3p, thereby inhibiting the phenotype of TPC-1 cells.

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Section: Discussionmentioning

confidence: 99%

Propofol Inhibits Thyroid Cancer Cell Proliferation, Migration, and Invasion by Suppressing SHH and PI3K/AKT Signaling Pathways via the miR-141-3p/BRD4 Axis

Zhang¹,

Tan

Zhang

et al. 2021

Journal of Healthcare Engineering

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“…Propofol suppressed migration through regulation of several pathways like phosphoinositide 3‐kinase /protein kinase B pathway [91], miR‐206/Rho‐associated protein kinase 1 axis [92], extracellular‐signal‐regulated kinase/matrix metalloproteinases signaling [93], signal transducer and activator of transcription 3/lncRNA HOX antisense intergenic RNA axis [94], Raf1/extracellular‐signal‐regulated kinase/2, and Wnt/β‐catenin signaling [95]. Propofol also suppressed cell migration and invasion via regulation of lncRNA antisense RNA in the INK4 locus [96], a prostate transmembrane protein, androgen‐induced 1, a transcriptional target of androgen receptor [97], matrix metalloproteinase [98,99], nuclear factor kappa light chain enhancer of activated B cells [100], transforming growth factor β‐1 expression [101], growth factor receptor‐bound protein 2‐mediated signaling [102], expression of neuroepithelial cell transforming 1 gene [103], and fibroblast growth factor 9 [104]. Propofol inhibited lipopolysaccharide‐induced HIF1 activation at the translational level and downstream ATP production in macrophage‐differentiated THP‐1 cells [105].…”

Section: Influence Of Propofol and Isoflurane On Varied Mechanisms Of...mentioning

confidence: 99%

Impact of anesthetics on oncogenic signaling network: a review on propofol and isoflurane

Saha

Das

Chatterjee

et al. 2021

Fundamemntal Clinical Pharma

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Propofol as an intravenous anesthetic and isoflurane as an inhalational/volatile anesthetic continue to be an important part of surgical anesthetic interventions worldwide. The impact of these anesthetics on tumor progression, immune modulation, and survival rates of cancer patients has been widely investigated. Although most of the preclinical studies have provided a beneficial effect of propofol over isoflurane or other volatile anesthetics, several investigations have shown contradictory results, which warrant more preclinical and clinical studies. Propofol mostly exhibits antitumor properties, whereas isoflurane being a cost‐effective anesthetic is frequently used. However, isoflurane has been also reported with protumorigenic activity. This review provides an overall perspective on the network of signaling pathways that may modulate several steps of tumor progression from inflammation, immunomodulation, epithelial–mesenchymal transition (EMT) to invasion, metastasis, angiogenesis, and cancer stemness and extracellular vesicles along with chemotherapeutic applications and clinical status of these anesthetics. A clear understanding of the mechanistic viewpoints of these anesthetics may pave the way for more prospective clinical trials with the ultimate goal of obtaining a safe and optimal anesthetic intervention that would prevent cancer recurrence and may influence better postoperative survival.

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“…Propofol is an intravenous sedative-hypnotic drug used to induce and maintain anesthesia due to its rapid onset and offset of action, making it an ideal medication for surgical procedures. Yu et al (2019) investigated the antitumor properties of propofol in preclinical studies and demonstrated it inhibits proliferation, migration, and invasion of human melanoma cells by regulating microRNA-137 and fibroblast growth factor 9 (FGF9). Zhang et al (2020) uncovered that propofol inhibits invasion and promotes apoptosis of colon cancer cells by preventing the interaction between signal transducer and activator of transcription 3 (STAT3) and the HOX anti-sense intergenic RNA (HOTAIR) promoter, suppressing the Wnt pathway via WIF1.…”

Section: Introductionmentioning

confidence: 99%

Optimization of a tri-drug treatment against lung cancer using orthogonal design in preclinical studies

Tan

Wang

Song

et al. 2023

PeerJ

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A growing body of evidence suggests that anesthetics impact the outcome of patients with cancer after surgical intervention. However, the optimal dose and underlying mechanisms of co-administered anesthetics in lung tumor therapy have been poorly studied. Here, we aimed to investigate the role of combined anesthetics propofol, sufentanil, and rocuronium in treating lung cancer using an orthogonal experimental design and to explore the optimal combination of anesthetics. First, we evaluated the effects of the three anesthetics on the proliferation and invasion of A-549 cells using Cell Counting Kit 8 and Transwell migration and invasion assays. Subsequently, we applied the orthogonal experimental design (OED) method to screen the appropriate concentrations of the combined anesthetics with the most effective antitumor activity. We found that all three agents inhibited the proliferation of A-549 cells in a dose- and time-dependent manner when applied individually or in combination, with the highest differences in the magnitude of inhibition occurring 24 h after combined drug exposure. The optimal combination of the three anesthetics that achieved the strongest reduction in cell viability was 1.4 µmol/L propofol, 2 nmol/L sufentanil, and 7.83 µmol/L rocuronium. This optimal 3-drug combination produced a more beneficial result at 24 h than either single drug. Our results provide a theoretical basis for improving the efficacy of lung tumor treatment and optimizing anesthetic strategies.

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Propofol suppresses proliferation, invasion, and migration of human melanoma cells via regulating microRNA‐137 and fibroblast growth factor 9

Cited by 10 publications

References 32 publications

Propofol Inhibits Thyroid Cancer Cell Proliferation, Migration, and Invasion by Suppressing SHH and PI3K/AKT Signaling Pathways via the miR-141-3p/BRD4 Axis

Propofol Inhibits Thyroid Cancer Cell Proliferation, Migration, and Invasion by Suppressing SHH and PI3K/AKT Signaling Pathways via the miR-141-3p/BRD4 Axis

Impact of anesthetics on oncogenic signaling network: a review on propofol and isoflurane

Optimization of a tri-drug treatment against lung cancer using orthogonal design in preclinical studies

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