Propofol Pretreatment Reduces Ceramide Production and Attenuates Intestinal Mucosal Apoptosis Induced by Intestinal Ischemia/Reperfusion in Rats

Liu, Ke-Xuan; Chen, Shuqing; Huang, Wenqi; Li, Yunsheng; Irwin, Michael G.; Xia, Zhengyuan

doi:10.1213/ane.0b013e3181884bbf

Cited by 53 publications

(41 citation statements)

References 33 publications

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“…It has recently focused greater attention due to its anti-apoptosis capability and antioxidant property [35][36][37][38]. However, the direct effect of propofol on hypoxic osteoblasts has not been reported so far.…”

Section: Discussionmentioning

confidence: 99%

Effect of Propofol Preconditioning on Hypoxic-Cultured Human Osteoblast

Yoon

Shin

Park

et al. 2014

J Korean Dent Soc Anesthesiol

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Background: Angiogenesis has been recognized an essential precondition for osteogenesis. Because reduction and disruption of the blood supply to tissue cause tissue hypoxia, pathological bone loss affected by hypoxia often can occur in various clinical conditions. The effects of propofol on the process of osteogenesis have received little direct attention. Therefore, we investigated the effect of propofol on the growth and function of osteoblasts under hypoxic condition. Methods: After propofol (3, 30, 300 μM) preconditioning for 2 hours, hFOB 1.19 human osteoblast cells were cultured under 1 % oxygen tension for 48 hours. Using real time PCR and western blot analysis, we analyzed the expression of, BMP-2, TGF-β1, type I collagen, osteocalcin, HIF-1s and Akt. Cell viability was also determined by MTT assay. Resul ts: Propofol preconditioning on hypoxic-cultured osteoblast promoted the expressions of BMP-2, TGF-β1, type I collagen and osteocalcin and induced hypoxia-mediated HIF-1 activation and the expression of Akt protein. Propofol with 300 μM significant decreased cell viability compared to control. Concl usi ons: Clinically relevant concentrations of propofol are not cytotoxic to hypoxic osteoblasts in vitro. Propofol preconditioning on hypoxic-cultured osteoblast stimulates proliferation and differentiation of osteoblast through induced expression of BMP-2, TGF-β1, type I collagen and osteocalcin. Propofol might promote angiogenesis and bone regeneration under hypoxic condition.

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Section: Discussionmentioning

confidence: 99%

Effect of Propofol Preconditioning on Hypoxic-Cultured Human Osteoblast

Yoon

Shin

Park

et al. 2014

J Korean Dent Soc Anesthesiol

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“…Several studies have reported that propofol limits myocardial ischemia/reperfusion injury with an associated reduction in apoptotic cell deaths in rats (13), and it attenuates intestinal ischemia/reperfusion induced intestinal epithelial apoptosis which might be attributable to its antioxidant property modulating the ceramide pathway (14). Moreover, propofol protects hepatic L02 cells from These results suggest that the application of propofol has potential to reduce apoptosis through the JNK pathway and Bim down-regulation.…”

Section: Discussionmentioning

confidence: 74%

Propofol Reduces Hydrogen Peroxide-Induced Apoptosis through Down-Regulating Bim Expression in Alveolar Epithelial Cells

et al. 2013

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Propofol is an intravenous anesthetic agent widely used to introduce and maintain anesthesia during surgical procedures, and it has often been used in recent years as a sedative in patients with acute lung injury (ALI). A common cause of ALI is sepsis, and apoptosis in vascular endothelium and alveolar epithelial cells (AECs) during sepsis plays an important role in the pathologic formation of ALI. Propofol has drawn greater attention due to its anti-inflammatory effect and anti-apoptosis capability. It was already found that propofol inhibited MCP-1 mRNA expression and secretion, as well as the phosphorylation of

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“…Prophylactic antiapoptotic treatment can be an effective therapeutic strategy for the prevention of I/R injury [22] . Thiopental is a highly lipid-soluble anesthetic, which has indicated antioxidant effect by inhibiting lipid peroxidation in ischemia/reperfusion injury [17] .…”

Section: Discussionmentioning

confidence: 99%

Ratlarda Böbrek İşemi/Reperfüzyon Hasarına Karşı Tiyopentalin Koruyucu Etkisi

Çoşkuner¹,

Türkay²,

Yıldız³

et al. 2016

Kafkas Univ Vet Fak Derg

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The aim of this study was to determine optimal protective thiopental dose in renal ischemia/reperfusion (I/R) damage. This study was carried out on 42 rats. After abdominal midline incision, left renal artery was occluded. After 60 min of ischemia and reperfusion, a left nephrectomy was performed. Surgical intervention was made for the six rat groups (excluding sham group). In control group, 60 minutes of ischemia and reperfusion were applied to left kidneys. Thiopental (3, 10, 30, 60, 80 mg/ kg) was applied intra-peritoneally to the rats except sham and control groups 15 minutes before reperfusion. After reperfusion, IL-1α, IL-6, TNF-α levels; SOD activity; MDA and NO levels were measured. There was no decrease in IL-6 or TNF-α levels in groups at all doses. IL-1α levels only decreased in the group receiving 3 mg/kg thiopental. Only the group receiving 80 mg/kg observed decrease in MDA levels. The rats receiving 30 mg/kg showed significant increase in SOD levels. The rats receiving 3 mg/ kg detected significant increase in glutathione peroxidase (GPx) levels. The groups receiving 10, 30, and 60 mg/kg thiopental showed decrease in nitric oxide (NO) levels. According to study results, different doses of thiopental affected each biochemical indicator in renal I/R damage. Keywords: Renal Ischemia/Reperfusion, Thiopental, Rat Ratlarda Böbrek İşemi/Reperfüzyon Hasarına Karşı Tiyopentalin Koruyucu Etkisi ÖzetÇalışmada ratlarda farklı dozlarda uygulanan tiyopentalin renal I/R hasarına karşı koruyucu optimal dozunun saptanması amaçlandı. Bu çalışma 42 ratta yapıldı. Abdominal orta hat insizyonu sonrası sol renal arter okluzyonu yapıldı. Altmış dakika işemi ve reperfüzyondan sonra sol nefrektomi yapıldı. Altışar rattan oluşan gruplarda (sham grup dışında) cerrahi prosedür uygulandı. Kontrol grubunda sol böbreklere 60 dk işemi ve reperfüzyon uygulandı. Sham ve kontrol grubu dışındaki ratlara reperfüzyondan 15 dk önce tiyopental (3, 10, 30, 60, 80 mg/kg) intra-peritoneal olarak uygulandı. Reperfüzyon sonunda IL-1α, IL-6, TNF-α seviyeleri; SOD aktivitesi; MDA ve NO düzeyleri ölçüldü. Gruplarda IL-6 ve TNF-α seviyelerinde azalma olmadı. Yalnızca 3 mg/kg tiyopental uygulanan grupta IL-1α seviyesi azaldı. MDA düzeylerinde düşme yalnızca 80 mg/kg tiyopental uygulanan grupta görüldü. 30 mg/ kg tiyopental uygulanan ratlarda SOD düzeylerinde anlamlı yükseklik saptandı. 3 mg/kg tiyopental uygulanan ratlarda glutatyon peroksidaz (GPx) düzeylerinde anlamlı yükseklik saptandı. Nitrik oksit seviyesindeki düşme 10, 30 ve 60 mg/kg tiyopental uygulanan gruplarda görüldü. Çalışma sonuçlarına göre, renal işemi reperfüzyon hasarında her bir biyokimyasal belirteci tiyopentalin farklı dozları etkiledi.

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Propofol Pretreatment Reduces Ceramide Production and Attenuates Intestinal Mucosal Apoptosis Induced by Intestinal Ischemia/Reperfusion in Rats

Abstract: The findings indicate that propofol pretreatment attenuates II/R-induced intestinal epithelial apoptosis, which might be attributable to its antioxidant property modulating the ceramide pathway.

Cited by 53 publications

References 33 publications

Effect of Propofol Preconditioning on Hypoxic-Cultured Human Osteoblast

Effect of Propofol Preconditioning on Hypoxic-Cultured Human Osteoblast

Propofol Reduces Hydrogen Peroxide-Induced Apoptosis through Down-Regulating Bim Expression in Alveolar Epithelial Cells

Ratlarda Böbrek İşemi/Reperfüzyon Hasarına Karşı Tiyopentalin Koruyucu Etkisi

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