“…When recordings were performed from the mPFC or basolateral amygdala (BLA), coronal brain slices (300 m thick) were prepared using a Vibratome 3000 (Vibratome, St. Louis, MO) in artificial CSF [ACSF; containing the following (in mM): 125 NaCl, 4.4 KCl, 1.5 MgSO 4 , 1.0 NaH 2 PO 4 , 26 NaHCO 3 , 10 glucose, 2.5 CaCl 2 , pH 7.4, 290 -300 mOsm/l]. When recordings were performed from the CA1 field, the hippocampus was isolated from the brain and transverse slices (400 m thick) were prepared as described previously (Nakagawa et al, 1999;Takamatsu et al, 2005) using a Vibratome 3000 in a sucrose-based cutting solution [containing the following (in mM): 234 sucrose, 25 KCl, 1.25 NaH 2 PO 4 , 10 MgSO 4 , 26 NaHCO 3 , 11 glucose, 0.5 CaCl 2 ]. The slices were maintained at room temperature in ACSF continuously bubbled with 95% O 2 /5% CO 2 .…”