Propofol exhibits a tumor‑suppressive effect and regulates cell viability, migration and invasion in bladder carcinoma by targeting the microRNA‑10b/HOXD10 signaling pathway

Qi, Zongcai; Yuan, Lei; Sun, Nenghong

doi:10.3892/ol.2019.10968

Cited by 10 publications

(10 citation statements)

References 35 publications

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“…Garib et al (25) reported that propofol increased the migration of breast cancer cells; however, Miao et al (26) suggested that propofol inhibited the invasion of colon cancer cells. Qi et al (27) revealed a tumor-suppressive effect of propofol in BC by suppressing cell proliferation, migration and invasion. In the present study, it was revealed that propofol inhibited the viability, migration and invasion of BC cells in vitro, and suppressed tumor xenograft growth in vivo; this was consistent with previous reports that propofol exhibits antitumor effects.…”

Section: Discussionmentioning

confidence: 99%

Propofol modulates the proliferation, invasion and migration of bladder cancer cells through the miR‑145‑5p/TOP2A axis

Zhang

et al. 2021

Mol Med Rep

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Propofol-based anesthesia has been reported to reduce the recurrence and metastasis of a number of cancer types following surgical resection. However, the effects of propofol in bladder cancer (BC) are yet to be fully elucidated. The aim of the present study was to investigate the functions of propofol in BC and their underlying mechanisms. In the study, the expression of microRNA (miR)-145-5p in BC tissues and cell lines was evaluated using reverse transcription-quantitative PCR, and the effects of propofol on BC cells were determined using cell viability, wound healing and Transwell cell invasion assays, bioinformatics analysis, western blotting, immunohistochemistry and in vivo tumor xenograft models. It was found that propofol significantly suppressed the proliferation, migration and invasion of BC cells in vitro. In addition, propofol induced miR-145-5p expression in a time-dependent manner, and miR-145-5p knockdown attenuated the inhibitory effects of propofol on the proliferation, migration and invasion of BC cells. Topoisomerase II α (TOP2A) was a direct target of miR-145-5p, and silencing TOP2A reversed the effects of miR-145-5p knockdown in propofol-treated cells. Furthermore, propofol suppressed tumor xenograft growth, which was partially attenuated by miR-145-5p knockdown. The present study provided novel insight into the advantages of surgical intervention with propofol anesthesia in patients with BC.

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Section: Discussionmentioning

confidence: 99%

Propofol modulates the proliferation, invasion and migration of bladder cancer cells through the miR‑145‑5p/TOP2A axis

Zhang

et al. 2021

Mol Med Rep

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“…20 In addition, propofol plays an antitumor role through regulating the viability, migration and invasion of bladder cancer by targeting the microRNA-10b/HOXD10 signaling pathway. 21 On the other hand, studies have confirmed that midazolam and Rop synergistically inhibit bone cancer pain in rats with different mechanisms. 22 Moreover, Rop also plays an important role in tumors.…”

Section: Discussionmentioning

confidence: 99%

<p>Ropivacaine Inhibits the Growth, Migration and Invasion of Gastric Cancer Through Attenuation of WEE1 and PI3K/AKT Signaling via miR-520a-3p</p>

Zhang¹,

Xing²,

Gu³

et al. 2020

OTT

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Background: Metastasis remains one of the greatest challenges involved in treating gastric cancer (GC). Ropivacaine (Rop) is not only a well-documented local anesthetic medicament but also has been reported to exert an antitumor role in cancer development. This study explored the effects of ropivacaine on the growth, migration and invasion of gastric cancer and the underlying mechanisms. Methods: Cell Counting Kit-8 (CCK8) assay was conducted to test the effect of Rop on the proliferation of AGS and BGC-823 GC cells. Moreover, cell apoptosis, migration and invasion were examined by flow cytometry and transwell assay, respectively. The expression of miR-520a-3p was determined by qRT-PCR. miRNA targeting sites were analyzed using bioinformatics analysis and dual-luciferase reporter assay. Protein levels of WEE1 and PI3K/ AKT were detected by Western blot. Furthermore, the tumor-forming experiment of nude mice was used to detect the growth of cells in vivo. Results: Rop inhibited proliferation but promoted apoptosis of GC cells. Besides, the migration and invasion of GC cells were also inhibited by Rop. Moreover, miR-520a-3p expression was enhanced by Rop, and transfection with miR-520a-3p mimic decreased cell proliferation, migration and invasion. The upregulation of miR-520a-3p was partly contributed to the inhibitory effect of ropivacaine on GC cell lines. Finally, Rop inactivated WEE1 and PI3K/AKT pathway via upregulation of miR-520a-3p. Conclusion: Our results suggested that Rop decreased growth, migration and invasion of GC cells via regulating miR-520a-3p expression and further inactivated WEE1 and PI3K/ AKT signaling pathways.

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“…In addition to anesthesia, propofol also affects immunity, inflammation, and ischemia reperfusion injury 4 , 19 . Propofol has been reported to induce antitumor immunity by promoting T-assisted cell activation and differentiation 20 – 22 and can inhibit the viability, migration, and invasion of bladder cancer T24 cells in vitro 23 . In this study, we explored the effects of propofol on the proliferation, migration, and stem-like properties in three bladder cancer cell lines, identified its underlying mechanisms, and further validated its effects in primary human bladder cancer cells and mice xenografts in vivo .…”

Section: Discussionmentioning

confidence: 99%

Propofol Inhibits the Proliferation, Migration, and Stem-like Properties of Bladder Cancer Mainly by Suppressing the Hedgehog Pathway

Zhang

Guo

et al. 2021

Cell Transplant

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Bladder cancer is one of the most common malignancies. The existence of bladder cancer stem cells (BCSCs) has been suggested to underlie bladder tumor initiation and recurrence. Propofol is a commonly used intravenous anesthetic. Here, we find that propofol can dramatically block the activation of Hedgehog pathway in BCSCs. The propofol strongly repressed the growth of cancer cells. Attenuated proliferation and enhanced apoptosis of tumor cells were observed upon propofol stimulation. Furthermore, propofol reduced the self-renewal ability of BCSCs as well as the tumor formation. In conclusion, propofol is potentially used as a novel therapeutic agent for bladder cancer by targeting self-renewal through inhibiting Hedgehog pathway.

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Propofol exhibits a tumor‑suppressive effect and regulates cell viability, migration and invasion in bladder carcinoma by targeting the microRNA‑10b/HOXD10 signaling pathway

Cited by 10 publications

References 35 publications

Propofol modulates the proliferation, invasion and migration of bladder cancer cells through the miR‑145‑5p/TOP2A axis

Propofol modulates the proliferation, invasion and migration of bladder cancer cells through the miR‑145‑5p/TOP2A axis

<p>Ropivacaine Inhibits the Growth, Migration and Invasion of Gastric Cancer Through Attenuation of WEE1 and PI3K/AKT Signaling via miR-520a-3p</p>

Propofol Inhibits the Proliferation, Migration, and Stem-like Properties of Bladder Cancer Mainly by Suppressing the Hedgehog Pathway

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