Propionate inhibits glucose‐induced insulin secretion in isolated rat pancreatic islets

Ximenes, Helena Maria; Hirata, Aparecida Emiko; Rocha, M. S.; Curi, Rui; Carpinelli, Ângelo Rafael

doi:10.1002/cbf.1297

Cited by 47 publications

(35 citation statements)

References 37 publications

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“…Propionate serves as a precursor for gluconeogenesis and reduces the synthesis of hepatic cholesterol (54). Propionate was also associated with a decrease in insulin secretion in pancreatic islet cells of rats (55). Butyric acid serves as the main energy source for colonocytes and protects against inflammation (56,57).…”

Section: Discussionmentioning

confidence: 99%

Effect of Whole-Grain Barley on the Human Fecal Microbiota and Metabolome

Angelis

Montemurno

Vannini

et al. 2015

Appl Environ Microbiol

132

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In this study, we compared the fecal microbiota and metabolomes of 26 healthy subjects before (HS) and after (HSB) 2 months of diet intervention based on the administration of durum wheat flour and whole-grain barley pasta containing the minimum recommended daily intake (3 g) of barley ␤-glucans. Metabolically active bacteria were analyzed through pyrosequencing of the 16S rRNA gene and community-level catabolic profiles. Pyrosequencing data showed that levels of Clostridiaceae (Clostridium orbiscindens and Clostridium sp.), Roseburia hominis, and Ruminococcus sp. increased, while levels of other Firmicutes and Fusobacteria decreased, from the HSB samples to the HS fecal samples. Community-level catabolic profiles were lower in HSB samples. Compared to the results for HS samples, cultivable lactobacilli increased in HSB fecal samples, while the numbers of Enterobacteriaceae, total coliforms, and Bacteroides, Porphyromonas, Prevotella, Pseudomonas, Alcaligenes, and Aeromonas bacteria decreased. Metabolome analyses were performed using an amino acid analyzer and gas chromatography-mass spectrometry solidphase microextraction. A marked increase in short-chain fatty acids (SCFA), such as 2-methyl-propanoic, acetic, butyric, and propionic acids, was found in HSB samples with respect to the HS fecal samples. Durum wheat flour and whole-grain barley pasta containing 3% barley ␤-glucans appeared to be effective in modulating the composition and metabolic pathways of the intestinal microbiota, leading to an increased level of SCFA in the HSB samples.

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Section: Discussionmentioning

confidence: 99%

Effect of Whole-Grain Barley on the Human Fecal Microbiota and Metabolome

Angelis

Montemurno

Vannini

et al. 2015

Appl Environ Microbiol

132

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“…However, it is not clear why butyrate does not inhibit GSIS in our study as FFAR2 signaling has been reported to also inhibit GSIS, as reported by Tang. 14 Taken together, our data demonstrates that propionate inhibits GSIS, an observation consistent with previously published findings. 11 Future studies need to consider species difference in ligand preference especially when exploring the role of FFAR3 in human versus mouse islets.…”

Section: Discussionmentioning

confidence: 99%

“…10 Compared to acetate, even fewer studies have examined propionate and butyrate in GSIS. One study observed that propionate inhibits GSIS 11 and another study observed that butyrate augments GSIS. 12 As these receptors are expressed in b cells and GPCRs have a well-described role in insulin secretion, 13 investigating if SCFAs mediate GSIS through their cognate receptors is needed.…”

Section: Introductionmentioning

confidence: 99%

FFAR3 modulates insulin secretion and global gene expression in mouse islets

Priyadarshini¹,

Layden

2015

Islets

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The short chain fatty acid (SCFA) receptor (free fatty acid receptor-3; FFAR3) is expressed in pancreatic b cells; however, its role in insulin secretion is not clearly defined. Here, we examined the role of FFAR3 in insulin secretion. Using islets from global knockout FFAR3 (Ffar3 ¡/¡ ) mice, we explored the role of FFAR3 and ligand-induced FFAR3 signaling on glucose stimulated insulin secretion. RNA sequencing was also performed to gain greater insight into the impact of FFAR3 deletion on the islet transcriptome. First exploring insulin secretion, it was determined that Ffar3 ¡/¡ islets secrete more insulin in a glucose-dependent manner as compared to wildtype (WT) islets. Next, exploring its primary endogenous ligand, propionate, and a specific agonist for FFAR3, signaling by FFAR3 inhibited glucosedependent insulin secretion, which occurred through a Ga i/o pathway. To help understand these results, transcriptome analyses by RNA-sequencing of Ffar3 ¡/¡ and WT islets observed multiple genes with well-known roles in islet biology to be altered by genetic knockout of FFAR3. Our data shows that FFAR3 signaling mediates glucose stimulated insulin secretion through Ga i/o sensitive pathway. Future studies are needed to more rigorously define the role of FFAR3 by in vivo approaches.

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“…Fermentation with SOY demonstrated significantly higher propionate production than other pectin samples (HMP and SBP) and FOS during 30 hrs (P < 0.05). Propionate has been shown to lower glucose-induced insulin secretion in isolated pancreatic islet cells of rats [35]. It is also reported that propionate has anti-proliferative effect towards colon cancer [36] [37] and is related to weight control and feeding behavior [38] [39].…”

Section: Short-chain Fatty Acid Analysismentioning

confidence: 96%

Fermentation Patterns of Various Pectin Sources by Human Fecal Microbiota

Min¹,

Koo²,

Park³

et al. 2015

FNS

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High Methoxy Pectin (HMP), Sugar Beet Pectin (SBP), Soy Pectin (SOY), and Fructooligosaccharide (FOS, as a positive control) were used to determine fermentation properties considering applicability as functional foods, particularly related to colon health. Certain beneficial effects of carbohydrates in humans can be postulated as being due to microorganisms and metabolites (short-chain fatty acids (SCFAs)). Fecal samples were collected and incubated anaerobically with HMP, SBP, SOY, and FOS at 37˚C. The average degree of polymerization (DP) of HMP, SBP, and SOY was 492, 3729, and 1510, respectively. Degree of pectin methylation of each sample was 76.0% (HMP), 21.2% (SBP), and 22.8% (SOY). Total SCFAs in SOY showed the highest value compared to other samples, especially having the highest concentration of propionic acid (P < 0.05). While fermentation with FOS showed higher butyrate production, the total SCFAs with SOY, HMP, and SBP were significantly higher than FOS over 30 h (P < 0.05). From the denaturing gradient gel electrophoresis (DGGE) analysis, changes of microbiota composition were found. In conclusion, pectin samples, especially soy pectin, stimulated production of total SCFAs and composition of human fecal microbiota was modulated. Therefore, pectin samples may alter the composition of fecal microbiota and improve the colonic health.

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Propionate inhibits glucose‐induced insulin secretion in isolated rat pancreatic islets

Cited by 47 publications

References 37 publications

Effect of Whole-Grain Barley on the Human Fecal Microbiota and Metabolome

Effect of Whole-Grain Barley on the Human Fecal Microbiota and Metabolome

FFAR3 modulates insulin secretion and global gene expression in mouse islets

Fermentation Patterns of Various Pectin Sources by Human Fecal Microbiota

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