Propineb induced notochord deformity, craniofacial malformation, and osteoporosis in zebrafish through dysregulated reactive oxygen species generation

Yuan, Wei; Hu, Ying; Lu, Chen; Zhang, J S; Liu, Yanyong; Li, X.; Jia, Kun; Huang, Yong; Li, Zekun; Chen, Xiaomei; Wang, F.; Yi, Xiaolan; Che, Xiaofang; Xiong, Haibin; Cheng, Bo; Ma, Jinze; Zhao, Yan; Lu, Huiqiang

doi:10.1016/j.aquatox.2023.106596

Cited by 2 publications

(1 citation statement)

References 41 publications

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“…Alizarin Red staining, commonly used to visualize the degree of osteogenic differentiation [ 33 , 44 ], indicated a significant increase in new bone deposition in both Zinc scaffold and Zinc scaffold/BF Exo groups compared to the Kirschner wire group, as evidenced by intense red coloration. Calcein Green fluorochrome labeling was used to assess the toxicity of the materials and indicated a higher number of viable cells in both experimental groups [ 45 , 46 ]. Additionally, Methylene Blue-Acid Magenta staining displayed clear visualization of osteoblasts, extracellular matrix, and osteoid, aiding in the characterization of static bone formation parameters [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

3D-printed porous zinc scaffold combined with bioactive serum exosomes promotes bone defect repair in rabbit radius

Zhang,

Pei,

et al. 2024

Aging

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Currently, the repair of large bone defects still faces numerous challenges, with the most crucial being the lack of large bone grafts with good osteogenic properties. In this study, a novel bone repair implant (degradable porous zinc scaffold/BF Exo composite implant) was developed by utilizing laser melting rapid prototyping 3D printing technology to fabricate a porous zinc scaffold, combining it under vacuum conditions with highly bioactive serum exosomes (BF EXO) and Poloxamer 407 thermosensitive hydrogel. The electron microscope revealed the presence of tea saucer-shaped exosomes with a double-layered membrane structure, ranging in diameter from 30-150 nm, with an average size of 86.3 nm and a concentration of 3.28E+09 particles/mL. In vitro experiments demonstrated that the zinc scaffold displayed no significant cytotoxicity, and loading exosomes enhanced the zinc scaffold's ability to promote osteogenic cell activity while inhibiting osteoclast activity. In vivo experiments on rabbits indicated that the hepatic and renal toxicity of the zinc scaffold decreased over time, and the loading of exosomes alleviated the hepatic and renal toxic effects of the zinc scaffold. Throughout various stages of repairing radial bone defects in rabbits, loading exosomes reinforced the zinc scaffold's capacity to enhance osteogenic cell activity, suppress osteoclast activity, and promote angiogenesis. This effect may be attributed to BF Exo's regulation of p38/STAT1 signaling. This study signifies that the combined treatment of degradable porous zinc scaffolds and BF Exo is an effective and biocompatible strategy for bone defect repair therapy.

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Section: Discussionmentioning

confidence: 99%

3D-printed porous zinc scaffold combined with bioactive serum exosomes promotes bone defect repair in rabbit radius

Zhang,

Pei,

et al. 2024

Aging

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Early stress exposure on zebrafish development: effects on survival, malformations and molecular alterations

Valcarce,

Sellés-Egea,

Riesco

et al. 2024

Fish Physiol Biochem

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The effects of stress during early vertebrate development can be especially harmful. Avoiding stressors in fish larvae is essential to ensure the health of adult fish and their reproductive performance and overall production. We examined the consequences of direct exposure to successive acute stressors during early development, including their effects on miR-29a and its targets, survival, hatching and malformation rates, larval behaviour and cartilage and eye development. Our aim was to shed light on the pleiotropic effects of early-induced stress in this vertebrate model species. Our results showed that direct exposure to successive acute stressors during early development significantly upregulated miR-29a and downregulated essential collagen transcripts col2a1a, col6a2 and col11a1a, decreased survival and increased malformation rates (swim bladder, otoliths, cardiac oedema and ocular malformations), promoting higher rates of immobility in larvae. Our results revealed that stress in early stages can induce different eye tissular architecture and cranioencephalic cartilage development alterations. Our research contributes to the understanding of the impact of stressful conditions during the early stages of zebrafish development, serving as a valuable model for vertebrate research. This holds paramount significance in the fields of developmental biology and aquaculture and also highlights miR-29a as a potential molecular marker for assessing novel larval rearing programmes in teleost species.

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Propineb induced notochord deformity, craniofacial malformation, and osteoporosis in zebrafish through dysregulated reactive oxygen species generation

Cited by 2 publications

References 41 publications

3D-printed porous zinc scaffold combined with bioactive serum exosomes promotes bone defect repair in rabbit radius

3D-printed porous zinc scaffold combined with bioactive serum exosomes promotes bone defect repair in rabbit radius

Early stress exposure on zebrafish development: effects on survival, malformations and molecular alterations

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