Propidium iodide as a nuclear marker in immunofluorescence. II. Use with cellular identification and viability studies

Yeh, Chang-Jing G.; Hsi, Bae‐Li; Faulk, W. Pagé

doi:10.1016/0022-1759(81)90174-5

Cited by 184 publications

(111 citation statements)

References 8 publications

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“…Cell viability was determined with propidium iodide (PI; Ex max ϭ 540 nm, Em max ϭ 625 nm; Sigma), which stains dead cells only (37). PI was used at 5 g/ml in a cell suspension adjusted to 10 6 cells/ml.…”

Section: Flow Cytometric Determination Of Cell Viability With Propidimentioning

confidence: 99%

7‐Ketocholesterol favors lipid accumulation and colocalizes with Nile Red positive cytoplasmic structures formed during 7‐ketocholesterol–induced apoptosis: Analysis by flow cytometry, FRET biphoton spectral imaging microscopy, and subcellular fractionation

et al. 2005

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Background: Oxidized low-density lipoproteins play key roles in atherosclerosis. Their toxicity is at least in part due to 7-ketocholesterol (7KC), which is a potent inducer of apoptosis. In this study on human promonocytic U937 cells, we determined the effects and the interactions of 7KC with cellular lipids during 7KC-induced apoptosis. Methods: Morphologic and functional changes were investigated by microscopic and flow cytometric methods after staining with propidium iodide, 3,3Ј-dihexyloxacarbocyanine iodide, and Hoechst 33342. Cellular lipid content was identified by using filipin to quantify free cholesterol and Nile Red (NR), which emit a yellow or orangered fluorescence in the presence of neutral and polar lipids, respectively. After staining with NR, interactions of 7KC with cellular lipids were identified by fluorescence resonance energy transfer biphoton spectral imaging confocal microscopy and by subcellular fractionation, gas chromatography, and mass spectrometry.

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Section: Flow Cytometric Determination Of Cell Viability With Propidimentioning

confidence: 99%

7‐Ketocholesterol favors lipid accumulation and colocalizes with Nile Red positive cytoplasmic structures formed during 7‐ketocholesterol–induced apoptosis: Analysis by flow cytometry, FRET biphoton spectral imaging microscopy, and subcellular fractionation

et al. 2005

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“…This demonstrates that FL-SLFЈ can be used to label cells that express an intracellular FKBP12(F36V) fusion protein. Flow cytometry can also be used to coherently measure cell viability (with propidium iodide uptake) and cell health (with forward and side scatter) (20,21). FL-SLFЈ was incubated in cells at a concentration as high as 10 M, and for time periods as long as 24 h, with no observable effects on cell viability (determined by uptake of the dye propidium iodide), and no changes in the light scatter properties of the cells (data not shown).…”

Section: A Fluorescent Conjugate Of the Fkbp12(f36v) Ligand Slfmentioning

confidence: 99%

A general approach for chemical labeling and rapid, spatially controlled protein inactivation

Marks

Braun

Nolan

2004

Proc. Natl. Acad. Sci. U.S.A.

103

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“…Propidium iodide (2 &L of a 250-pg/mL solution; Sigma) was added to each sample immediately before acquisition in the flow microfluorimeter to exclude dead cells from the analysis. 23 …”

Section: Cytokine Stimulation and Phenotype Analysis Of Hibecmentioning

confidence: 99%

Hepatic allograft rejection: Regulation of the immunogenicity of human intrahepatic biliary epithelial cells

et al. 1996

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lntrahepatic biliary epithelial cells were immunomagnetically purified from specimens of disaggregated human liver and were propagated in vitro. After three passes in culture, the cells were shown to be over 85% pure with contaminating leukocytes and endothelial cells constituting less than 2% of the population. Sensitive flow microfluorimetric analysis was performed after immunofluorescence labeling to quantify expression of both class I and class II major histocompatibility (MHC) antigens and the adhesion molecules intercellular adhesion molecule-1 (CAM-l), vascular cell adhesion molecule-1 (VCAM-l), and lymphocyte function-associated antigen-3 (LFA-3). It was shown that resting human intrahepatic biliary epithelial cells (HIBEC) expressed class I MHC antigens, ICAM-1, and relatively low levels of LFA-3. Stimuntrahepatic bile ducts constitute a major target in

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Propidium iodide as a nuclear marker in immunofluorescence. II. Use with cellular identification and viability studies

Cited by 184 publications

References 8 publications

7‐Ketocholesterol favors lipid accumulation and colocalizes with Nile Red positive cytoplasmic structures formed during 7‐ketocholesterol–induced apoptosis: Analysis by flow cytometry, FRET biphoton spectral imaging microscopy, and subcellular fractionation

7‐Ketocholesterol favors lipid accumulation and colocalizes with Nile Red positive cytoplasmic structures formed during 7‐ketocholesterol–induced apoptosis: Analysis by flow cytometry, FRET biphoton spectral imaging microscopy, and subcellular fractionation

A general approach for chemical labeling and rapid, spatially controlled protein inactivation

Hepatic allograft rejection: Regulation of the immunogenicity of human intrahepatic biliary epithelial cells

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