Propiconazole inhibits the sterol 14α-demethylase in Glomus irregulare like in phytopathogenic fungi

Calonne, Maryline; Sahraoui, Anissa Lounès-Hadj; Campagnac, Estelle; Debiane, Djouher; Laruelle, Frédéric; Grandmougin-Ferjani, Anne; Fontaine, Joël

doi:10.1016/j.chemosphere.2011.12.027

Cited by 21 publications

(12 citation statements)

References 66 publications

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“…For example, it was shown using in vitro systems that direct contact of the extraradical structures with fungicidal substances such as propiconazole (sterol biosynthesis inhibitor) was necessary to reduce hyphal length (threshold level of 0.02 mg L −1 ) and the spore number (threshold level of 0.2 mg L −1 ) in the hyphal compartment (Table S2). In pot experiments, the results are contradictory, ranging from neutral to negative effects on the activity of external hyphae and hyphal P uptake when propiconazole in its formulated product (Tilt 250EC®) was applied to the soil. Internal fungal structures were not affected in these studies.…”

Section: Exposure Of Amf To Pesticides During Crop Growthmentioning

confidence: 99%

Arbuscular mycorrhizal fungi and their response to pesticides

Hage‐Ahmed

Rosner

Steinkellner

2018

Pest Management Science

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Arbuscular mycorrhizal fungi (AMF) form symbioses with the majority of plant species and can provide multiple benefits to the host plant. In agro‐ecosystems, the abundance and community structure of AMF are affected by agricultural management practices. This review describes and discusses current knowledge on the effects of inorganic and organic chemical pesticides on AMF in the conflicting area between agricultural use and environmental concerns. Variable effects have been reported following chemical pesticide use, ranging from neutral to positive and negative. Moreover, a species‐specific reaction has been documented. The reported effects of pesticides on arbuscular mycorrhizal symbiosis are very diverse, and even when the same substance is investigated, the results are often contradictory. These effects depend on many parameters, such as the active substance, the mode of action, the mode of application and the dosage. In the field, determinants such as the physico‐chemical behavior of the active substances, the soil type and other soil microorganisms contribute to the fate of pesticides and thus the amount of active substances to which AMF are exposed. This review highlights that the fate of AMF following pesticide use needs to be addressed in a broader agro‐ecosystem context. © 2018 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Section: Exposure Of Amf To Pesticides During Crop Growthmentioning

confidence: 99%

Arbuscular mycorrhizal fungi and their response to pesticides

Hage‐Ahmed

Rosner

Steinkellner

2018

Pest Management Science

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“…In vitro cultivation systems offer several advantages, among which, the absence of any confounding effects caused by unwanted contaminants or environmental factors (e.g., soil physico-chemical parameters) and the easy non-destructive observations of growth and development of fungi. Spore germination (Chiocchio et al, 2000;Zocco et al, 2008;Buysens et al, 2015), root colonization (Campagnac et al, 2008(Campagnac et al, , 2009Calonne et al, 2010Calonne et al, , 2012, anastomosis formation (Cardenas-Flores et al, 2011;de Novais et al, 2019a,b), sterol biosynthesis pathway (Campagnac et al, 2010;Calonne et al, 2012) and transport of nutrients (e.g., phosphorus) from fungus to plant (Zocco et al, 2011) have been investigated in vitro in presence of different types of fungicides. Results differed significantly with fungicide and dose of application.…”

Section: Introductionmentioning

confidence: 99%

Fungicides With Contrasting Mode of Action Differentially Affect Hyphal Healing Mechanism in Gigaspora sp. and Rhizophagus irregularis

et al. 2021

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Fungicides are widely used in conventional agriculture to control fungal diseases, but may also affect non-target microorganisms such as arbuscular mycorrhizal (AM) fungi. These root symbionts develop extended mycelial networks within the soil via mechanisms such as anastomosis that indistinctly concerns intact and damaged hyphae, the latter being named hyphal healing mechanism (HHM). The HHM differs between Glomeraceae and Gigasporaceae. However, the effects of fungicides on this mechanism in unknown. Here, the impact of azoxystrobin, pencycuron, flutolanil, and fenpropimorph at 0.02 and 2 mg L–1 were tested in vitro on the HHM of Gigaspora sp. MUCL 52331 and Rhizophagus irregularis MUCL 41833, and repair events visualized carefully under a dissecting bright-field light microscope. Azoxystrobin was the more detrimental for both AM fungi at 2 mg L–1, while fenpropimorph impacted only R. irregularis (stimulating at low and inhibiting at high concentration). Conversely, flutolanil and pencycuron did not impact any of the two AM fungi. The mechanisms involved remains to be elucidated, but perturbation in the still-to-be firmly demonstrated spitzenkörper or in sterols content as well as a process of hormesis are possible avenues that deserve to be explored in view of a rationale management of chemicals to control fungal pathogens without harming the beneficial AM fungi.

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“…These lipids are known to regulate chitin synthetase activities, which mediate chitin synthesis essential for cell wall formation and cell differentiation in micromycetes (Martin and Konopka 2004;Mysyakina and Funtikova 2007;Mysyakina and Feofilova 2011). Growth inhibition of the same AMF was also described when the fungus was treated with fungicides resulting from sterol metabolism inhibition (Campagnac et al 2008(Campagnac et al , 2009Calonne et al 2012). A disturbance in sterol biosynthesis can also affect trophic exchanges and, thus, the functioning of mycorrhizal symbiosis.…”

Section: Control Anthracenementioning

confidence: 88%

“…Arbuscular mycorrhizal fungi (AMF) are ubiquitous soil microorganisms that form symbiotic associations with nearly 80% of plant species (Bonfante and Perotto 1995). These fungi are considered essential for the survival of many plants in natural competitive situations and are important under adverse soil, chemical, and physical conditions such as drought, cold, salinity, and pollution (Augé 2001;Hildebrandt et al 2007;Campagnac et al 2008Campagnac et al , 2010Calonne et al 2012;Porcel et al 2012). The efficiency of this phytotechnology is, nevertheless, dependant on plant and AMF resistance to PAH toxicity.…”

Section: Introductionmentioning

confidence: 99%

Impact of anthracene on the arbuscular mycorrhizal fungus lipid metabolism

Calonne

Fontaine

DebianeD.

et al. 2014

Botany

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Anthracene, a low-molecular-weight polycyclic aromatic hydrocarbon (PAH) originating mainly from anthropogenic activities, represents one of the major persistent organic pollutants frequently detected in polluted soils. A few studies have reported the negative effect of PAH on the main steps of the arbuscular mycorrhizal fungi (AMF) life cycle resulting from lipid peroxidation; however, little is known regarding the impact of anthracene on extraradical AMF lipid metabolism. Radiolabelling experiments showed significant decreases of [1-14 C]acetate incorporation into the sterol precursors (4,4-dimethylsterols and 4␣-methylsterols) and in the total phospholipids (PL) of Rhizophagus irregularis (Blaszk., Wubet, Renker & Buscot) extraradical mycelium when grown in the presence of anthracene. These findings suggested a slowing down of the sterol and total PL biosynthesis pathways in AMF treated with anthracene. The negative impact of the organic pollutant on AMF membrane lipid biosynthesis may explain the growth inhibition of the fungus after PAH exposure. This study increases the understanding of the biochemical mechanisms involved in PAH ecotoxicity on AMF.Résumé : L'anthracène, hydrocarbure aromatique polycyclique (HAP) de faible poids moléculaire d'origine principalement anthropique, fait partie des polluants organiques persistants majeurs fréquemment rencontrés dans les sols pollués. Quelques travaux ont démontré l'effet négatif de cet HAP sur le développement du champignon mycorhizien arbusculaire (CMA), Rhizophagus irregularis (Blaszk.,Wubet, Renker & Buscot), attribué, en partie, à la peroxydation lipidique. En revanche, peu de données concernant l'impact de l'anthracène sur le métabolisme lipidique du mycélium extra-racinaire des CMA sont actuellement disponibles. Des expériences de radiomarquage ont montré des diminutions significatives de l'incorporation de l'acétate [1-14 C] dans les précurseurs stéroliques (4,4-diméthylstérols et 4␣-méthylstérols) et dans les phospholipides (PL) totaux dans le mycélium extra-racinaire de R. irregularis cultivé en présence d'anthracène. Ces résultats suggèrent un ralentissement des voies de biosynthèse des stérols et des PL chez le CMA traité avec l'anthracène. L'ensemble de nos données permettent de conclure, qu'en plus de la peroxydation lipidique, le ralentissement des voies de biosynthèse des lipides membranaires pourrait expliquer l'inhibition de croissance du CMA cultivé dans un milieu pollué par les HAP. Cette étude contribue, ainsi, à une meilleure compréhension des mécanismes biochimiques impliqués dans l'écotoxicité des HAP vis-à -vis des CMA.Mots-clés : anthracène, champignon mycorhizien arbusculaire, métabolisme lipidique, polluants, hydrocarbures aromatiques polycycliques.

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Propiconazole inhibits the sterol 14α-demethylase in Glomus irregulare like in phytopathogenic fungi

Cited by 21 publications

References 66 publications

Arbuscular mycorrhizal fungi and their response to pesticides

Arbuscular mycorrhizal fungi and their response to pesticides

Fungicides With Contrasting Mode of Action Differentially Affect Hyphal Healing Mechanism in Gigaspora sp. and Rhizophagus irregularis

Impact of anthracene on the arbuscular mycorrhizal fungus lipid metabolism

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